类胰岛素生长因子-Ⅰ和胰岛素表现其功能的结构和分子基础

综述了近年来关于ＩＧＦⅠ和胰岛素表现生理功能的结构基础和分子基础研究进展．ＩＧＦⅠ和胰岛素是胰岛素家族中的２ 个重要成员， 两者的分子结构高度同源， 两者的受体相似且属同一家族， 两者的生理功能可彼此交叉， 但各自具有主要的生理功能．ＩＧＦⅠ的主要生理功能是促进细胞生长， 胰岛素的主要生理功能是促进葡萄糖的摄取和代谢． 生物大分子的功能及其表现的基础是分子结构及参与功能表现的诸多分子， 如受体、信号分子等等．

呼吸功的监测及临床意义

呼吸功是肺与胸廓扩张时所需做的功,公式为:呼吸功=压力×容积,单位为J/L或J/min,目前常用CompbellDiagram法和压力容量环面积来计算。监测时,压力探头所放位置及病人通气方式的变化表现出不同的压力容量环,所得呼吸功相应不同。辅以呼吸机的自主呼吸病人,呼吸总功WOBtot=生理呼吸功WOBphy+附加呼吸功WOBimp。WOBphy可作为拔管指标,WOBimp可指导PSV、CPAP、BiPAP等压力水平的设置,呼吸功现已成为疗效较好的通气模式的客观衡量尺度。

Study on the Soil Moisture Stress Level in Regulated Deficit Irrigation Experiment

On the basis of discussing the influencing mode of plant moisture stress on plant physiological process and the division of soil moisture availability range, the water suction values partitioning soil moisture were put forward, and then the corresponding water moistures under water stress were obtained by conversing together with characteristic curve of water moisture.

Development and Ultrastructure of the Phloem Ganglion in Bamboo Node

The development, cytological characters and ultrastructure of phloem ganglion in the nodal region of Phyllostachys edulis (Carr.) H. de Lehaie, a most economically important bamboo, were investigated and the possible physiological function of this special structure was proposed. The phloem ganglion derived directly from procambium is situated at the sites where the vascular bundle forks and is present in pairs. The phloem ganglion is spindle_like in appearance and usually consists of 4 to 6 layers. Two kinds of cells in the ganglion could be distinguished. In the middle, there are two layers of filiform cells with pointed ends so that there are no normal sieve plates. Nevertheless, there are many pits on the lateral wall of the filiform cells. The other type of cells located at both ends of the spindle which possess an intermediate form between the filiform cell and the normal sieve tube. The walls of these cells towards the filiform cells are strongly convex forming a special sieve plate. Ultrastructure study showed that cells in the ganglion are connected by enriched plasmodesma. During early differentiation, the paramural body and the ingrowth of cell wall could be observed. It indicates that the cells of phloem ganglion have the character of transfer cells. The organelles in the mature cells are mainly plastids with abundant accumulation of proteins of crystalline structure. The above_mentioned results suggest that the physiological function of the phloem ganglion is closely related with substance transport.

生理呼吸功作为呼吸机撤离指标的临床研究

目的评价生理呼吸功（ＷＯＢｐｈｙ）对机械通气患者撤机的指导意义。方法监测患者呼吸功（ＷＯＢｔ）和器械导致的附加功（ＷＯＢｉｍｐ）推算ＷＯＢｐｈｙ。不符合常规撤机标准者，以ＷＯＢｐｈｙ＜０．７０Ｊ／Ｌ为撤机标准，观察是否撤机成功。结果４１例患者中２８例符合常规撤机标准（常规组），拔管后１例再插管。１３例患者不符合常规撤机标准（非常规组），但ＷＯＢｐｈｙ均低于０．７０Ｊ／Ｌ全部拔管，其中１例再插管。非常规组ＷＯＢｔ（１．３７±０．５０Ｊ／Ｌ）和ＷＯＢｉｍｐ（０．８２±０．３７Ｊ／Ｌ）与常规组（０．９６±０．３８和０．５５±０．２７Ｊ／Ｌ）比较，差异均有显著性。但两组比较ＷＯＢｐｈｙ差异无显著性。结论以ＷＯＢｐｈｙ＜０．７０Ｊ／Ｌ作为撤机标准是安全的，有利于及时撤机拔管。

