The researches have been carried out in 2011-2012, in the experimental fields of Yuryev Plant Production Institute Kharkiv, Ukraine. The forms of ＂00＂ type （a low content of erucic acid and glucosinolates） and sel...The researches have been carried out in 2011-2012, in the experimental fields of Yuryev Plant Production Institute Kharkiv, Ukraine. The forms of ＂00＂ type （a low content of erucic acid and glucosinolates） and self-pollinated lines--110M-10, 127M-10, 132M-10 were studied; the forms of＂0＋＂ type--selfed lines 124-10, 221-10, 305-10; the forms of＂＋0＂ type--cultivars Victor, Janus, Emerald; forms of ＂＋＋＂ type--cultivars Fyedorovskiy, Marens, Uspikh, as well as, self-incompatible forms 5C, 15C-10, 32C-10 and the original sterile form of mutant origin were used. Viability in stigmas of the pistil lasted 3-7 days, in the forms of ＂＋0＂, ＂＋＋＂ type, 7-15 days in the cultivars and forms of ＂0＋＂, ＂00＂ type, 14-21 days in the self-incompatible and sterile forms. A fast loss of sensitivity to pollen by pistil＇s stigma was observed in the forms of＂＋0＂ and ＂＋＋＂ types. On the contrary, in self-incompatible and sterile forms was observed a gradual increase of sensitivity of pistil＇s stigma to pollination and then its gradual fall. A maximum of sensitivity of a pistil＇s stigma to pollen in ＂00＂ and ＂0＋＂ forms was on the 3rd-6th day after castration.展开更多
Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogeno...Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogenous H2 S generation, the promoter sequences of L-cysteine desulfhydrase(LCD), D-cysteine desulfhydrase(DCD1,DCD2), desulfhydrase(DES) and nitrogen fixation synthetase(NFS1, NFS2) were analyzed. They are all found to contain a W-box, a characteristic core binding site for the plant WRKY transcription factors, which have important roles in the plant's responses to numerous stresses by modifying the expression patterns of their target genes. An electrophoretic mobility shift assay indicated that WRKY18 and WRKY60 interacted with the W-box in the promoters of the LCD, DCD1, DCD2, DES and NFS2 genes, whereas WRKY40 bound to the W-box of the NFS1 promoter. The expression levels of the LCD, DES and DCD1 genes were up-regulated, but the DCD2 was downregulated in the plants with WRKY18, WRKY40 or WRKY60 mutations. The plants with double and triple mutations of WRKY18, WRKY40 and WRKY60 had a higher rate of H2 S production during cadmium stress and were more resistant to the cadmium stress than the wild type or single mutants. These results suggest that WRKY transcription factors regulate the H2 S signaling pathway in plants, allowing them to cope with cadmium stress.展开更多
文摘The researches have been carried out in 2011-2012, in the experimental fields of Yuryev Plant Production Institute Kharkiv, Ukraine. The forms of ＂00＂ type （a low content of erucic acid and glucosinolates） and self-pollinated lines--110M-10, 127M-10, 132M-10 were studied; the forms of＂0＋＂ type--selfed lines 124-10, 221-10, 305-10; the forms of＂＋0＂ type--cultivars Victor, Janus, Emerald; forms of ＂＋＋＂ type--cultivars Fyedorovskiy, Marens, Uspikh, as well as, self-incompatible forms 5C, 15C-10, 32C-10 and the original sterile form of mutant origin were used. Viability in stigmas of the pistil lasted 3-7 days, in the forms of ＂＋0＂, ＂＋＋＂ type, 7-15 days in the cultivars and forms of ＂0＋＂, ＂00＂ type, 14-21 days in the self-incompatible and sterile forms. A fast loss of sensitivity to pollen by pistil＇s stigma was observed in the forms of＂＋0＂ and ＂＋＋＂ types. On the contrary, in self-incompatible and sterile forms was observed a gradual increase of sensitivity of pistil＇s stigma to pollination and then its gradual fall. A maximum of sensitivity of a pistil＇s stigma to pollen in ＂00＂ and ＂0＋＂ forms was on the 3rd-6th day after castration.
基金supported by the National Natural Science Foundation of China(31300236,31372085 and31400237)Shanxi Province Science Foundation for Youths(2014021026-1)Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(2013103)
文摘Hydrogen sulfide(H2S) is a gasotransmitter playing a vital role in response to biotic and abiotic stress for plants. In order to understand the transcriptional regulation of the genes that are responsible for endogenous H2 S generation, the promoter sequences of L-cysteine desulfhydrase(LCD), D-cysteine desulfhydrase(DCD1,DCD2), desulfhydrase(DES) and nitrogen fixation synthetase(NFS1, NFS2) were analyzed. They are all found to contain a W-box, a characteristic core binding site for the plant WRKY transcription factors, which have important roles in the plant's responses to numerous stresses by modifying the expression patterns of their target genes. An electrophoretic mobility shift assay indicated that WRKY18 and WRKY60 interacted with the W-box in the promoters of the LCD, DCD1, DCD2, DES and NFS2 genes, whereas WRKY40 bound to the W-box of the NFS1 promoter. The expression levels of the LCD, DES and DCD1 genes were up-regulated, but the DCD2 was downregulated in the plants with WRKY18, WRKY40 or WRKY60 mutations. The plants with double and triple mutations of WRKY18, WRKY40 and WRKY60 had a higher rate of H2 S production during cadmium stress and were more resistant to the cadmium stress than the wild type or single mutants. These results suggest that WRKY transcription factors regulate the H2 S signaling pathway in plants, allowing them to cope with cadmium stress.
