Different studies show that the altitude regions of the State of Santa Catarina have great potential for the production of grapevine varieties. The aim of this study was to characterize the phenological development, d...Different studies show that the altitude regions of the State of Santa Catarina have great potential for the production of grapevine varieties. The aim of this study was to characterize the phenological development, determine the thermal requirements in degree-days （DD）, the index heliothermic （IH） and climatic variables during the vegetative and productive development of varieties Merlot and Cabernet Sauvignon grown in Campo Belo do Sul （27°40′04″ S, 50°44′48″ W, altitude 950 m a.s.l.） and Sao Joaquim （28°15′13″ S, 49°57′02″ W, altitude 1,400 m a.s.l.） during 2012/2013 cycle. Phenological stages evaluated were bud break, full bloom, veraison and maturity. Higher temperatures, global radiation, PAR and lower volumes of rainfall were the main climatic parameters that differentiated Campo Belo do Sul from Sao Joaquim. Due to occurrence of higher temperatures, Campo Belo do Sul presented thermal summation around 40% higher than Sao Joaquim. The low temperatures of Sao Joaquim extended the period of grape ripening and were responsible for the highest levels of titratable acidity and the higher concentrations of anthocyanins and total polyphenols in both varieties. The results show that in the altitude ranges studied （900 and 1400 m）, climatic behavior and viticultural performance of Cabernet Sauvignon and Merlot were suitable for producing quality wines.展开更多
Developmental potency of primitive and embryonic ectoderm cells from 4.50-day to 6.25-day post-coitum (p.c.) mouse embryos and primordial germ cells from 12.50-day p.c.male genital ridges of fetal mice were studied by...Developmental potency of primitive and embryonic ectoderm cells from 4.50-day to 6.25-day post-coitum (p.c.) mouse embryos and primordial germ cells from 12.50-day p.c.male genital ridges of fetal mice were studied by direct introducing them into 3.50-day p.c.blastocysts.Sixteen (61.5) overt chimaeras out of 26(50%) offsprings were obtained after transfer of 52 blastocysts injected with 4.50-day primitive ectoderm cells;four (16.0%) overt chimaeras were obtained out of 25 (51.0%) offsprings with 4.75-day primitive ectoderm cells from 49 transferred blastocysts.However,no overt chimaera was obtained with either 5.25-day or 6.25-day embryonic ectoderm cells or 12.50-day male primordial germ cells.GPI analysis of mid-gestation conceptuses developed from injected blastocysts showedthat 5.25-day embryonic ectoderm cells could only contributed to yolk sac of conceptus.Results suggested that implantation acts as a trigger for the determination of primitive ectoderm cells,and their developmental potency becomes limited within a short period of time in normal development.展开更多
文摘Different studies show that the altitude regions of the State of Santa Catarina have great potential for the production of grapevine varieties. The aim of this study was to characterize the phenological development, determine the thermal requirements in degree-days （DD）, the index heliothermic （IH） and climatic variables during the vegetative and productive development of varieties Merlot and Cabernet Sauvignon grown in Campo Belo do Sul （27°40′04″ S, 50°44′48″ W, altitude 950 m a.s.l.） and Sao Joaquim （28°15′13″ S, 49°57′02″ W, altitude 1,400 m a.s.l.） during 2012/2013 cycle. Phenological stages evaluated were bud break, full bloom, veraison and maturity. Higher temperatures, global radiation, PAR and lower volumes of rainfall were the main climatic parameters that differentiated Campo Belo do Sul from Sao Joaquim. Due to occurrence of higher temperatures, Campo Belo do Sul presented thermal summation around 40% higher than Sao Joaquim. The low temperatures of Sao Joaquim extended the period of grape ripening and were responsible for the highest levels of titratable acidity and the higher concentrations of anthocyanins and total polyphenols in both varieties. The results show that in the altitude ranges studied （900 and 1400 m）, climatic behavior and viticultural performance of Cabernet Sauvignon and Merlot were suitable for producing quality wines.
文摘Developmental potency of primitive and embryonic ectoderm cells from 4.50-day to 6.25-day post-coitum (p.c.) mouse embryos and primordial germ cells from 12.50-day p.c.male genital ridges of fetal mice were studied by direct introducing them into 3.50-day p.c.blastocysts.Sixteen (61.5) overt chimaeras out of 26(50%) offsprings were obtained after transfer of 52 blastocysts injected with 4.50-day primitive ectoderm cells;four (16.0%) overt chimaeras were obtained out of 25 (51.0%) offsprings with 4.75-day primitive ectoderm cells from 49 transferred blastocysts.However,no overt chimaera was obtained with either 5.25-day or 6.25-day embryonic ectoderm cells or 12.50-day male primordial germ cells.GPI analysis of mid-gestation conceptuses developed from injected blastocysts showedthat 5.25-day embryonic ectoderm cells could only contributed to yolk sac of conceptus.Results suggested that implantation acts as a trigger for the determination of primitive ectoderm cells,and their developmental potency becomes limited within a short period of time in normal development.
