天人合一 华夏正道

当今提倡的生理养生、心理养生、哲理养生,其实就是我国传统文化中的养生理念——下士养身,中士养气,上士养心。

Breeding of Mycoplasmal pneumonia-negative Swine Population Using Combination Therapy,Segregated Early Weaning(SEW) and Three-point Production System

In order to explore a breeding method for mycoplasmal pneumonia-negative swine population, the purifying effects of combination therapy, SEW and three-point breeding and management system on Mycoplasma hyopneumoniae were investigated in this study. After the processes of screening pregnant sows, programmed therapy for sows, SEW, three-point breeding and management in the barrier isolation system and programmed therapy for piglets, the newly-bred piglets were monitored as long as four months by serum antibody detection of Mycoplasma hyopneumoniae and fluorescent quantitative PCR of nose swabs. The results showed that when the newlybred five batches of piglets were older than 35 d, they were all negative by serum antibody detection and nose swab antigen detection. Therefore, the combination therapy, SEW and three-point production and management system can effectively purify Mycoplasma hyopneumoniae, providing theoretical basis and clinical experience for the control and purification of domestic mycoplasmal pneumonia.

Research Progress on the Biological Basis of Cereal Pollen Culture

This paper summarizes the physiological and metabolic mechanism of a series of processes in the cereal microspore culture from the angle of plant physiological metabolism, explores the physiological or cytology mechanism of several key processes, including microspore dedifferentiation and callus formation, differentiation and transformation, and sums up the current problems in this field and forecasts the direction of future development.

Ecological functions of ciliated protozoa in marine ecosystem： effects of ammonium on the population growth of Euplotes vannus

The effects of ammonium on the population growth of the marine ciliate, Euplotes vannus, were examined using ecotoxicological method, h is showed that ammonium exerts inhibitory effects on the growth of the ciliate populations in a concentration-dependent way. Statistical analvsis reveals that the population growth dynamics exposed to ammoniunt-N concentration over 100mg/L are significantly different from that in the control at P 〈 0.05 level. Linear regression determined that the 24h, 36h, 48h, 60h, 72h and 84h IC50 values of ammonium-N are 19.68, 201.51, 167.49, 47.86, 50.43 and 43.11 concentration over 100mg/L, respectively （ P 〈 0.05; pH 8.2; salinity 28 ppt; temperature, 25℃ ）. The results indicate that the tolerance to ammonium in E. vannus is considerably higher than that of the larvae or juveniles of some metozoa, such as cuhured prawns and oysters. Therefore, it is believed that the high tolerance to ammonium is necessary for eiliated protozoa to play positive roles in maintaining and improving water quality in marine ecosystems, especially in the intensive aquacuhure waters with high-level ammonium. In addition, the correlation between /C50 values and exposure time was discussed.

Science Letters:Nutritional composition of Pakistani wheat varieties

Pakistani wheat varieties are grown over a wide agro-climatic range and as such are anticipated to exhibit yield and quality differences. It is therefore necessary to investigate the nutritional status of wheat varieties in terms of biochemical and physiochemical characteristics available for food and nutritional purposes in Pakistan. The result shows that wheat grains of different varieties contain a net protein level of 9.15%～10.27%, 2.15%～2.55% total fats, 1.72%～1.85% dietary fibers, 77.65×10-6～84.25×10-6 of potassium and 7.70×10-6～35.90×10-6 of sodium ions concentration, 0.24×10-6～0.84×10-6 of phosphorus, 1.44%～2.10% ash, 31.108～43.602 g of thousand grain mass (TGM) and 8.38%～9.67% moisture contents. This study is significant in providing an opportunity to explore the available wheat varieties and to further improve their nutritional excellence and also essential for setting nutritional regulations for domestic and export purposes.

Effect of Integrated Nutrient Management on Growth and Productivity of Hybrid Rice

Sustaining rice （Oryza sativa L.） productivity at high level is a great challenge, particularly in areas where rice productivity declines in spite of following recommended nutrient management practices. Nutrient management by integrating organic manures, inorganic fertilizers and biofertilizers may play an important role in improving and sustaining rice productivity. In this study, the authors tried to evaluate the suitable proportion of organic manures and inorganic fertilizers along with biofertilizer to maximize growth and productivity of hybrid rice on sandy-loam lateritic soils of West Bengal, India. The crop having 50% recommended dose of fertilizer （RDF） ＋ 50% recommended dose of nitrogen （RDN） through mustard oil cake （MOC） and 75% RDF ＋ 25% RDN through MOC ＋ biofertilizer significantly increased plant height, number of tillers/m2, leaf area index （LAI）, dry matter accumulation （DMA） and crop growth rate （CGR） at initial and vital period of grain growth over those of 25% RDF ＋ 75% RDN through MOC and 100% RDN through MOC. The former two treatments also increased number of panicles/m2 and number of grains/panicle over those of only organic manuring （100% RDN） or only chemical fertilization （100% RDF） or 25% RDF ＋ 75% RDN through MOC. Crop with 75% RDF ＋ 25% RDN through MOC ＋ biofertilizer or 50% RDF ＋ 50% RDN through MOC produced 20.2%-33.8% higher grain yield and 11.0%-33.3% greater straw yield, and paid higher gross and net returns over other treatments. This study suggests growing hybrid rice with 75% RDF ＋ 25% RDN through MOC ＋ biofertilizer or 50% RDF ＋ 50% RDN through MOC for better growth, higher productivity and greater profit.