Effects of Adaptation to Elevated Salinity on Some Enzymes' Salt_tolerance in Vitro and Physiological Changes of Eelgrass

The eelgrass ( Zostera marina L.) was treated with artificial seawater (ASW) of different salinities ( 100%, 150% and 200% seawater) for 5 d. The activities of two enzymes extracted from the plant leaves were determined under a salinity grade in vitro So were the photosynthesis rates of the plants from the three treatments in the media with different salinities 100%, 150%, 200%, 300% ASW) and Some physiological data. The data showed that under increased salinities (concentrated seawater), Na+, Cl-, MDA (malon dialdehyde) and glucose contents and the osmotic potentials ( absolute value) in the leaves increased with the salinity elevation in the medium (ASW), but both K+ and free amino acid (mainly proline) contents decreased. Malate dehydrogenase (MDH) from the plant leaves under a salinity grade showed its activities (A) as follows: A(100%) (ASW) > A(150%) (ASW) > A(200%) (ASW). Phosphoenolpyruvate carboxylase (PEPC) extracted from the 100% ASW- and 200% ASW-treated plants showed similar activities (both insensitive to salinities) under the salinity grade in vitro, but the activities of PEPC from plants treated with 150% ASW were dependent oil salinity. Whether the plant is stressed at 150% ASW and can stand higher salinity than seawater needs to be studied further. Meantime, die data do not agree with the opinion that the adaptation of the eelgrass to seawater salinity is partly fulfilled by its insensitiveness to salinities in Some metabolic enzymes. It can be inferred that the lack of transpiration may be an important aspect of tire plant's tolerance to seawater salinity.

Effect of Purple Yam(Dioscorea alata L.) on Nutritional and Physiological Functions of Growing Rats

[Objective] This study was to explore the effect of purple yam on the nutritional and physiological functions of rats.[Method] Forty SD rats were randomly divided into four groups with ten rats in each group which were reared with artificial semisynthetic feed added with 10% steamed yam powder.The four groups,the casein（CK） group which was used as the control,the purple yam（PY） group,the Tiegun yam（TY） group and common Huai yam（HY） group,were supplied with feed of equal content of energy,protein and fat.The rats were pair-fed for 56 days to observe changes of relevant nutritional and physiological indices.[Result] There were no significant differences of body weight,food conversion rate and organ indices between rats in PY group and CK group.Mean corpuscular hemoglobin in PY group was significantly lower than that in CK group;hemoglobin showed no significant difference with that in CK group;eosnophils was lower than that in CK group.Low density lipoprotein-cholesterol,total cholesterol and atherogenic index in PY group were lower than that in the other three groups;anti-atherogenic index was the highest among the four groups,while showing no significant difference;triglyceride content was lower than that in CK group.Among the antioxidant indices,the activity of catalase and superoxide dismutase were higher than that in CK group,while malondialdehyde content was lower than that in CK group.[Conclusion] Purple yam showed the trend of reducing the content of blood glucose,total cholesterol,low density lipoprotein-cholesterol,triglyceride,malondialdehyde,and raising the activity of glutathione peroxidase,superoxide dismutase and catalase,thus having certain antioxidative function.

