鹿复合麻醉剂对鹿生理指征、ANP和CGRP的影响

研究鹿复合麻醉剂对鹿生理指征、血浆心钠素(ANP)及降钙素基因相关肽(CGRP)的影响,以明确药物对机体作用,进而指导药物的临床应用。试验选取6只健康成年梅花鹿,肌注鹿复合麻醉剂0.04 mL/kg,注药前及注药后15、30、45、60、75、90及120 min进行收缩压(SBP)、舒张压(DBP)、平均动脉压(MAP)和心率(HR)的监测,并同步采集颈静脉血样,酶联免疫吸附法测定血浆中ANP和CGRP含量。结果表明,鹿复合麻醉剂引起麻醉期鹿血浆ANP和CGRP含量变化,使血管发生舒缩改变,导致SBP、DBP、MAP和HR产生相应的变化,苏醒后各指标均恢复到麻前水平。该药物可以应用于鹿的临床麻醉。

高呼气末正压机械通气对神经源性肺水肿患者凝血功能及生理指征的影响

目的分析高呼气末正压机械通气对神经源性肺水肿患者凝血功能及生理指征的影响。方法以我院2011年5月～2014年5月收治的100例神经源性肺水肿患者为研究对象,根据不同PEEP（高呼气末正压通气）将其分为两组,对照组PEEP为3～10 cm H_2O,观察组PEEP为11～30 cm H_2O,比较两组患者治疗前后生理指征（如心率、呼吸频率、血压、体温）、凝血功能、血气分析及1个月存活率等指标。结果观察组治疗后GOS评分、APACHE II评分、HR、MAP、RR、体温明显优于对照组（P〈0.05）。两组治疗后PT[（15.0±2.2）VS（20.5±2.1）]s、aPTT[（37.2±5.0）VS（56.8±13.5）]s、Fbg[（3.3±1.1）VS（3.8±1.4）]g/L比较差异有统计学意义（P〈0.05）。另外,观察组1个月时存活率76.0%明显较对照组36.0%高,差异有统计学意义（P〈0.05）。结论高呼气末正压机械通气能有效改善神经源性肺水肿患者生理指征及血凝功能,提高患者存活率,有临床推广及研究价值。

氧摄取率作为CABG病人生理性输血指征的评价

在美国,与冠状动脉旁路移植术(CABG)有关的用血几乎占所有同种输血的20％,但各医疗单位的用血量有很大差别。由于HIV、HCV及其他病毒标志物的检测存在窗口期,输血传播疾病的风险不可避免;临床研究表明,采用更加保守的输血指征(Hb≤7 g/dL而非Hb≤9 g/dL)。

Physiological and Biochemical Responses of Grape Yinhong Seedlings to Short-term Weaklight Stress

The plant growth and physiological and biochemical responses of root and leaves of grape Yinhong seedlings to the weak lights of 20 000,16 000,12 000,8 000 lx and the normal illumination of 25 000 lx（CK） respectively,were investigated.There was no significant dfference in growth indexes of root and leaves of grapevine seedlings between the light of 20 000 lx and CK,and the light of 16 000 lx and CK for 30 d.The chlorophyll contents,soluble protein contents,net photosynthetic rates,transpiration rates,stomatal conductance,water use efficiency and protective enzyme（CAT,POD,SOD） activities in the leaves under the lights of 20 000 lx and 16 000 lx for 30 d were all higher than those under the lights of 20 000 lx and 16 000 lx for 1 d.Under the light of 8 000 lx for 30 d,the growth indexes of root and leaves of grapevine seedlings were significantly lower than those of CK,and except for MDA content,most physiological and biochemical indexes of the leaves were significantly lower than those under the light of 8 000 lx for 1 d.Under12 000 lx,the values of most growth indexes in root and leaves and physiological and biochemical indexes in leaves were between the 16 000 lx and 8 000 lx.In conclusion,Yinhong could grow under the lights above 16 000 lx,and would be stunted by the weak light below 8 000 lx.

小儿急性腹泻病护理中运用分析

分析不同腹泻护理措施在小儿急性腹泻病护理中的运用效果。方法:于2018年5月~2022年3月研究期间选取我院收治的66例病儿进行腹泻护理分析,均患有小儿急性腹泻病,采用中心数字分层法将病儿分为甲组(33例,普通腹泻护理)与乙组(33例,全程腹泻护理),观察恢复时间指标、肠道菌群情况、病儿疼痛情况、家属满意情况、护患冲突情况。结果:护理后,乙组腹泻病儿恢复时间指标低于甲组腹泻病儿,P<0.05;护理后,乙组腹泻病儿肠道菌群情况高于甲组腹泻病儿,P<0.05;护理后,乙组腹泻病儿疼痛评分低于甲组腹泻病儿,P<0.05;护理后,乙组腹泻病儿家属满意度高于甲组腹泻病儿家属,P<0.05;护理后,乙组腹泻病儿家属护患冲突发生率低于甲组腹泻病儿家属,P<0.05。结论:小儿急性腹泻病容易导致患儿脱水,引发生理指征紊乱,对其实施全程腹泻护理后可以有效改善其症候状况,恢复其肠道菌群健康,并且减轻腹泻病儿患病期间的疼痛感,让家属对于各项护理工作更加满意。

