Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied ...Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization. Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of prolifera-tive mesangium. Increased expression of α2 and β1 proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of α1, α2, β1 and γ1 increased to different degrees in glomeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:① The quantitative and qualitative alterations of laminin chains' distribution were found in the measngial proliferative glomeruli. The proliferative mesangial cells were the origins of abnormal accumulation and expression of laminins. ② These changes may be the basis of the progresses of MsPGN.展开更多
基金Supported by Grant from the Natural Science Foundation of Education Committee of Jiangsu Province(96047)
文摘Objective: To investigate the role of laminins in the pathogensis of mesangial pro-liferative glomeruonephritis (MsPGN) in children. Methods: Eighteen renal biopsy specimens of MsPGN and 6 normal kidneys were studied by means of immunohistochemistry and in situ hybridization. Results: ① Protein of α1 chain and γ1 chain of laminin increased around the segments of prolifera-tive mesangium. Increased expression of α2 and β1 proteins was found in the segments with mesangial proliferation whereas the β2 chain expression decreased in these areas. ② The mRNA expression of α1, α2, β1 and γ1 increased to different degrees in glomeruli with mesangial proliferation. But no difference was detected among Mild, Moderate, and Severe MsPGN. Conclusion:① The quantitative and qualitative alterations of laminin chains' distribution were found in the measngial proliferative glomeruli. The proliferative mesangial cells were the origins of abnormal accumulation and expression of laminins. ② These changes may be the basis of the progresses of MsPGN.