Screening of Preprocessing Method of Biolog for Soil Microbial Community Functional Diversity

As one of the main methods of microbial community functional diversity measurement, biolog method was favored by many researchers for its simple oper- ation, high sensitivity, strong resolution and rich data. But the preprocessing meth- ods reported in the literatures were not the same. In order to screen the best pre- processing method, this paper took three typical treatments to explore the effect of different preprocessing methods on soil microbial community functional diversity. The results showed that, method B＇s overall trend of AWCD values was better than A and C＇s. Method B＇s microbial utilization of six carbon sources was higher, and the result was relatively stable. The Simpson index, Shannon richness index and Car- bon source utilization richness index of the two treatments were B〉C〉A, while the Mclntosh index and Shannon evenness were not very stable, but the difference of variance analysis was not significant, and the method B was always with a smallest variance. Method B＇s principal component analysis was better than A and C＇s. In a word, the method using 250 r/min shaking for 30 minutes and cultivating at 28 ℃ was the best one, because it was simple, convenient, and with good repeatability.

别盲目追求老来瘦

问：我今年60岁，体重130斤，经常听人们说：“千金难买老来瘦”，我想减肥，请问是不是越瘦越好呢？

Systematic review of health-related quality of life after esophagectomy for esophageal cancer

This study is aimed to assess the long-term healthrelated quality of life （HRQL） of patients after esophagectomy for esophageal cancer in comparison with established norms, and to evaluate changes in HRQL during the different stages of follow-up after esophageal resection. A systematic review was performed by searching medical databases （Medline, Embase and the Cochrane Library） for potentially relevant studies that appeared between January 1975 and March 2011. Studies were included if they addressed the question of HRQL after esophageal resection for esophageal cancer. Two researchers independently performed the study selection, data extraction and analysis processes. Twenty-one observational studies were included with a total of 1282 （12-355） patients. Five studies were performed with short form-36 （SF-36） and 16 with European Organization for Research and Treatment of Cancer （EORTC） QLQ c30 （14 of them also utilized the disease-specific OESI8 or its previous version OES24）.The analysis of long-term generic HRQL with SF-36 showed pooled scores for physical, role and social function after esophagectomy similar to United States norms, but lower pooled scores for physical function, vitality and general health perception. The analysis of HRQL conducted using the Global EORTC C30 global scale during a 6-mo follow-up showed that global scale and physical function were better at the baseline. The symptom scales indicated worsened fatigue, dyspnea and diarrhea 6 mo after esophagectomy. In contrast, however, emotional function had significantly improved after 6 mo. In conclusion, short- and long-term HRQL is deeply affected after esophagectomy for cancer. The impairment of physical function may be a long-term consequence of esophagectomy involving either the respiratory system or the alimentary tract. The short- and long-term improvement in the emotional function of patients who have undergone successful operations may be attributed to the impression that they have survived a near-death experience.

Differing coping mechanisms, stress level and anorectal physiology in patients with functional constipation

AIM: To investigate coping mechanisms, constipation symptoms and anorectal physiology in 80 constipated subjects and 18 controls.METHODS: Constipation was diagnosed by Rome Ⅱ criteria.Coping ability and anxiety/depression were assessed by validated questionnaires. Transit time and balloon distension test were performed.RESULTS: 34.5% patients were classified as slow transit type of constipation. The total colonic transit time (56 h vs 10 h, P＜0.0001) and rectal sensation including urge sensation (79 mL vs 63 mL, P = 0.019) and maximum tolerable volume (110 mL vs95 mL, P = 0.03) differed in patients and controls. Constipated subjects had significantly higher anxiety and depression scores and lower SF-36 scores in all categories. They also demonstrated higher scores of'monitoring' coping strategy (14+6 vs9+3, P = 0.001),which correlated with the rectal distension sensation (P = 0.005), urge sensation (P=0.002), and maximum tolerable volume (P = 0.035). The less use of blunting strategy predicted slow transit constipation in both univariate (P = 0.01) and multivariate analysis (P = 0.03).CONCLUSION: Defective or ineffective use of coping strategies may be an important etiology in functional constipation and subsequently reflected in abnormal anorectal physiology.

