引导骨再生(guided bone regeneration, GBR)是最常见的骨增量手术,而其效果受到生物屏障膜的材料特性所影响。鉴于现有的生物屏障膜无法同时满足生物相容性好、力学强度高、降解可控、生物活性佳且易操作性等临床需求,亟待研发一种新...引导骨再生(guided bone regeneration, GBR)是最常见的骨增量手术,而其效果受到生物屏障膜的材料特性所影响。鉴于现有的生物屏障膜无法同时满足生物相容性好、力学强度高、降解可控、生物活性佳且易操作性等临床需求,亟待研发一种新型生物屏障膜材料。可降解金属(biodegradable metals, BMs)是指一种预期在体内降解的金属。其中,锌基BMs因其优异的材料特性,有望成为新一代生物屏障膜材料。然而,锌基生物屏障膜仍处于基础研究阶段。在此,笔者结合团队前期的研究工作,基于今后的临床应用考虑,系统地阐析锌基生物屏障膜在生物相容性、力学性能、降解性能和生物活性四个方面的研究进展及不足,为促进其临床转化提供科学依据。展开更多
Extensive in vitro corrosion test systems have been carried out to simulate the in vivo corrosion behavior of biodegradable metallic materials. Various methods have their own unique benefits and limitations. The corro...Extensive in vitro corrosion test systems have been carried out to simulate the in vivo corrosion behavior of biodegradable metallic materials. Various methods have their own unique benefits and limitations. The corrosion mechanism of biodegradable alloys and in vitro corrosion test systems on biodegradable metallic materials are reviewed, to build a reasonable simulated in vitro test system for mimicking the in vivo animal test from the aspects of electrolyte solution selection, surface roughness influence, test methods and evaluation methodology of corrosion rate. Buffered simulated body fluid containing similar components to human blood plasma should be applied as electrolyte solution, such as simulated body fluid （SBF） and culture medium with serum. Surface roughness of samples and ratio of solution volume to sample surface area should be adopted based on the real implant situation, and the dynamic corrosion is preferred. As to the evaluation methodology of corrosion rate, different methods may complement one another.展开更多
Relapse and metastasis of tumor may occur for osteosarcoma(OS)patients after clinical resection.Conventional metallic scaffolds provide sufficient mechanical support to the defected bone but fail to eradicate recurrin...Relapse and metastasis of tumor may occur for osteosarcoma(OS)patients after clinical resection.Conventional metallic scaffolds provide sufficient mechanical support to the defected bone but fail to eradicate recurring tumors.Here we report that biodegradable magnesium(Mg)wirebased implant can inhibit OS growth.In brief,the Mg wires release Mg ions to activate the transport of zinc finger protein Snail1 from cytoplasm to cell nucleus,which induces apoptosis and inhibits proliferation of OS cells through a parallel antitumor signaling pathway of miRNA-181d-5p/TIMP3 and miRNA-181c-5p/NLK downstream.Simultaneously,the hydrogen gas evolution from Mg wires eliminates intracellular excessive reactive oxygen species,by which the growth of bone tumor cells is suppressed.The subcutaneous tumor-bearing experiment of OS cells in nude mice further confirms that Mg wires can effectively inhibit the growth of tumors and prolong the survival of tumor-bearing mice.In addition,Mg wires have no toxicity to normal cells and tissues.These results suggest that Mg implant is a potential anti-tumor scaffold for OS patients.展开更多
基金National Natural Science Foundation of China (51971014)Tianjin Municipal Natural Science Foundation (19JCQNJC02800)Beijing Municipal Natural Science Foundation (2182021)。
基金Project(2012CB619102) supported by National Basic Research Program of ChinaProject(31070847) supported by National Natural Science Foundation of China
文摘Extensive in vitro corrosion test systems have been carried out to simulate the in vivo corrosion behavior of biodegradable metallic materials. Various methods have their own unique benefits and limitations. The corrosion mechanism of biodegradable alloys and in vitro corrosion test systems on biodegradable metallic materials are reviewed, to build a reasonable simulated in vitro test system for mimicking the in vivo animal test from the aspects of electrolyte solution selection, surface roughness influence, test methods and evaluation methodology of corrosion rate. Buffered simulated body fluid containing similar components to human blood plasma should be applied as electrolyte solution, such as simulated body fluid （SBF） and culture medium with serum. Surface roughness of samples and ratio of solution volume to sample surface area should be adopted based on the real implant situation, and the dynamic corrosion is preferred. As to the evaluation methodology of corrosion rate, different methods may complement one another.
基金the National Key Research and Development Program of China(2018YFC1106600)the Interdisciplinary Program of Shanghai Jiao Tong University(ZH2018QNB07)。
文摘Relapse and metastasis of tumor may occur for osteosarcoma(OS)patients after clinical resection.Conventional metallic scaffolds provide sufficient mechanical support to the defected bone but fail to eradicate recurring tumors.Here we report that biodegradable magnesium(Mg)wirebased implant can inhibit OS growth.In brief,the Mg wires release Mg ions to activate the transport of zinc finger protein Snail1 from cytoplasm to cell nucleus,which induces apoptosis and inhibits proliferation of OS cells through a parallel antitumor signaling pathway of miRNA-181d-5p/TIMP3 and miRNA-181c-5p/NLK downstream.Simultaneously,the hydrogen gas evolution from Mg wires eliminates intracellular excessive reactive oxygen species,by which the growth of bone tumor cells is suppressed.The subcutaneous tumor-bearing experiment of OS cells in nude mice further confirms that Mg wires can effectively inhibit the growth of tumors and prolong the survival of tumor-bearing mice.In addition,Mg wires have no toxicity to normal cells and tissues.These results suggest that Mg implant is a potential anti-tumor scaffold for OS patients.
