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一株多粘类芽孢杆菌Paenibacillus polymyxa对甘薯黑斑病的生物防治效果及作用机理初探 被引量:10
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作者 王波 周涧楠 +4 位作者 黄忠勤 丁震乾 常勇 苏在兴 周兴根 《江西农业学报》 CAS 2017年第10期40-43,共4页
XZ-2是本课题组分离到的一株大豆内生多粘类芽孢杆菌。探索了该菌株对甘薯黑斑病的生物防治效果及其作用机理。研究结果表明:与清水对照相比,用XZ-2发酵原液的10~100倍稀释液处理贮藏期薯块,可以极显著地减小黑斑病病斑体积;在所有处理... XZ-2是本课题组分离到的一株大豆内生多粘类芽孢杆菌。探索了该菌株对甘薯黑斑病的生物防治效果及其作用机理。研究结果表明:与清水对照相比,用XZ-2发酵原液的10~100倍稀释液处理贮藏期薯块,可以极显著地减小黑斑病病斑体积;在所有处理中,以XZ-2发酵原液的10倍稀释液对甘薯黑斑病的防治效果最佳,达73.62%;XZ-2发酵滤液处理对甘薯黑斑病菌的菌丝形态没有明显的影响,但会导致该病菌菌丝产生厚垣孢子,且显著地抑制其分生孢子的萌发。 展开更多
关键词 多粘类芽孢杆菌 甘薯 黑斑病 生物防治效果 作用机理
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木霉菌的生防效果与菌株生物学及生化指标的相关性 被引量:2
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作者 朱虹 刘俊龙 +2 位作者 李婧梅 李增智 樊美珍 《中国森林病虫》 北大核心 2006年第2期1-3,共3页
木霉菌株的生物防治效果与菌株生物学及生化指标的相关性研究,是建立木霉菌株筛选模型和菌株改良的基础。本文以草莓灰霉病病原菌为试验对象,综合研究了木霉菌的菌落生长速度、抗生类物质产生水平、β-1,3葡聚糖苷酶浓度、几丁质酶浓度... 木霉菌株的生物防治效果与菌株生物学及生化指标的相关性研究,是建立木霉菌株筛选模型和菌株改良的基础。本文以草莓灰霉病病原菌为试验对象,综合研究了木霉菌的菌落生长速度、抗生类物质产生水平、β-1,3葡聚糖苷酶浓度、几丁质酶浓度和蛋白酶浓度5项指标与菌株所测防效的相关性。根据所测9株木霉菌对草莓灰霉病的相对防效和各菌株的生物学和生化特性,计算相关系数。结果表明,抗生类物质产生水平与菌株防治效果相关性最高,相关系数达0.97,其次为β-1,3葡聚糖苷酶0.84、生长速度0.6、几丁质酶浓度0.5,与蛋白酶浓度相关性最小。表明影响木霉菌生物防治效果的最主要的因素为菌株的抗生类物质水平和β-1,3葡聚糖苷酶浓度。 展开更多
关键词 木霉菌 生物防治效果 生物 生化指标 相关性
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苏云金芽孢杆菌NBIN863菌株对番茄根结线虫的防治效果和促生作用 被引量:11
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作者 黄大野 叶良阶 +6 位作者 刘晓艳 姚经武 曹春霞 杨妮娜 龙同 胡洪涛 杨自文 《中国蔬菜》 北大核心 2015年第10期57-60,共4页
在温室条件下,研究苏云金芽孢杆菌(Bacillus thuringiensis)NBIN863菌株对番茄根结线虫的生物防治效果,对番茄植株的促生作用及在番茄根际周围的定殖能力。结果表明:NBIN863菌株对番茄根结线虫的温室防效达到54.48%;对番茄植株具有促生... 在温室条件下,研究苏云金芽孢杆菌(Bacillus thuringiensis)NBIN863菌株对番茄根结线虫的生物防治效果,对番茄植株的促生作用及在番茄根际周围的定殖能力。结果表明:NBIN863菌株对番茄根结线虫的温室防效达到54.48%;对番茄植株具有促生作用,株高、根长和全株鲜质量分别比空白对照增加49.10%、43.69%和52.50%;该菌株能稳定在番茄根际周围定殖,接种30d后,其定殖浓度为8.37×105cfu·g-1。 展开更多
关键词 苏云金芽孢杆菌 NBIN863菌株 根结线虫 生物防治效果 促生
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酵母菌对果蔬采后病害防治的研究进展 被引量:49
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作者 张红印 蒋益虹 +2 位作者 郑晓冬 席王与芳 孙萍 《农业工程学报》 EI CAS CSCD 北大核心 2003年第4期23-27,共5页
该文对酵母菌对果蔬采后病害防治的研究现状进行了综述 ,其中包括防治效果、防治机理、防治的优势及最新研究成果。许多酵母菌对果蔬采后病害具有明显的抑制作用 ,酵母菌抑制霉菌的机理主要在于营养与空间的竞争、对病原菌的直接寄生作... 该文对酵母菌对果蔬采后病害防治的研究现状进行了综述 ,其中包括防治效果、防治机理、防治的优势及最新研究成果。许多酵母菌对果蔬采后病害具有明显的抑制作用 ,酵母菌抑制霉菌的机理主要在于营养与空间的竞争、对病原菌的直接寄生作用及诱导寄主产生抗病性。酵母菌与其它生防微生物相比 ,具有拮抗效果好、不产生毒素、可以和化学杀菌剂共同使用等优点。使用生物工程技术改造拮抗酵母菌从而提高其生防效果 ,是酵母菌对果蔬采后生物防治的最新研究领域。该文还指出了酵母菌对果蔬采后生物防治存在的问题 ,并对未来的研究提出了新的思路。 展开更多
关键词 果蔬采后病害 生物防治 酵母菌 生物防治效果与机理
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卡伍尔链霉菌TJ430的分离鉴定及抗菌活性研究
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作者 杨少彬 黄永春 +1 位作者 陈志永 梁军锋 《中国抗生素杂志》 CAS CSCD 北大核心 2015年第7期506-512,共7页
目的从土壤中分离、筛选稀有放线菌,对其产生的抗生素进行评价,以期发现新型农用抗生素。方法采用高温烘烤法对土壤中稀有放线菌进行富集,采用改良的HV培养基进行分离。对分离获得的编号为TJ430的菌株采用形态学观察法、细胞化学组分分... 目的从土壤中分离、筛选稀有放线菌,对其产生的抗生素进行评价,以期发现新型农用抗生素。方法采用高温烘烤法对土壤中稀有放线菌进行富集,采用改良的HV培养基进行分离。对分离获得的编号为TJ430的菌株采用形态学观察法、细胞化学组分分析法、生理生化及酶学特性分析、16S rDNA序列分析和DNA杂交法进行鉴定。菌株TJ430产生的抗生素水溶液在温室内进行生物防效试验。结果共计分离获得570株稀有放线菌,其中TJ430表现出优良的广谱抗真菌活性。鉴定表明TJ430是一株卡伍尔链霉菌。生物防效试验结果表明,TJ430产生的抗生素水溶液对番茄晚疫病、番茄细菌性斑点病、黄瓜菌核病及黄瓜炭疽病的防效分别为100%、79.36%,98.11%和75.79%。结论菌株TJ430产生的抗生素具有宽泛的抗菌谱和良好的抗菌活性,具备较好的开发应用前景。 展开更多