Frailty and cardiovascular disease： potential role of gait speed in surgical risk stratification in older adults

Frailty is a state of late life decline and vulnerability, typified by physical weakness and decreased physiologic reserve. The epidemiology and pathophysiology of frailty share features with those of cardiovascular disease. Gait speed can be used as a measure of frailty and is a powerful predictor of mortality. Advancing age is a potent risk factor for cardiovascular disease and has been associated with an increased risk of adverse outcomes. Older adults comprise approximately half of cardiac surgery patients, and account for nearly 80% of the major complications and deaths following surgery. The ability of traditional risk models to predict mortality and major morbidity in older patients being considered for cardiac surgery may improve if frailty, as measured by gait speed, is included in their assessment. It is possible that in the future frailty assessment may assist in choosing among therapies （e.g., surgical vs. percutaneous aortic valve replacement for patients with aortic stenosis）.

Factor analysis identifies subgroups of constipation

AIM:To determine whether distinct symptom groupings exist in a constipated population and whether such grouping might correlate with quantifiable pathophysiological measures of colonic dysfunction.METHODS:One hundred and ninety-one patients presenting to a Gastroenterology clinic with constipation and 32 constipated patients responding to a newspaper advertisement completed a 53-item,wide-ranging selfreport questionnaire.One hundred of these patients had colonic transit measured scintigraphically.Factor analysis determined whether constipation-related symptoms grouped into distinct aspects of symptomatology.Cluster analysis was used to determine whether indi-vidual patients naturally group into distinct subtypes.RESULTS:Cluster analysis yielded a 4 cluster solution with the presence or absence of pain and laxative unresponsiveness providing the main descriptors.Amongst all clusters there was a considerable proportion of patients with demonstrable delayed colon transit,irritable bowel syndrome positive criteria and regular stool frequency.The majority of patients with these characteristics also reported regular laxative use.CONCLUSION:Factor analysis identified four constipation subgroups,based on severity and laxative unresponsiveness,in a constipated population.However,clear stratification into clinically identifiable groups remains imprecise.

Leptin: a multifunctional hormone

Leptin is the protein product encoded by the obese (ob)gene. It is a circulating hormone produced primarily by the adipose tissue. ob/ob mice with mutations of the gene encoding leptin become morbidly obese, infertile, hyperphagic, hypothermic,and diabetic. Since the cloning of leptin in 1994, our knowledge in body weight regulation and the role played by leptin has increased substantially. We now know that leptin signals through its receptor, OB-R, which is a member of the cytokine receptor superfamily. Leptin serves as an adiposity signal to inform the brain the adipose tissue mass in a negative feedback loop regulating food intake and energy expenditure. Leptin also plays important roles in angiogenesis, immune function, fertility and bone formation. Humans with mutations in the gene encoding leptin are also morbidly obese and respond to leptin treatment,demonstrating that enhancing or inhibiting leptin’s activities in vivo may have potential therapeutic benefits.

Insights on augmenter of liver regeneration cloning and function

Hepatic stimulator substance （HSS） has been referred to as a liver-specific but species non-specific growth factor. Gradient purification and sequence analysis of HSS protein indicated that it contained the augmenter of liver regeneration （ALR）, also known as hepatopoietin （HPO）. ALR, acting as a hepatotrophic growth factor, specifically stimulated proliferation of cultured hepatocytes as well as hepatoma cells in vitro, promoted liver regeneration and recovery of damaged hepatocytes and rescued acute hepatic failure in vivo. ALR belongs to the new Erv1/Alr protein family, members of which are found in lower and higher eukaryotes from yeast to man and even in some double-stranded DNA viruses. The present review article focuses on the molecular biology of ALR, examining the ALR gene and its expression from yeast to man and the biological function of ALR protein. ALR protein seems to be non-liver-specific as was previously believed, increasing the necessity to extend research on mammalian ALR protein in different tissues, organs and developmental stages in conditions of normal and abnormal cellular growth.

