战场C^4ISR中的体系成熟度生长模型

通过比较体系和一般复杂巨系统概念之间的差异,提出了一个用来刻划和分析战场C4ISR领域体系的成熟度生长模型,指出一体化是体系生长过程达到的一种较高级的状态。结合美军的区域防空体系发展,讨论了C4ISR中体系认知和研制中容易犯的错误,揭示其体系成长和发展规律,指出企图通过某个独立系统的构建来操控一个体系,不符合体系发展规律,类似的努力也难以成功。

以“润志教育”赋能学校高质量发展

新时代,要加快推进教育高质量发展,加快建设教育强国,办好人民满意的教育。江苏省徐州市华润小学秉持“润志教育”理念,深刻理解和践行为党育人、为国育才的历史使命,通过多种创新路径促进学校的高质量发展。具体举措有:回溯红色基因,构建高位引领的“润志教育”理念体系;坚定立德树人,构建融会贯通的“润志生长”课程体系;赋能共进,构建协同发展的“润志人文”治理体系。

次生诱导拟南芥的茎、下胚轴解剖结构变化的初步研究

近年利用草本模式植物拟南芥开展木材发育的基因功能研究逐渐开展起来。草本植物与木本植物的发育与结构存在一定差别,但利用次生诱导促进拟南芥的次生结构发育,可模拟"木材"的形成,以便开展木材发育的基因功能研究。本研究利用次生诱导体系培育拟南芥,并与未次生诱导的拟南芥进行解剖结构比较,分析了拟南芥次生诱导后产生的组织结构变化。结果表明:次生诱导后拟南芥下胚轴部位产生大量次生维管组织,可在一定程度上模拟木材发育,可作为木材发育研究的基础研究体系。

“紧凑城市”理念下的建筑高度控制探索——以西安曲江新区高度控制研究为例

面对日益紧张的人地关系,建筑高度的适度提升不仅有利于土地和资源的集约利用,而且为人类生活活动空地的预留提供了更大的可能性,具有保护环境和生态、促进社会公平、体现城市环境友好的可持续发展意义。西安曲江新区尝试通过建筑高度的"生长力"评价对不同地段建筑高度的发展潜力进行判断,在城市立体空间生长综合效益的量化平衡基础上,以节约土地、提高城市空间利用效益为目标,构建城市建筑高度"生长力"评价指标体系,探讨"紧凑城市"理念下的建筑高度控制方法。

概念教学,守朴日新——《线段、射线、直线(1)》教学片段与思考

数学概念教学应注重其本质,即概念的生长过程,这一内容是初中数学教学研究的重要课题。文章结合一课例具体阐述数学概念的三种类型分别是如何自然质朴地生成的,论述说明其巧妙创新地被学生发现、发明和发展这一过程,以及这三种类型的概念在同一节课例的设计中相互融合、相互生长最终构成新的数学结构、数学系统。

The promoting molecular mechanism of alphafetoprotein on the growth of human hepatoma Bel7402 cell line

AIM: The goal of this study was to characterize the AFP receptor, its possible signal transduction pathway and its proliferative functions in human hepatoma cell line Bel 7402. METHODS: Cell proliferation enhanced by AFP was detected by MTT assay, 3H-thymidine incorporation and S-stage percentage of cell cycle analysis. With radioactive labeled 125I-AFP for receptor binding assay; cAMP accumulation, protein kinase A activity were detected by radioactive immunosorbent assay and the change of intracellular free calcium (Ca2+i) was monitored by scanning fluorescence intensity under TCS-NT confocal microscope. The expression of oncogenes N- ras, p 53, and p21( ras ) in the cultured cells in vitro were detected by Northern blotting and Western blotting respectively. RESULTS: It was demonstrated that AFP enhanced the proliferation of human hepatoma Bel 7402 cell in a dose dependent fashion as shown in MTT assay, (3)H-thymidine incorporation and S-phase percentage up to 2-fold. Two subtypes of AFP receptors were identified in the cells with Kds of 1.3 x 10(-9)mol.L(-1) and 9.9 x10(-8)mol. (-1)L respectively. Pretreatment of cells with AFP resulted in a significant increase (625%) in cAMP accumulation. The activity of protein kinase A activity were increased up to 37.5, 122.6, 73.7 and 61.2% at treatment time point 2, 6, 12 and 24 hours. The level of intracellular calcium were elevated after the treatment of alpha-fetoprotein and achieved to 204% at 4 min. The results also showed that AFP(20mg.L(-1)) could upregulate the expression of N- ras oncogenes and p 53 and p21( ras ) in Bel 7402 cells. In the later case,the alteration were 81.1%(12h) and 97.3%(12h) respectively compared with control. CONCLUSION: These results demonstrate that AFP is a potential growth factor to promote the proliferation of human hepatoma Bel 7402 cells. Its growth-regulatory effects are mediated by its specific plasma membrane receptors coupled with its transmembrane signaling transduction through the pathway of cAMP-PKA and intracellular calcium to regulate the expression of oncogenes.

