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2019—2020年酉阳县生肉样品中单核细胞增生李斯特菌监测结果 被引量:2
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作者 邓辉 匡珊珊 +4 位作者 何源 田维锋 冉茂学 陈大波 张明跃 《医学动物防制》 2022年第5期431-435,439,共6页
目的掌握酉阳县生肉样品单核细胞增生李斯特菌(单增李斯特菌,Listeria monocytogenes,LM)的污染情况、血清型别及菌株耐药情况,为该菌感染的科学防控提供依据。方法按照GB 4789.30—2016进行增菌、分离、生化鉴定;血清分型采用感染日本... 目的掌握酉阳县生肉样品单核细胞增生李斯特菌(单增李斯特菌,Listeria monocytogenes,LM)的污染情况、血清型别及菌株耐药情况,为该菌感染的科学防控提供依据。方法按照GB 4789.30—2016进行增菌、分离、生化鉴定;血清分型采用感染日本生研诊断血清,按照说明书进行;药敏试验采取K-B法进行,并进行结果判定。结果480份生肉样品检出单增李斯特菌20株,检出率为4.17%(20/480),其中12株为1/2a型,占比60.00%(12/20);6株为1/2b型,占比30.00%(6/20);2株为1/2c型,占比10.00%(2/20),未见其它型别。不同月份生肉样品单增李斯特菌检出率差异无统计学意义(χ^(2)=5.426,P>0.05);不同种类生肉样品单增李斯特菌检出率分别为生鸡肉6.45%(6/93)、生鸭肉7.14%(7/98)、生鹅肉6.12%(3/49)、生牛肉5.83%(6/103)、生猪肉6.31%(6/95)、生羊肉4.76%(2/42),差异无统计学意义(Fisher确切概率法,P=1.000)。检出的20株单增李斯特菌对头孢氨苄、头孢他啶100.00%耐药,对其它药物敏感性较高;耐2重以上药物菌株统计发现,耐4重以上2株,耐2重以上7株。结论酉阳县生肉样品单增李斯特菌的污染情况较为严重,且均为致病型别,出现多重耐药菌株。应加强食品安全监管,严防食物中毒事件发生。 展开更多
关键词 生肉样品 单核细胞增生李斯特菌 检测 污染情况 血清学分型 药敏试验 多重耐药
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Effect of Radiant Energy Vacuum on Physical and Microbial Properties of Beef Jerky
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作者 John Scott Church Carley Marie Maclntyre +4 位作者 Wade Robert Archambault Paul Edward Moote Jason Laco Cochran Timothy Douglas Durance Jonathan Douglas Van Hamme 《Journal of Food Science and Engineering》 2013年第1期33-39,共7页
To test the feasibility of radiant energy vacuum (REV) dehydration, a technology that couples microwave cooking with vacuum pressure, on beef jerky preparation, the physical and microbial properties of the final jer... To test the feasibility of radiant energy vacuum (REV) dehydration, a technology that couples microwave cooking with vacuum pressure, on beef jerky preparation, the physical and microbial properties of the final jerky product was compared to conventional preparation methods. Physical characteristics assessed using puncture and shear tests of samples prepared using REV dehydration compared to the traditional method were not statistically different (P 〈 0.05). Moisture content and water activity levels were also very similar between the two products, To test microbiological quality, samples were homogenized in a stomacher and a variety of 3 M Petrifilms were used to evaluate the microbial load. Raw beef harboured low numbers of microbes, but the post-marination pasteurization/smoking step used in both treatments eliminated all culturable microorganisms tested for. To further investigate the ability of REV dehydration to kill microbes, samples were spiked with Listeria innocua after the pasteurization/smoking step but prior to REV dehydration. Samples were taken at different time intervals for microbial enumeration, and a decimal reduction time of 1 min was calculated, with 99.99% of 1.98 × 10^7 CFU g^-1 Listeria being killed in five min. Improved drying times were observed for jerky samples prepared using the REV method offering potential energy savings during jerky preparation. 展开更多
关键词 MICROWAVE food safety DEHYDRATION PRESERVATION Listeria innocua beef jerky.
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