Causation mechanism of coal miners' human errors in the perspective of life events

In order to effectively decrease the safety accidents caused by coal miners’human errors,this paper probes into the causality between human errors and life events,coping,psychological stress,psychological function,physiological function based on life events’vital influence on human errors,establishing causation mechanism model of coal miners’human errors in the perspective of life events by the researching method of structural equation.The research findings show that life events have significantly positive influence on human errors,with a influential effect value of 0.7945 and a influential effect path of‘‘life events—psychological stress—psychological function—physiological function—human errors’’and‘‘life events—psychological stress—physiological function—human errors’’.

Ecological Function of Urban Green Roof and Analysis of Its Influencing Factors

Based on the analysis of the multiple ecological functions of green roofs, the main factors affecting the ecological functions of green roofs are expounded and the numerical simulation methods for quantitative calculation of ecological functions of green roofs are summarized in order to provide theoretical and method support for the construction of urban green roofs.

Importance of Monitoring of Emotional Stress and Physiological Functions of Humans during the Night Sleep

The diagnosis of disease and prescription of therapy are mainly realized through the control of physiological functions in humans during wakefulness. However, little attention is paid to evaluate the functional state of a patient in sleep. The efficiency of prescribed medicines is also determined in wakefulness. The effects of these drugs during sleep are usually out-of-sight of a physician. Human psychoemotional state during wakefulness is closely related to sleep. Various serious disturbances in physiological functions appear during the night sleep. They include cardiovascular disorders （hypertensive crisis, myocardial infarction, arrhythmias, stroke, etc.）, which may cause sudden death. A large body of evidence shows that stress is followed by sleep disorders. They serve as a factor, which contributes to the development of stress. It should be noted that sleep is the major anti-stress factor. The vicious circle is formed, which can be revealed by a complex control of human physiological functions during wakefulness and sleep. We developed a new methodology and constructed an information-and-equipment device for monitoring of vital physiological functions of humans during sleep in a real everyday life. This approach holds much promise for the diagnosis of disease and evaluation of therapeutic efficacy.

Novel mechanisms in functional dyspepsia

Functional dyspepsia （FD） is a highly prevalent but heterogeneous disorder in which multiple pathogenetic mechanisms are involved. Although there are many studies that have investigated various pathophysiologic mechanisms, the underlying casual pathways associated with FD remain obscure. The currently proposed pathophysiologic mechanisms associated with FD include genetic susceptibility, delayed as well as accelerated gastric emptying, visceral hypersensitivity to acid or mechanical distention, impaired gastric accommodation, abnormal fundic phasic contractions, abnormal antroduodenal motility, acute and chronic infections, and psychosocial comorbidity. A greater understanding of the abnormalities underlying FD may lead to improved management. The aim of this editorial is to provide a critical overview of current pathophysiologic concepts in functional dyspepsia.

Dental Variation Among Asian Colobines, with Specific Reference to the Macaques on the Same Continent

In order to discern dental morphometric variations among the Asian colobines, residuals of the colobines, derived from allometric baselines formed by the Asian macaques （Macaca）, were analyzed with Principal Components Analysis and Euclidean Distances. Results indicated that the widely accepted view that the colobines possess relatively smaller front teeth than the macaques is only the case for the first incisors. The colobines show relatively smaller molars than the macaques. Such profiles may be related to the differences in dietary preferences between the two major groups of the Asian Old World monkeys. The magnitude of such differences is not as great as usullay assumed for the two groups that contain both African and Asian taxa. In other words, the two Asian cercopithecoid groups may have homogenously been shaped by the tectonic modifications and climate alterations in the past five million years. There exist marked differences among the Asian colobines when each of the genera is compared with macaques; the dental profile reflects not only the variation in geographic distribution but also in phylogenetic divergence. Thus, the snub-nosed monkeys （Rhinopithecus） and the gray langurs （Semnopithecus） are characterized by relatively larger molars than the other colobines - larger even than those of the macaques. The differences among Asian colobines, depicted by Euclidean Distances, seems to reflect the relationship of the phylogeny and evolution between colobines and cercopithecines.