分维场论－分形理论的新拓展

分维场论－分形理论的新拓展周文臣（临沂师范学校．２７６００５，山东临沂；３３岁，男，高级讲师）在目前的分形理论中，无论是简单的分形维数，还是复杂的多标度分形维数，都是对事物演化过程中的暂态的描述，所研究的仅仅是作为演化结果的分形结构的几何性态，对于如...

Blockage of IGF-1R signaling sensitizes urinary bladder cancer cells to mitomycin-mediated cytotoxicity

A major problem which is poorly understood in the management of bladder cancer is low sensitivity to chemotherapy and high recurrence after transurethral resection. Insulin-like growth factor 1 receptor (IGF-1R) signaling plays a very important role in progression, invasion and metastasis of bladder cancer cells. In this study, we investigated whether IGF-1R was involved in the growth stimulating activity and drug resistance of bladder cancer cells. The results showed: The mRNAs of IGF-1, IGF-2 and IGF-1R were strongly expressed in serum-free cultured T24 cell line, whereas normal urothelial cells did not express these factors/receptors or only in trace levels; T24 cell responded far better to growth stimulation by IGF-1 than did normal urothelial cells; blockage of IGF1R by antisense oligodeoxynucleotide (ODN) significantly inhibited the growth of T24 cell and enhanced sensitivity and apoptosis of T24 cells to mitomycin (MMC). These results suggested that blockage of IGF-IR signaling might potentially contribute to the treatment of bladder cancer cells which are insensitive to chemotherapy.

Study on Root Morphology of Erect Type Peanut

The root morphology of erect type peanut in deep soil was studied in this paper. In the experiment, erect type peanut showed as most as five-order lateral roots with 13 227 pieces of lateral roots. At the seedling stage, the root system of erect type peanut was handstand cone-typed with lateral roots at various orders distributed around the taproot, and among the roots, the taproot was longest. During the late seedling stage to mature stage, the upper part of the root system of erect type peanut was blunt cone-typed, the middle part was three-dimensional network typed, while the lower part was cyUnder-like. The longest first-order lateral root was the longest root. At the mature stage, the taproot was not always the deepest root.

Effects of Sunitinib Malate on Growth of Human Bladder Transitional Cell Line T24 In Vitro

Objective To investigate the growth-inhibitory effect of sunitinib malate on human bladder transitional cell carcinoma （TCC） in vitro. Methods Human bladder TCC cell line T24 was cultured and exposed to graded concentrations of sunitinib malate for 72 hours in vitro to determine the sensitivities to drug. Cell viability was measured by MTT assay. Cell apoptotic morphology was observed by fluorescence microscope following DAPl staining. Band expressions of Fas, Fas ligand, poly （ADP-ribose） polyrnerase （PARP） and D-actin were analyzed by Western blot. Wound healing process of T24 cells exposed to sunitinib malate was assayed. Results Sunitinib malate exerted a concentration-dependent and time-dependent inhibitory effect on the T24 cell lines. Fluorescence microscopy showed that small vacuoles appeared in the nuclei of T24 cells and the vacuoles were bigger with higher drug concentrations. The expressions of Fas ligand and PARP in T24 cells treated with sunitinib malate exhibited a concentration-dependent increase. Moreover sunitinib malate suppressed the wound healing process in a concentration-dependent manner. Conclusion Sunitinib malate exerted marked inhibitory activity against bladder cancer cell line T24.