The metabolite profiling of coastal coccolithophorid species Pleurochrysis carterae(Haptophyta)

Pleurochrysis carterae is a calcifi ed coccolithophorid species that usually blooms in the coastal area and causes aquaculture losses. The cellular calcifi cation, blooming and many other critical species specifi c eco-physiological processes are closely related to various metabolic pathways. The purpose of this study is to apply the unbiased and non-destructive method of nuclear magnetic resonance(NMR) to detect the unknown holistic metabolite of P. carterae. The results show that NMR spectroscopic method is practical in the analysis of metabolites of phytoplankton. The metabolome of P. carterae was dominated by 26 metabolites involved in a number of dif ferent primary and secondary metabolic pathways. Organic acids and their derivatives, amino acids, sugars, nucleic aides were mainly detected. The abundant metabolites are that closely related to the process of cellular osmotic adjustment, which possibly refl ect the active ability of P. carterae to adapt to the versatile coastal niche. DMSP(dimethylsulphoniopropionate) was the most dominant metabolite in P. carterae, up to 2.065±0.278 mg/g lyophilized cells, followed by glutamate and lactose, the contents were 0.349±0.035 and 0.301±0.073 mg/g lyophilized cells respectively. Other metabolites that had the content ranged between 0.1–0.2 mg/g lyophilized cells were alanine, isethionate and arabinose. Amino acid(valine, phenylalanine, isoleucine, tyrosine), organic acid salts(lactate, succinate), scyllitol and uracil had content ranged from 0.01 to below 0.1 mg/g lyophilized cells. Trigonelline, fumarate and formate were detected in very low content(only thousandths of 1 mg per gram of lyophilized cells or below). Our results of the holistic metabolites of P. carterae are the basic references for the further studies when multiple problems will be addressed to this notorious blooming calcifying species.

Growth and Physiological Features of Cyanobacterium Anabaena sp. Strain PCC 7120 in a Glucose-Mixotrophic Culture

Mixotrophic growth is one potential mode for mass culture of microalgae and cyanobacteria particularly suitable for the production of high value bioactive compounds and fine chemicals.The typical heterocystous cyanobacterium Anabaena sp.PCC 7120 was grown in the presence of exogenous glucose in light.Glucose improved the cell growth evidently,the maximal specific growth rate under mixotrophic condition（0.38 d 1）being 1.6-fold of that of photoautotrophic growth.Mixotrophy caused a variation in cellular pigment composition,increasing the content of chlorophyll a and decreasing the contents of carotenoid and phycobiliprotein relative to chlorophyll a.Fluorescence emission from photosystem II（PSII）relative to photosystem I was enhanced in mixotrophic cells,implying an increased energy distribution in PSII.Glucokinase（EC 2.7.1.2）activity was further induced in the presence of glucose.The mixotrophic culture was scaled up in a 15 L airlift photobioreactor equipped with an inner and an outer light source.A modified Monod model incorporating the specific growth rate and the average light intensity in the reactor was developed to describe cell growth appropriately.The understanding of mixotrophic growth and relevant physiological features of Anabaena sp.PCC 7120 would be meaningful for cultivation and exploitation of this important cyanobacterial strain.

Seasonal Variations in Serological Profiles and Growth Status of Farmed and Wild Clarias gariepinus （Burchell, 1822） Obtained from Asaba, Nigeria

Seasonal variations in serological profiles and growth status of farmed and wild Clarias gariepinus were investigated. Serological profiles of Clarias gariepinus can be an effective tool for proper monitoring of stress induced by management practices in intensive fish culture, which may elicit devastating effect on fish. Spectrophotometry procedures were used to analyze serum parameters of Clarias gariepinus. No different seasonal patterns were observed for mean values of albumin, total protein, urea, cholesterol, glucose and alanine amino-transferase. However, aspartate amino-transferase showed different seasonal pattern. Levels of cholesterol and alanine amino-transferase were highly significant in farmed and wild fish, separately. Juvenile fish had high level of urea irrespective of season. Seasonal variations in water quality parameters were observed except for pH which had no seasonal pattern. The growth exponential shows b-values between -0.048 and 7.434 for Clarias gariepinus. Adult female and juvenile fish from the wild had the highest b-value and the least b-value, separately. In this study, b-values were higher in the wild fish than the farmed fish. The condition factor for Clarias gariepinus ranged from 0.422 to 0.698, and was observed to be high in juvenile fish. With a condition factor less than 1, fish may not be doing well, probably due to environmental stress. Some serological parameters varied according to season and environment of fish. Thus, serological profile of fish is an effective and sensitive tool to monitor fish response to stress factors in the environment.

中医的“三理”养生说

我国古代中医保健养生侧重生理养生，近代主要讲生理和心理养生，当代则特别强调生理、心理和哲理养生。这“三理”养生，反映了中医养生的三种不同层次。