关键词 菌株鉴定 卡伍尔链霉菌 农用抗生素 生物防治效果
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Control Effect of Bio-guide Wasp Virus Missile on Ostrinia furnacalis and Its Benefits for Maize Production in Nanchong City
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作者 彭昌家 白体坤 +8 位作者 丁攀 冯礼斌 尹怀中 龙维国 杨宇衡 杨建华 何海燕 苟建华 罗怀海 《Agricultural Science & Technology》 CAS 2016年第3期662-666,共5页
In order to promote "green plant protection", reduce the administration dosage and residue of pesticides and control cost of environment pollution, and improve maize production, quality and agricultural ecological e... In order to promote "green plant protection", reduce the administration dosage and residue of pesticides and control cost of environment pollution, and improve maize production, quality and agricultural ecological environment safety. In this study, the control effect and benefit of bio-guide wasp virus missile(BGWVM) on Ostrinia furnacalis in Nanchong City was investigated by using insect light traps and combined method of field investigation and experimental demonstration. According to the results, applying 30-37 pairs of BGWVM per hectare exhibited an average control efficiency of 78.0%, and the control cost was 139.50-172.50 CNY/hm2, which was reduced by 142.50-168.00 CNY/hm^2 compared with conventional chemical control. Average maize yield in BGWVM demonstration plot was 817.8 kg/hm2 higher and improved by 11.0% compared with blank control plot. The output value was improved by 1 799.16 CNY/hm^2 and the revenue increased by 1 941.66-1 967.16CNY/hm2. During 2003-2014, the cumulative demonstration and popularization area of BGWVM technique in Nanchong City had reached 24 507.33 hm^2, and the cumulative yield increment reached 20.042 1 million kg. BGWVM application improved total output value by 44.092 6 million CNY and saved control cost by 3.492 3-4.117 2 million CNY, thus increasing the revenue by 47.584 9-48.209 8 million CNY. BGWVM application reduced 551.41 t of 5% dimehypo GG or 12.87 t of 90%monosultap WP. In addition, BGWVM technique is simple to use, labor-saving and safe for human, animals, environment and crops, with significant economic, ecological and social benefits and without any side effect, which has become an ideal substitute for controlling O. furnacalis and reducing chemical pesticides in organic agriculture industry, modern agriculture industry, production of green and pollution-free agricultural products and IBM prevention and control demonstration gardens. 展开更多
关键词 Ostrinia furnacalis Bio-guide wasp virus missile Prevention and control Effect BENEFIT Evaluation
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Application of Spodoptera litura Nucleopolyhedrovirus for Crop Pest Control
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作者 曾维爱 谭琳 +4 位作者 李小一 李宏光 谢鹏飞 黄艳宁 胡秋龙 《Agricultural Science & Technology》 CAS 2013年第9期1303-1306,共4页