Expression of thymidine kinase mediated by a novel non-viral delivery system under the control of vascular endothelial growth factor receptor 2 promoter selectively kills human umbilical vein endothelial cells

AIM: To investigate the killing efficiency of a recombinant plasmid containing a thymidine kinase (TK) domain insert driven by the vascular endothelial growth factor receptor 2 (VEGFR2) promoter (KDR) on vascular endothelial cells.METHODS: The KDR-TK fragment was extracted from pBluescript Ⅱ KDR-TK plasmid by enzymatic digestion with Xho I and Sal I. The enhanced green fluorescence protein (EGFP) carrier was extracted from pEGFP by the same procedure. The KDR-TK was inserted into the pEGFP carrier to construct pEGFP-KDR-TK. Using ultrasound irradiation and microbubble, pEGFP-KDR-TK was transferred into human umbilical vein endothelial cells (HUVECs). The transient infection rate was estimated by green fluorescent protein (GFP) expression. Transfected HUVECs, non-transfected HUVECs, and HepG2 cells were cultured in the presence of different concentrations of ganciclovir (GCV), and the killing efficacy of HSV-TK/GCV was analyzed by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium bromide (MTT) assay. RESULTS: The recombinant pEGFP-KDR-TK was successfully constructed by inserting the KDR-TK fragment into the pEGFP carrier. Transfected HUVECs showed cytoplasmic green fluorescence, and the transient transfection rate was about 20.3%. Pools of G418-resistant cells exhibited a higher sensitivity to theprodrug/GCV compared to non-transfected HUVECs or non-transfected HepG2 cells, respectively. CONCLUSION: KDR promoter and the suicide gene/prodrug system mediated by diagnostic ultrasound combined with microbubble can significantly kill HUVECs. Such therapy may present a novel and attractive approach to target gene therapy on tumor vessels.

Numerical study on freezing-thawing phase change heat transfer in biological tissue embedded with two cryoprobes

A two-dimensional model for freezing and thawing phase change heat transfer in biological tissue embedded with two cryoprobes was established.In this model,the blood vessels were considered as tree-like branched fractal network,and the effective flow rate and effective thermal conductivity of blood were obtained by fractal method.The temperature distribution and ice crystal growth process in biological tissue embedded with two cryoprobes during freezing-thawing process were numerically simulated.The results show that the growth velocity of ice crystal in freezing process from 200 to 400 s is more rapid than that from 400 to 600 s. Thawing process of frozen tissue occurs in the regions around cryoprobes tips and tissue boundary simultaneously,and the phase interfaces are close to each other until ice crystal melts completely.The distance of two cryoprobes has a more profound effect on the temperature distribution in freezing process at 400 s than at 800 s.

ERK1/2 contributes negative regulation to STAT3 activity in HSS-transfected HepG2 cells

Signal transducer and activator of transcription 3 (STAT3) is a recently characterized transcription factor which is essential to liver regeneration. We have previously reported that hepatic stimulator substance (HSS), a novel growthpromoting substance, phosphorylated the epidermal growth factor (EGF) receptors and activated downstream RasMAP kinase (extracellular signal-regulated kinases, ERK1/2) cascade. However, whether HSS signal is related to STAT3pathway remains unclear. The present study is aiming to explore the regulatory effect of activation of ERK1/2 evoked by HSS on STAT3 phosphorylation and STAT3 signaling. Human hepatoma cell line HepG2 was stably transfected with HSS cDNA and HSS expression was measured by Northern blot. The results showed that the transfection of HSS into HepG2 resulted in remarkable increase in cellular proliferation as compared with the non-transfected cells, and it was further proved that the cellular proliferation in the HSS-transfected cells was related to ERK1/2 activation. Treatment of the cells with 50 μM of PD98059, an ERK1/2 specific upstream inhibitor, resulted in ERK1/2 inactivation completely.Inhibition of ERK1/2 allowed the tyrosine of STAT3 to be phosphorylated in a dose-dependent manner to PD98059.Furthermore, transient transfection of STAT3 mutant (STAT3S727A) into HSS-bearing cells could remarkably reverse the inhibitory effect of ERK1/2 on STAT3 phosphorylation. Based upon these results, it is concluded that ERK1/2negatively modulates STAT3 phosphorylation and this function is dependent on residual serine-727 (S727) of STAT3.

