[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experiment...[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last.展开更多
Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ...Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ℃ on the growth and physiological changes were investigated using two maize （Zea rnays L.） inbred lines, HuangC （chilling-tolerant） and Mo17 （chilling-sensitive）. While seed priming with chitosan had no significant effect on germination percentage under low temperature stress, it enhanced germination index, reduced the mean germination time （MGT）, and increased shoot height, root length, and shoot and root dry weights in both maize lines. The decline of malondialdehyde （MDA） content and relative permeability of the plasma membrane and the increase of the concentrations of soluble sugars and proline, peroxidase （POD） activity, and catalase （CAT） activity were detected both in the chilling-sensitive and chilling-tolerant maize seedlings after priming with the three concentrations of chitosan. HuangC was less sensitive to responding to different concentrations of chitosan. Priming with 0.50% chitosan for about 60-64 h seemed to have the best effects. Thus, it suggests that seed priming with chitosan may improve the speed of germination of maize seed and benefit for seedling growth under low temperature stress.展开更多
基金Supported by Program for New Century Excellent Talents in University(NCET-04-0907)the Innovative Research Team in University (IRT0453)~~
文摘[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last.
基金Project supported by the Major Science and Technology Special Project (priority subject) of Zhejiang Province (No. 2008C12005-1)the Key Project of Education Department of Zhejiang Province (No. 20070147), China
文摘Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% （w/v） chitosan solutions at 15 ℃ on the growth and physiological changes were investigated using two maize （Zea rnays L.） inbred lines, HuangC （chilling-tolerant） and Mo17 （chilling-sensitive）. While seed priming with chitosan had no significant effect on germination percentage under low temperature stress, it enhanced germination index, reduced the mean germination time （MGT）, and increased shoot height, root length, and shoot and root dry weights in both maize lines. The decline of malondialdehyde （MDA） content and relative permeability of the plasma membrane and the increase of the concentrations of soluble sugars and proline, peroxidase （POD） activity, and catalase （CAT） activity were detected both in the chilling-sensitive and chilling-tolerant maize seedlings after priming with the three concentrations of chitosan. HuangC was less sensitive to responding to different concentrations of chitosan. Priming with 0.50% chitosan for about 60-64 h seemed to have the best effects. Thus, it suggests that seed priming with chitosan may improve the speed of germination of maize seed and benefit for seedling growth under low temperature stress.
