生皮胶原膨胀公式的推导及其应用

根据党南平衡理论 ,推导出生皮胶原的膨胀公式。运用膨胀公式判断不同的酸、碱、盐能否使生皮胶原膨胀 ,解释了各种情形下膨胀程度的差异和膨胀的抑制 ,并解释了生皮胶原的膨胀曲线。

基于生物酶制剂的绿色皮纤维分离松散技术

试图以α-淀粉酶分离松散牛皮胶原纤维,并与常规石灰法进行了对比。通过扫描电镜观察、组织学染色观察、铬鞣剂在革中的分布以及柔软度测定发现,试验组胶原纤维束的分离松散程度与对照组相当,尤其试验组所得皮革的柔软度相比对照组更佳。同时,试验组所得皮革也具有与对照组相近的力学性能和感官性能,粒面相对完整。此外,与常规工艺相比,采用α-淀粉酶处理得到的废水中的污染物负荷降低,五日生化需氧量/化学需氧量(BOD5/COD)>0.45,说明废水易于生物降解,有望成为替代石灰的环境友好型皮胶原纤维分离松散技术。

电-Fenton法和化学Fenton法对淀粉的氧化

采用电-Fenton法和化学Fenton法对淀粉进行氧化,结果可知:电-Fenton法氧化淀粉后,氧化淀粉羧基含量为1.8%,羰基含量为2.4%,黏度为10mPa·s,透光率为40.8%,均优于化学Fenton法氧化的淀粉,红外光谱也证明电-Fenton体系对淀粉氧化程度更深。应用试验也表明电-Fenton氧化淀粉作用生皮胶原,胶原收缩温度为89.0℃,而化学Fenton体系氧化淀粉的胶原收缩温度为83.0℃。

Epidermal collagenase activity and its induction by 20-hydroxyecdysone in the fiddler crab Uca pugilator

The epidermal collagenase activity and its induction by 20-hydroxyecdysone in Uca pugilator were investigated. Zymographic electrophoresis showed four bands of collagenase activity, 16, 19, 22 and 29 kDa in molecular weight, with the former two accounting for 60% and 36% , respectively, of the total collagenase activity. The collagenase activity varies during the molting cycle. Among the molt stages tested, Premolt Stage Do exhibited the highest epidermal collagenase activity for both the 16 and 19 kDa isoenzymes and, as the molt stage proceeded, the enzymatic activity of these two isoenzymes decreased, with the lowest activity for both found in Premolt Stage D3 -4 - Injection of 20-hydroxyecdysone significantly induced the activity of the 16 kDa eollagenase in the epidermis of Uca pugilator, suggesting that the activity of this isoenzyme is under molting hormone control. Although 20-hydroxyeedysone injection did not result in a statistically significant increase in the activity of the 19 kDa isoenzyme, a tendency of the induction was nonetheless demonstrated. This is the first report on epidermal collagenase activity and its induction by the molting hormone in a crustacean .