Effects of herpes simplex virus thymidine kinase/acyclovir system on growth of human pulmonary adenocarcinoma A549 cell line in vitro and in vivo

Objective: To observe the effect of anciclovir (ACV) treatment on tumors induced by inoculation of TK gene-transfected human pulmonary adenocarcinoma A549 cells in nude mice. Methods: A recombinant plasmid containing TK gene was constructed and transfected into A549 cells by electroporation. The sensitivity of the transgenic cells (A549-TK) to ACV was examined by MTT assay in vitro and for in vivo observation, inoculation of A549-TK and A-549 cells into nude mice was separately performed to induce tumor growth, the response of which to ACV treatment was observed, and the tumor tissues were pathologically examined. Results: A recombinant plasmid containing TK gene was successfully constructed and transfected into A549 cells. The sensitivity of A549-TK cells to ACV was 43 times higher than that of A549 cells. The tumors induced by A549-TK cells showed no significant increase in size after ACV treatment (P>0. 05) , and light microscopy revealed local tissue necrosis, karyoklasis, and nuclei disappearance. Conclusion: A549-TK cells acquires sensitivity to ACV both in vitro and in vivo, and ACV can inhibit the growth of tumors induced by A549-TK cell inoculation in nude mice.

In Vitro Propagation of Echeveria elegans, a Species of the Flora Endangered Mexican

The genus Echeveria has about 143 species that are unique to the Americas, 117 of which are presented in the Mexican Republic, mainly in the states of Hidalgo, Puebla and Oaxaca. Propagation can be done by cuttings, leaf cuttings or seeds, but these methods are insufficient to avoid the predation of many species. NOM-059-ECOL-2001 shows Echeveria elegans as species reported endangered. The objectives in this study were achieved in vitro propagation from axillary buds, as an alternative to multiplication and preservation species. We evaluated different concentrations and combinations of two growth regulators, 6-BAP （2.22, 4.44, 6.66 and 8.88 μM） and u-NAA （1.35 and 2.70 μM） on shoot formation from axillary buds and two culture media with different concentrations of MS salts, to achieve root plants grown in vitro. It was found by combining 6.66 μM of 6-BAP and 1.35μM of α-NAA favored the formation and development of new shoots. In the rooting stage, the best results are achieved with the treatment containing 50% of MS salts. Then the plants were transplanted into greenhouses and achieved a successful acclimatization. During this last phase of development, there were no phenotypic changes or presence of somaclonal variants.

The ＂Minister of Production in the Collectivist State＂ as a Third Way of the Market： China Between Ethics and Growth

This paper proposes a comparison between the Chinese social-economic system of today and the economic planning system theorized by Enrico Barone in 1908, which described in his famous paper ＂The minister of production in the collectivist state＂. The work stems from a critical reflection on the premises of the capitalist market system. From Adam Smith to J. M. Keynes （included）, economists have identified in traditional capitalist models （search for maximum profit, economic efficiency, free competition system） the best economic system, universally implementable, feasible and infallible in democratic capitalist contexts. In the history of economic ideas have always been clear gap between the ＂capitalist system＂ and the ＂collectivist system＂. The choice of a government had to fall back on one or on the other system. The first considered ＂good＂, the second considered ＂bad＂ for economic growth. Times since September 11, 2001, however, seem to have debunked the myth of capitalism as the only model of democratic growth： Asia and China have given the world a lesson in humility. China has shown that although not being a democratic country could developed a robust and highly competitive economic model that has weakened the pillars of the western capitalism. China through Confucianism and Taoism has been able to establish the new global economic laws： low labour costs, low prices, mass production of low quality with a mixed mercantilist philosophy which is unknown to the Western world： the silent, smooth, radical trade expansion. As in a game of dominoes, China has been able to drop the capitalism safeties and America had to bow its head and agree to no longer be the only great power in the international scenario. But the peculiarity of this work is not only to define the root causes of China＇s success in the West; also reflects the fact that an Italian economist （Enrico Barone）, in 1908, predicted analytically and developed a theoretical system very similar to that of China nowadays, between capitalism and collectivism （a third way of the market）. The Asian culture has developed a market system that has proven to be accommodating to the needs of the capitalist market and to grasp the development opportunities that the same Western capitalism has offered.

