柱状田头菇(Agrocybe cylindracea)营养菌丝的生物学特性

对一种新兴食用菌——柱状田头菇(Agrocybe cylindracea)营养菌丝的生物学特性作了较为深入的研究,结果表明:菌丝最适生长温度为25～30℃,最适 pH 为5.0左右。在供试的11种碳源中,以甘露醇为最适碳源,其次是半乳糖、葡萄糖,而对纤维二糖、羧甲基纤维素钠、甘油的利用较差。对7种氮源的利用,以蛋白胨、酵母膏明显优于其它小分子含氮物质;在无氮培养基上不能生长。硫胺素(200u/L)、肌醇(10mg/L)对菌丝生长有明显促进作用。Fe^(2+)、Cu^(2+)、Zn^(2+)对菌丝生长有一定的促进作用,Mn^(2+)、Co^(2+)、Mo^(6+)无明显效果。红霉素(1 000 u/L)、氯霉素(1000u/L)、放线菌素 D(300μg/L)对柱状田头菇菌丝生长有极显著的抑制作用,培养基中加入500μg/L制霉菌素可完全抑制其生长。

湘北黄瓜品种比较试验

为筛选出适合在湘北地区栽种的露地黄瓜品种,以引进的中研21、津优21号、天佳9号、津耘401等4个品种为试验材料进行品种比较试验。试验结果表明,天佳9号黄瓜商品性好、抗病性强、产量高、生长势强、耐高温,平均单果重为303.30 g,亩产量为7045.52 kg,比对照亩产量增加1314.66 kg,差异达到显著水平,有很好的推广应用前景,适宜在湘北地区大力推广。

Cymolomia hartigiana (Saxesen,1840)(Lepidoptera: Tortricidae) at-tacking to Abies nephrolepis Max. New to Daxing'anling,Heilongji-ang,China

An Olethreutine speciesCymolomia hartigiana (Saxesen, 1840), attacking toAbies nephrolepis Max., is reported for the first time from Daxing'anling, Heilongjiang, China. The morphological characteristics are briefly re-described with illustrations. Also its damage and biology are noted. Key words Systematics - Lepidoptera - Tortricidae - Cymolomia - Daxing'anling - China CLC number Q969.429.2 - S763.3 Document code A Foundation item: This study was carried out under the financial support by KOSEF (Korea Science & Engineering Foundation) with the program of “Korea and China Young Scientist Exchange Program” (2002–2003).Biography: *BYUN Bong-Kya (1963-), male, Ph.D., Researcher in Korea National Arboretum, Korea.Responsible editor: Chai Ruihai

Establishment of a transplantation tumor model of human osteosarcoma in chick embryo

Objective: We established a transplantation tumor model of human osteosarcoma in chick embryo, studied its morphological and biological characteristics, and observed its dynamic process of angiogenesis induction so that a simple and practical model can be provided for studying osteosarcoma. Methods: Human osteosarcoma cells at different concentrations were inoculated in chorioallantoic membrane (CAM) of chick embryos at different embryonic ages to observe the factors affecting the survival of the transplanted osteosarcoma in chick embryo, growth characteristics of the transplantation tumor, and the morphological characteristics and biological characteristics of the transplantation tumor. Results: The transplantation tumor model of human osteosarcoma in chick embryo was successfully established. It was found that the transplantation tumor was easy to grow and it showed strong angiogenesis-inducing effects. Under the light microscope, the transplantation tumor showed a similar tissue structure to human osteosarcoma. Conclusion: It is feasible to establish a transplantation tumor model of human osteosarcoma in chick embryo. The model can be easily duplicated with a simple operation, which provides a useful animal model for studying osteosarcoma.