Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the fi...Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L·1,yeast powder 25.50 g·L·1,NaH2PO4 0.4 g·L·1 and K2HPO4 0.4 g·L·1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min·1 for 168 h.The yield of IPS is 737.93 mg·L·1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.展开更多
In this paper, we adopted simulation method to discuss influences of litter layer on plants habitat in grassland. Results indicated that ground surface evaporation, soil moisture, surface temperature, soil pH, soil bu...In this paper, we adopted simulation method to discuss influences of litter layer on plants habitat in grassland. Results indicated that ground surface evaporation, soil moisture, surface temperature, soil pH, soil bulk density and soil porosity were all strongly related to the litter quantity. Potassium （K） and organic materials in the soil covered by litter layer were higher than those in the soil uncovered by litter layer. With 100 g.ln-z increase of litter, the percentage of organic materials increased by 17.9%, nitrogen （N） increased by 7.6%, phosphor （P） increased by 26.4%, and K increased by 3.8%. With the litter accumulation amounting up to 600 g-m-2, the percentage of organic materials increased by 1.8 times, N increased by 81.5%, P increased by 1.8 times and K increased by 26.4%. According to the expected coefficient method of optimization, a mathematical model was established about the optimal accumulation quantity of litter.展开更多
With the goal of model fitting species abundance distribution patterns of the tree,shrub and herb layers of the natural Toona ciliata community in Xingdoushan Nature Reserve,Enshi Autonomous Prefecture,Hubei Province,...With the goal of model fitting species abundance distribution patterns of the tree,shrub and herb layers of the natural Toona ciliata community in Xingdoushan Nature Reserve,Enshi Autonomous Prefecture,Hubei Province,we used the data collected from the field survey and employed different ecological niche models.The models tested were the broken stick model(BSM),the overlapping niche model(ONM)and the niche preemption model(NPM),as well as three statistic models,the log-series distribution model(LSD),the log-normal distribution model(LND)and the Weibull distribution model(WDM).To determine the fitted model most suitable to each layer,the fitting effects were judged by criteria of the lowest value of Akaike Information Criterion(AIC),Chi-square and the K-S values with no significant difference(P>0.05)between the theoretical predictions and observed species abundance distribution values.The result showed:(1)The fitting suitability and goodness of fit of the tree,shrub and herb layers by using the three ecological niche models were ranked as:NPM>BSM>ONM.Of the three statistical models,by accepting the fitting results of the three layers,WDM was the best fitting model,followed by LND.By rejecting the fitting tests of the herb layer,LSD had the worst fitting effect.The goodness of the statistical models was ranked as:WDM>LND>LSD.In general,the statistical models had better fitting results than the ecological models.(2)T.ciliata was the dominant species of the tree layer.The species richness and diversity of the herb layer were much higher than those of either the tree layer or the shrub layer.The species richness and diversity of the shrub layer were slightly higher than those of the tree layer.The community evenness accorded to the following order:herb>shrub>tree.Considering the fitting results of the different layers,different ecological niche models or statistical models with optimal goodness of fit and ecological significance can be given priority to in studying the species abundance distribution patterns of T.ciliata communities.展开更多
基金Supported by the National High Technology Research and Development Program of China (2007AA021506)the Natural Science Foundation of Zhejiang Province (R207609)the Research Project of Science and Technology of Zhejiang Province,China (2005C23027)
文摘Cordyceps ophioglossoides is a valuable traditional medicinal material.We have found that intracellular polysaccharide(IPS) is the major biologically active ingredient in Cordyceps ophioglossoides.This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides.The optimal medium for IPS production consists of glucose 54.50 g·L·1,yeast powder 25.50 g·L·1,NaH2PO4 0.4 g·L·1 and K2HPO4 0.4 g·L·1.The suggested culture conditions are 24 ℃,initial pH 4.5 with a rotary speed of 120 r·min·1 for 168 h.The yield of IPS is 737.93 mg·L·1,which is 50% higher than the yield under the conditions prior to optimization.The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay.The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.
基金Acknowledgements： This research work was supported by the National Natural Science Foundation of China （No. 30590382 and No. 30570273） and Science Foundation for Young Teachers of Northeast Normal University （No. 20070502）.
文摘In this paper, we adopted simulation method to discuss influences of litter layer on plants habitat in grassland. Results indicated that ground surface evaporation, soil moisture, surface temperature, soil pH, soil bulk density and soil porosity were all strongly related to the litter quantity. Potassium （K） and organic materials in the soil covered by litter layer were higher than those in the soil uncovered by litter layer. With 100 g.ln-z increase of litter, the percentage of organic materials increased by 17.9%, nitrogen （N） increased by 7.6%, phosphor （P） increased by 26.4%, and K increased by 3.8%. With the litter accumulation amounting up to 600 g-m-2, the percentage of organic materials increased by 1.8 times, N increased by 81.5%, P increased by 1.8 times and K increased by 26.4%. According to the expected coefficient method of optimization, a mathematical model was established about the optimal accumulation quantity of litter.
基金Public Welfare Research Project of Department of Science and Technology in Hubei Province(40 2012DBA40001)Scientific Research Project of Department of Education in Hubei Province(B20160555)
文摘With the goal of model fitting species abundance distribution patterns of the tree,shrub and herb layers of the natural Toona ciliata community in Xingdoushan Nature Reserve,Enshi Autonomous Prefecture,Hubei Province,we used the data collected from the field survey and employed different ecological niche models.The models tested were the broken stick model(BSM),the overlapping niche model(ONM)and the niche preemption model(NPM),as well as three statistic models,the log-series distribution model(LSD),the log-normal distribution model(LND)and the Weibull distribution model(WDM).To determine the fitted model most suitable to each layer,the fitting effects were judged by criteria of the lowest value of Akaike Information Criterion(AIC),Chi-square and the K-S values with no significant difference(P>0.05)between the theoretical predictions and observed species abundance distribution values.The result showed:(1)The fitting suitability and goodness of fit of the tree,shrub and herb layers by using the three ecological niche models were ranked as:NPM>BSM>ONM.Of the three statistical models,by accepting the fitting results of the three layers,WDM was the best fitting model,followed by LND.By rejecting the fitting tests of the herb layer,LSD had the worst fitting effect.The goodness of the statistical models was ranked as:WDM>LND>LSD.In general,the statistical models had better fitting results than the ecological models.(2)T.ciliata was the dominant species of the tree layer.The species richness and diversity of the herb layer were much higher than those of either the tree layer or the shrub layer.The species richness and diversity of the shrub layer were slightly higher than those of the tree layer.The community evenness accorded to the following order:herb>shrub>tree.Considering the fitting results of the different layers,different ecological niche models or statistical models with optimal goodness of fit and ecological significance can be given priority to in studying the species abundance distribution patterns of T.ciliata communities.
