生物酶土壤固化剂在道路工程中的应用研究进展

生物酶固化剂作为一种环境友好的新型土壤固化材料,在路面地基材料加固方面具有重要的应用前景。本文概述了新型生物酶固化剂的性质及固化机理,并对生物酶固化剂在改良我国粉土、粉质黏土、淤泥土和膨胀土等不同土质中的具体研究和应用情况进行综述,指出了生物酶固化剂在路基应用领域存在的问题与未来发展的方向。

生物酶固化土水稳定性试验研究

生物酶固化剂作为一种绿色的土壤固化剂,由于其具有施工工艺简单、土体改良高效、经济效益好及环保等优点,而广泛应用于高速公路路基建设中。湖南省高速公路建设中,经常会遇到拟建高速公路经过高液限土区域,而高液限土体性能很难满足公路建设对路基土的要求。生物酶固化高液限土使得高液限土路基能够满足公路对路基土的强度要求,而在路基的使用过程中,环境水对路基土稳定性的影响这一问题不容忽视,路基土的水稳定性关乎高速公路的运营安全。本文针对生物酶固化路基土样的水稳定性试验,探究不同含量的生物酶的路基土饱和稳定性,为生物酶固化路基土的应用提供科学的依据。

土壤固化剂在现代路面基层和底基层中的应用

土壤固化剂是指可以对土体进行化学处理,从而改变土壤的组成和土体的工程性质,进而达到提高土质强度,改善土质压实性一种外掺剂。土壤固化剂按其性状分为固体和液体两大类,土壤固化剂是现代路面基层和底基层固化的新型化学材料,能改善和提高土壤技术性能,适用于不同类型的土质,生物酶类土壤固化剂如路易酶,它能够使土壤承载比CBR提高51%以上,并有效降低土壤渗透率,减少后期因雨水较多出现的冲蚀、车辙、隆起、洞穴等问题,大大降低工程成本,效果显著,在多个国家都有过成功的工程案例。

Investigation on Adsorption of Lithospermum erythrorhizon onto Fungal Cell Wall Polysaccharides

A culture of Lithospermum erythrorhizon adsorbed on fungal cell wall polysaccharides, a novel bio-adsorbent made from fungal cell wall, has been established in this paper. Three steps were involved in this immobilization. The first step was preparation of suspended plant cells from tightly aggregated plant cell clumps. The disassembled ratio of 0.715g·g-1 (the disassembled cells over total cells) was obtained under optimum condition for the enzymatic reaction. Then, the adsorption of plant cells onto fungal cell wall polysaccharides was conducted and the saturated capacity of 12 g cell per gram of carrier was obtained in adsorption immobilization. Finally, the culture of cells adsorbed on fungal cell wall polysaccharides was compared with that of cells entrapped in alginate or suspension cell culture. While exposed to in situ liquid paraffin extraction coupled with cell culture, the shikonin productivity of immobilized cells by adsorption was 10.67g·L-1, which was 1.8 times of that in suspension culture and 1.5 times of that entrapped in alginate.