The laboratory bioassay and field control efficacy of Spodoptera litura nucleopolyhedrovirus(Spli NPV) Chenzhou strain were preliminarily examined. The efficient artificial propagation method was to feed the host la... The laboratory bioassay and field control efficacy of Spodoptera litura nucleopolyhedrovirus(Spli NPV) Chenzhou strain were preliminarily examined. The efficient artificial propagation method was to feed the host larvae with virus suspension,and the average mortality of the insects was 65.0%. The death peak of the pests appeared 4-8 d after virus infection. The high temperature, high humidity and poor light could help the virus infection and propagation. Filed control efficacy of Chenzhou strain was 86.6% in laboratory, which was better than of another commercial strain. The corrected control efficacy of this strain was 88.4% the field, which was higher than that of avermectin pesticide significantly. It was detected that the occlusion body(OB) concentration of the initial virus' s stock solution was 1.03×1011OBs/ml,and it was a strong SpliNPV strain, as it showed an excellent efficacy to control the pest Spodoptera litura, and thus there will be a good prospect of application and development of this SpliNPV strain. 展开更多
关键词 Spodoptera litura nucleopolyhedrovirus Chenzhou strain BIOLOGY Control effect
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Efficacy of Several Common Green Control Measures in Rice
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作者 王万洪 周巍 +3 位作者 郑普兵 吕昂 王莹莹 代武秀 《Agricultural Science & Technology》 CAS 2017年第12期2458-2460,共3页
To promote the use of green control technology and ensure the safety of food production and agricultural products, a demonstrative test of agricultural control, physical control and biological control conducted in the... To promote the use of green control technology and ensure the safety of food production and agricultural products, a demonstrative test of agricultural control, physical control and biological control conducted in the Honghu area of Jianghan Plain during 2010-2016 with medium rice as the object. In addition, their control ef- ficacy was compared with that of conventional use of chemical agents. The results showed that the green control measures could achieve the prevention and control requirements, and the control efficacy of some of them was even better than that of conventional use of chemical agents. 展开更多
关键词 Key-words Green control Agricultural control Physical control Biological control Control efficacy
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Control Effects of 9 Fungicides on Citrus Canker 被引量:1
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作者 HUANG Ze-pei ZHOU Ya-lin +2 位作者 LONG Jian-guo MO Jun-ping SUN Wei-si 《Agricultural Science & Technology》 CAS 2021年第1期37-41,共5页