The use of suicide gene systems in vascular cells in vitro

To investigate the efficiency of suicide gene systems on vascular cells, HSV-tk/GCV and EC-CD/5-FC systems were established on vascular endothelial cells in vitro by retroviral transduction. Both modified cell lines were highly sensitive to prodrugs, the IC50 for GCV was less than 0.4 μM, and IC50 for 5-FC was less than 75 μM,while the parental endothelial cells were insensitive even at the highest concentrations of prodrugs in this experiment. Mixed cellular assay showed that significant bystander effect was exhibited in modified endothelial cells.When only 10% or 30% of the mixed cells were tk positive and exposed to 20 μM GCV for 6 days, more than 60% or 90% of the whole population was killed. Similar result was also found in CD positive cells. These results indicated that both HSV-tk/GCV and EC-CD/5-FC systems could efficiently suppress endothelial cell growth in vitro.

史论教学的回望与审思——《外国建筑史》课程教学的几点感悟

本文通过剖析当今时代建筑史教学的难点与重点,以参加“2018年建筑史教学观摩交流会”为起点,融合其后4年间的一线史论课程教学经历,结合《外国建筑史》中“文艺复兴的开端——佛罗伦萨大教堂穹顶”为具体课程案例,尝试以学科交叉、情景再现、历史“穿越”等代入式“回望”的教学方法,引导学生去自主审思一位建筑师、一座建筑、一个时代,促使学习思维与讲授内容的深入互动。笔者希望通过对现时代建筑史教学的定位和理解,以美育为核心,传承建筑史教学传统,建构更全面的教学“生长体系”,并能紧跟时代浪潮,给予学生更多的人文性、交融性、体验性的教学关怀,更好地促进建筑史教学发展。

One-pot synthesis of Bi2Se3 nanostructures with rationally tunable morphologies

Shape control has proven to be a powerful and versatile means of tailoring the properties of Bi2Se3 nanostructures for a wide variety of applications. Here, three different Bi2Se3 nanostructures, i.e., spiral-type nanoplates, smooth nanoplates, and dendritic nanostructures, were prepared by manipulating the supersaturation level in the synthetic system. This mechanism study indicated that, at low supersaturation, defects in the crystal growth could cause a step edge upon which Bi2Se3 particles were added continuously, leading to the formation of spiral-type nanoplates. At intermediate supersaturation, the aggregation of amorphous Bi2Se3 particles and subsequent recrystallization resulted in the formation of smooth nanoplates. Furthermore, at high supersaturation, polycrystalline Bi2Se3 cores formed initially, on which anisotropic growth of Bi2Se3 occurred. This work not only advances our understanding of the growth mechanism but also offers a new approach to control the morphology of Bi2Se3 nanostructures.

p75NTR signal transduction suppressed by BFAR and p75NTR interactions

p75NTR is a low-affinity nerve growth factor receptor, which promotes cell proliferation as a positive modulator of high-affinity receptor TrkA, as well as binds with cell ligands to induce apoptosis and mediate death signals. To analyze the regulatory mechanisms of p75NTR, the present study utilized a new membrane yeast two-hybrid system to screen a human fe- tal brain cDNA library. Results identified BFAR, a novel protein that interacts with p75NTR. Interaction specificity was veri- fied by membrane yeast two-hybrid co-transformation assays, in vitro GST pull-down assays, and in vitro co-irnmunopreci- pitation assays. The fluorescent subcellular localization assay revealed that the two proteins co-localized within the cytoplasm. BFAR overexpression in PC-12 and HEK293T cells inhibited the NFnB and JNK signaling pathway, as determined with the luciferase test. Co-transfected p75NTR and BFAR in HEK293T or PC-12 cells, respectively, increased the percentage of cells in the G2/M phase, decreased the number of S-phase cells, and did not change the number of G0/Gl-phase cells.