Manila clam Venerupis philippinarum as a biomonitor to metal pollution

The Manila clam Venerupis philippinarum is a good biomonitor/bioindicator to marine metal pollution and is frequently used in aquatic toxicology. Two dominant pedigrees (white and zebra) of clam are distributed in the Bohai Sea; however, little attention has been paid to potential biological differences between these two pedigrees. In this study, we tested the sensitivity of both pedigrees to marine metal (cadmium and zinc) pollution biomonitoring and marine environmental toxicology. Results demonstrate significant biological differences in gills of white and zebra clams based on metabolic profiles and antioxidant enzyme activities. In addition, we found that hypotaurine, malonate and homarine were relatively high in white clam gills, while alanine, arginine, glutamate, succinate, 4-aminobutyrate, taurine and betaine were high in zebra clam gills. Zebra clam gills were also more sensitive to a mixture of Cd and Zn, as shown by antioxidant enzyme activities and metabolic profiles, but white clam gills could accumulate more Zn. Therefore, we suggest that the white pedigree can be used as a biomonitor to marine Zn pollution, whereas the zebra pedigree can be used for toxicology studies on Cd and Zn mixed pollution.

The expression of Elf-1 and Ki-67 and their correlations in non-small-cell lung cancer

Objective: Elf-1 is a member of the proto-oncogenes Ets-related transcription factor family and over-expressed in many human tumors, Ki-67 is an important nuclear antigen associated with cell proliferation. This study investigated the expression of Elf-1 and Ki-67 in non-small-cell lung cancer(NSCLC) and studied their correlation with the clinicopathological features. Methods: Tissue microarray from 64 cases lung cancer tissue and 10 cases normal lung tissue was constructed, immunohistochemical method was used to evaluate the protein expressions of Elf-1 and Ki-67, correlations of the expression of Elf-1 and Ki-67 to clinicopathological features of NSCLC were analyzed. Results: Expression of Elf-1 and Ki-67 in NSCLC tissues were significantly higher than in normal lung tissues(P < 0.05), the positive rate of Elf-1 and Ki-67 was 73.44% and 64.06% in NSCLC group, Overexpression of Elf-1 in NSCLC was significantly related to histopathological grading, different clinical staging and the intensity of ELF-1 expression was significantly higher in the group with lymph node metastasis than that without(P < 0.05). Overexpression of Ki-67 was also closely related to tumor differentiation, clinical stages and lymph node metastasis(P < 0.05). In addition positive correlation was found between the expressive intensity of Elf-1 and Ki-67(τ = 0.295, P = 0.018). Conclusion: The high expression and positive correlation of Elf-1 and Ki-67 in NSCLC suggest that they probably play a role in onset and progression of lung cancer, united detecting their expression could be used as an valuable molecular biological index for predicting the malignant behavior and early diagnosis of NSCLC.

神经刺激仪引导外周神经阻滞麻醉的临床效果研究

研究外周神经阻滞麻醉中采用神经刺激仪的效果。方法 选择我院2022年5月~2023年4月时间段收治的80例单侧下肢老年手术患者,通过抽签法将其分为参照组(40例,行蛛网膜下腔阻滞)与研究组(40例,行神经刺激仪引导外周神经阻滞),研究指标为生理指征、时间指标、并发症发生率。结果 SBP对比显示,研究组T1、T2、T3、T4、T5时间点均与参照组有显著差异,数值更高,P<0.05,T0与参照组无明显统计学差异P>0.05;DBP对比显示,研究组T1、T2、T3、T4、T5时间点均与参照组有显著差异,数值更高,P<0.05,T0与参照组无明显统计学差异P>0.05;HR对比显示,研究组T1、T2、T3、T4、T5时间点均与参照组有显著差异,数值更低,数值更高,P<0.05,T0与参照组无明显统计学差异P>0.05。时间指标对比显示,研究组感觉阻滞完善时间、感觉阻滞持续时间与参照组有明显统计学差异,数值更高,P<0.05;并发症对比显示,研究组与参照组无明显统计学差异P>0.05。结论 单侧下肢老年患者手术治疗当中采用神经刺激仪引导外周神经阻滞麻醉方法效果更好,持续时间更长,应用安全性更高,具有较高的临床麻醉应用价值。

手术室麻醉苏醒护理对减少腹部手术患者全麻苏醒期躁动的影响

观察腹部手术患者接受手术室麻醉苏醒护理后对全麻苏醒期躁动的影响。方法:选择我院2019年7月~2022年1月研究期间82例腹部手术患者进行手术室麻醉苏醒护理研究,根据其入院顺序随机分为甲组(41例腹部手术患者,普通护理)与乙组(41例腹部手术患者,手术室麻醉苏醒护理),研究指标为生理指征、躁动发生情况、心理健康状况、护患冲突发生率。结果:护理后,乙组腹部手术患者生理指征优于甲组腹部手术患者,P<0.05;护理后,乙组腹部手术患者躁动发生频率低于甲组腹部手术患者,P<0.05;护理后,乙组腹部手术患者心理健康状况好于甲组腹部手术患者,P<0.05;护理后,腹部手术患者护患冲突发生率低于甲组腹部手术患者,P<0.05。结论:腹部手术患者接受手术室麻醉苏醒护理,能够有效改善其麻醉苏醒期躁动情况,稳定其生理指征,减少腹部手术患者及家属与护理人员的冲突问题,应用价值高。