期刊文献+
共找到4篇文章
< 1 >
每页显示 20 50 100
生肌因子Myogenin和Myf5真核双表达载体的构建及效果检测 被引量:1
1
作者 杜朝 孙洪敏 +1 位作者 王英泽 孙国杰 《华北农学报》 CSCD 北大核心 2014年第2期13-17,共5页
为研究生肌因子如Myogenin和Myf5在调控肌特异基因表达时的作用机制,将这2个因子共转染非肌细胞,为确保转染效果,依次将这2个因子的编码序列克隆在双表达载体pVITRO2上。测序结果显示,克隆到载体的myogenin和Myf5编码区序列正确;初步的... 为研究生肌因子如Myogenin和Myf5在调控肌特异基因表达时的作用机制,将这2个因子共转染非肌细胞,为确保转染效果,依次将这2个因子的编码序列克隆在双表达载体pVITRO2上。测序结果显示,克隆到载体的myogenin和Myf5编码区序列正确;初步的功能检测表明,它们都能有效地激活各自的靶基因。 展开更多
关键词 生肌过程 双表达载体 因子
下载PDF
Akirin1基因在肌肉发生中的作用
2
作者 汪海鹏 原雪莹 倪华 《黑龙江动物繁殖》 2011年第4期11-14,共4页
Akirin1是2008年新发现的基因,其编码一个极为保守的核蛋白。研究表明,Akirin1在肌肉形成与重生中有重要作用,在C2C12细胞中的过表达能够促进生肌分化。Akirin1也能参与果蝇和小鼠的先天免疫反应,果蝇S2细胞缺失该基因后抵抗革兰氏阴性... Akirin1是2008年新发现的基因,其编码一个极为保守的核蛋白。研究表明,Akirin1在肌肉形成与重生中有重要作用,在C2C12细胞中的过表达能够促进生肌分化。Akirin1也能参与果蝇和小鼠的先天免疫反应,果蝇S2细胞缺失该基因后抵抗革兰氏阴性菌侵染的能力下降。尽管对Akirin1的研究日益增多,但是该基因的作用机制及相关表达调控尚未完全阐明。本文综述目前国内外对Akirin1的研究进展。 展开更多
关键词 Akirin1 MYOSTATIN 生肌过程 肉重
下载PDF
Injury and regeneration of intramuscular connective tissue subjected to various regimes of distraction
3
作者 赵黎 许崧杰 孙效棠 《Chinese Journal of Traumatology》 CAS 2008年第2期67-71,共5页
Objective: To investigate the effect on intramuscular connective tissue and passive range of joint motion by the stress produced in limb lengthening. Methods: An animal model of limb lengthening was established in... Objective: To investigate the effect on intramuscular connective tissue and passive range of joint motion by the stress produced in limb lengthening. Methods: An animal model of limb lengthening was established in the tibia of rabbits. Distraction was initiated at a rate of 1 mm/d and 2 mm/d in two steps respectively, and both proceeded until 10% and 20% of the tibia length was achieved. Muscle samples were harvested at the time when distraction ended and at the 4th week of consolidation after the distraction. Scanning electron microscope was applied to observe the morphological changes of the perimysium. The goniometer, which we made for this study, was used to measure the passive range of joint motion. Results: The collagen fibers were partitioned in bundles, crimped and interconnected closely and orderly. In the regime of 1 mm/d distraction with 10% lengthening, no apparent changes of the collagen fiber and passive range of joint motion was demonstrated. When tibia was increased to 20%, the crimped fibers showed a tendency of being straightened while the passive range of joint motion was reduced. The findings remained the same at the 4th week ofconsolidation. In the regime of 2 mm/d distraction with 10% lengthening, the crimped structure of the collagen fibers in the perimysium disappeared and the fibers were almost straightened. Additionally, the interconnection of the collagen fibers became loosened and interstice was presented among the fibers. At the 4th week of consolidation, the restoration to the original crimped structure was not completed. When the lengthening ratio was increased to 20%, the collagen fibers were straightened completely. This condition remained unchanged throughout all 4 weeks. The passive range of joint motion was reduced dramatically in the regime of 2 mm/d distraction. Conclusion: The ultrastructure of perimysium and the passive range of joint motion in the regime of lmm/d lengthening shows the condition closest to the normal ones. The regime of 2 mm/d lengthening may cause an apparent change in the ultrastructure of perimysium and passive range of joint motion. 展开更多
关键词 Stress EXTREMITIES Ilizarov technique Range of motion articular
原文传递
Morphogenesis of T-tubules in heart cells: the role of junctophilin-2 被引量:4
4
作者 HAN Jing WU HaoDi +1 位作者 WANG QiWei WANG ShiQiang 《Science China(Life Sciences)》 SCIE CAS 2013年第7期647-652,共6页
The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not ... The T-tubule (TT) system forms the structural basis for excitation-contraction coupling in heart and muscle cells. The morphogenesis of the TT system is a key step in the maturation of heart cells because it does not exist in neonatal cardiomyocytes. In the present study, we quantified the morphological changes in TTs during heart cell maturation and investigated the role of junctophilin-2 (JP2), a protein known to anchor the sarcoplasmic reticulum (SR) to TT, in changes to TT morphological parameters. Analysis of confocal images showed that the transverse elements of TTs increased, while longitudinal elements decreased during the maturation of TTs. Fourier transform analysis showed that the power of ~2 m spatial components increased with cardiomyocytes maturation. These changes were preceded by increased expression of JP2, and were reversed by JP2 knockdown. These findings indicate that JP2 is required for the morphogenesis of TTs during heart development. 展开更多
关键词 T-TUBULES junctophilin-2 CARDIOMYOCYTES MORPHOGENESIS
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部