从广西玉林土壤中分离获得一株淡色生赤壳菌YLZ42,其发酵产物对蜡样芽孢杆菌等具有良好的抑制作用。以蜡样芽孢杆菌为指示菌对该菌株发酵产物的活性特性进行了研究,结果表明该菌发酵液的抑菌活性在40~60℃温度范围内比较稳定,60℃加热1...从广西玉林土壤中分离获得一株淡色生赤壳菌YLZ42,其发酵产物对蜡样芽孢杆菌等具有良好的抑制作用。以蜡样芽孢杆菌为指示菌对该菌株发酵产物的活性特性进行了研究,结果表明该菌发酵液的抑菌活性在40~60℃温度范围内比较稳定,60℃加热1 h,活性损失小于7%;在p H 2~7范围内抑菌活性稳定,p H大于7时,活性损失较大,在p H 8条件下处理12 h活性损失已达到64%;发酵液适宜在酸性条件下保存,p H 4的发酵液放置15 d后活性损失小于5%;活性物质对紫外线不敏感,经紫外照射24 h后抑菌活性基本不变。然后采用萃取、薄层制备、HPLC制备等提取分离方法得到了抑菌活性物质的纯品,分子量为513,与相关文献中报道的活性代谢产物的分子量均不同。展开更多
The sponge-associated fungus Nectria sp.HLS206,which belongs to the order of Hypocreales,was isolated from the marine sponge Gelliodes carnosa collected from the South China Sea.The secondary metabolites of the fungus...The sponge-associated fungus Nectria sp.HLS206,which belongs to the order of Hypocreales,was isolated from the marine sponge Gelliodes carnosa collected from the South China Sea.The secondary metabolites of the fungus were isolated.From the solid cultures,five compounds were purified,which are chalmicrin（1）,hypocrealesate（2）,fucosterol（3）,stigmasterol（4） and β-sitosterol（5）.The structures of the 5 compounds were elucidated by ESI-MS,1D and 2D NMR spectra（COSY,HMQC and HMBC）.To the best of our knowledge,it is the first time compound 1 was isolated from the order of Hypocreales,and the spectral data of compound 2 are also firstly reported.展开更多
文摘从广西玉林土壤中分离获得一株淡色生赤壳菌YLZ42,其发酵产物对蜡样芽孢杆菌等具有良好的抑制作用。以蜡样芽孢杆菌为指示菌对该菌株发酵产物的活性特性进行了研究,结果表明该菌发酵液的抑菌活性在40~60℃温度范围内比较稳定,60℃加热1 h,活性损失小于7%;在p H 2~7范围内抑菌活性稳定,p H大于7时,活性损失较大,在p H 8条件下处理12 h活性损失已达到64%;发酵液适宜在酸性条件下保存,p H 4的发酵液放置15 d后活性损失小于5%;活性物质对紫外线不敏感,经紫外照射24 h后抑菌活性基本不变。然后采用萃取、薄层制备、HPLC制备等提取分离方法得到了抑菌活性物质的纯品,分子量为513,与相关文献中报道的活性代谢产物的分子量均不同。
基金National Science and Technology Project of China(Grant No.2009ZX09301-003-4-1)
文摘The sponge-associated fungus Nectria sp.HLS206,which belongs to the order of Hypocreales,was isolated from the marine sponge Gelliodes carnosa collected from the South China Sea.The secondary metabolites of the fungus were isolated.From the solid cultures,five compounds were purified,which are chalmicrin（1）,hypocrealesate（2）,fucosterol（3）,stigmasterol（4） and β-sitosterol（5）.The structures of the 5 compounds were elucidated by ESI-MS,1D and 2D NMR spectra（COSY,HMQC and HMBC）.To the best of our knowledge,it is the first time compound 1 was isolated from the order of Hypocreales,and the spectral data of compound 2 are also firstly reported.