In order to screen out effective fungicides for controlling citrus canker,the control effects of 9 fungicides on Jinhong bingtang orange canker were studied.The results showed that 77%cupric calcium sulfate WP,30%copp... In order to screen out effective fungicides for controlling citrus canker,the control effects of 9 fungicides on Jinhong bingtang orange canker were studied.The results showed that 77%cupric calcium sulfate WP,30%copper oxychloride SC,46%copper hydroxide WG,30%thiodiazole-copper SC and 40%zinc thiazole SC had better comprehensive control effect on citrus canker,6%benziothiazolinone WG had an average effect,3%zhongshengmycin AS,2%kasugamycin AS and 33.5%oxine-copper SC had poor control effect.Therefore,fungicides could be used alternately or choose compounded preparation.In addition,adjuvants could be considered in use,which was an effective way to enhance efficacy,reduced dosage and delayed pesticide resistance. 展开更多
关键词 Citrus canker Copper preparation Biological agents THIAZOLES HETEROCYCLIC Control effect Efficacy test
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Experimental Model for Biological Control of Stored Grain Pests 被引量:1
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作者 Manole Traian Lupu Carmen +2 位作者 Fatu Viorel Chiriloaie Andrei Chitoran Florentin 《Journal of Agricultural Science and Technology(A)》 2015年第10期793-798,共6页
The insecticidal efficacy of the diatomaceous earth against granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae), with two essential oils for the degree of repellent activity, was evaluated in an exp... The insecticidal efficacy of the diatomaceous earth against granary weevil, Sitophilus granarius L. (Coleoptera: Curculionidae), with two essential oils for the degree of repellent activity, was evaluated in an experimental model in laboratory tests. The diatomaceous earth was obtained from three Romanian sources (Patirlagele, Urloaia and Adamclisi) and was applied at four doses of 100, 300, 500 and 900 ppm (5, 15, 25 and 45 mg, respectively) on three replicates each variant. Mortality of S. granarius adults was estimated after 7, 14 and 21 d of exposure on treated grain. The insecticidal efficacy of the examined diatomaceous earth against the granary weevil was highly influenced by exposure time, dose and essential oil type. The longer exposure times increased weevil mortality in the case of low doses (100 ppm and 300 ppm). After 60 d from the experiment initiation, the exposed grains from each variant were assessed about the progeny production. For the model proposed as bio-experiment, two essential oils in concentration of 80μL/L air and 125μL/L of Thymus vulgaris L. and Satureja hortensis L., respectively, were tested compared with untreated control in laboratory conditions. Both plant species belong to the family Lamiaceae, and the major active insecticidal compound was thymol among other providing to terpenoid class, such as eugenol and linalool. The species S. granarius chosen for experimental model was grown in the Research-Development Institute of Plant Protection (RDIPP) Bucharest bio-base. The results show that mortality induced from diatomaceous earth was at the level between 83.33% and 100% in all variants after 21 d, compared with untreated control and a standard product Silicosec. 展开更多
关键词 Diatomaceous earth integrated experimental model essential oils S. granarius.
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