目前,心血管疾病如动脉粥样硬化、心肌梗死、心脏肥大和心力衰竭等是全球主要的死亡原因,严重危害人类的生命安全,因此,心血管疾病的早期诊断、预后监测及治疗至关重要。近年来,生物标志物在心血管疾病患者的诊断和预后评估中具有越来...目前,心血管疾病如动脉粥样硬化、心肌梗死、心脏肥大和心力衰竭等是全球主要的死亡原因,严重危害人类的生命安全,因此,心血管疾病的早期诊断、预后监测及治疗至关重要。近年来,生物标志物在心血管疾病患者的诊断和预后评估中具有越来越重要的临床价值。其中,生长分化因子15 (growth differentiation factor 15, GDF15)是一种新兴的心血管疾病生物标志物,是死亡率和不良预后的独立预测因子。许多基础研究还表明,GDF15在心血管疾病中发挥保护作用,可能具有潜在的治疗价值。本文就GDF15在心血管疾病中的诊疗作用及相关研究进展做一综述,旨在探索GDF15在心血管疾病诊断、预后监测和治疗中的应用前景。Currently, cardiovascular diseases (CVDs) such as atherosclerosis, myocardial infarction, cardiac hypertrophy, and heart failure are the leading causes of death globally, posing a serious threat to human life safety. Therefore, early diagnosis, prognosis monitoring, and treatment of CVDs are crucial. In recent years, biomarkers have become increasingly important in the diagnosis and prognosis assessment of patients with CVDs. Among them, growth differentiation factor 15 (GDF15) is an emerging biomarker for CVDs and an independent predictor of mortality and poor prognosis. Many basic research studies have also indicated that GDF15 plays a protective role in CVDs and may have potential therapeutic value. This article reviews the diagnostic and therapeutic roles of GDF15 in CVDs and related research progress, aiming to explore the application prospects of GDF15 in the diagnosis, prognosis monitoring, and treatment of CVDs.展开更多
目的:探讨生长分化因子15对心肌梗死大鼠的作用。方法:18只SPF级雄性SD大鼠根据随机数字表法分为模型组、实验组(A组GDF-15低剂量组10 ng/mL、B组GDF-15高剂量组50 ng/mL),每组6只,适应性喂养1周后,每天给药1次,连续1周,末次给药6 h后,...目的:探讨生长分化因子15对心肌梗死大鼠的作用。方法:18只SPF级雄性SD大鼠根据随机数字表法分为模型组、实验组(A组GDF-15低剂量组10 ng/mL、B组GDF-15高剂量组50 ng/mL),每组6只,适应性喂养1周后,每天给药1次,连续1周,末次给药6 h后,各组大鼠均结扎冠状动脉左前降支进行心肌梗死造模。采用TUNEL/DAPI双染色法观察大鼠心肌细胞凋亡情况,分别统计凋亡细胞数及细胞总数,计算出细胞凋亡率。结果:与模型组相比,对照组B的细胞凋亡率显著减少(P Objective: To investigate the effect of growth differentiation factor 15 on myocardial infarction in rats. Methods: 18 SPF grade male SD rats were divided into model group and experimental group according to random number table method (group A, low-dose GDF-15 group, 10 ng/mL, group B, high-dose GDF-15 group, 50 ng/mL), with 6 rats in each group. After adaptive feeding for 1 week, the rats were given the drug once a day for 1 week. The left anterior descending branch of the coronary artery was lapped in each group for myocardial infarction modeling. TUNEL/DAPI double staining was used to observe the apoptosis of rat cardiomyocytes. The number of apoptotic cells and the total number of apoptotic cells were counted, and the apoptosis rate was calculated. Results: Compared with model group, the apoptosis rate of control group B was significantly decreased (P < 0.01), and GDF-15 reduced the apoptosis rate in a dose-dependent manner (P < 0.05). Conclusion: Growth differentiation factor 15 can reduce apoptosis in rats with myocardial infarction.展开更多
文摘目前,心血管疾病如动脉粥样硬化、心肌梗死、心脏肥大和心力衰竭等是全球主要的死亡原因,严重危害人类的生命安全,因此,心血管疾病的早期诊断、预后监测及治疗至关重要。近年来,生物标志物在心血管疾病患者的诊断和预后评估中具有越来越重要的临床价值。其中,生长分化因子15 (growth differentiation factor 15, GDF15)是一种新兴的心血管疾病生物标志物,是死亡率和不良预后的独立预测因子。许多基础研究还表明,GDF15在心血管疾病中发挥保护作用,可能具有潜在的治疗价值。本文就GDF15在心血管疾病中的诊疗作用及相关研究进展做一综述,旨在探索GDF15在心血管疾病诊断、预后监测和治疗中的应用前景。Currently, cardiovascular diseases (CVDs) such as atherosclerosis, myocardial infarction, cardiac hypertrophy, and heart failure are the leading causes of death globally, posing a serious threat to human life safety. Therefore, early diagnosis, prognosis monitoring, and treatment of CVDs are crucial. In recent years, biomarkers have become increasingly important in the diagnosis and prognosis assessment of patients with CVDs. Among them, growth differentiation factor 15 (GDF15) is an emerging biomarker for CVDs and an independent predictor of mortality and poor prognosis. Many basic research studies have also indicated that GDF15 plays a protective role in CVDs and may have potential therapeutic value. This article reviews the diagnostic and therapeutic roles of GDF15 in CVDs and related research progress, aiming to explore the application prospects of GDF15 in the diagnosis, prognosis monitoring, and treatment of CVDs.
文摘目的:探讨生长分化因子15对心肌梗死大鼠的作用。方法:18只SPF级雄性SD大鼠根据随机数字表法分为模型组、实验组(A组GDF-15低剂量组10 ng/mL、B组GDF-15高剂量组50 ng/mL),每组6只,适应性喂养1周后,每天给药1次,连续1周,末次给药6 h后,各组大鼠均结扎冠状动脉左前降支进行心肌梗死造模。采用TUNEL/DAPI双染色法观察大鼠心肌细胞凋亡情况,分别统计凋亡细胞数及细胞总数,计算出细胞凋亡率。结果:与模型组相比,对照组B的细胞凋亡率显著减少(P Objective: To investigate the effect of growth differentiation factor 15 on myocardial infarction in rats. Methods: 18 SPF grade male SD rats were divided into model group and experimental group according to random number table method (group A, low-dose GDF-15 group, 10 ng/mL, group B, high-dose GDF-15 group, 50 ng/mL), with 6 rats in each group. After adaptive feeding for 1 week, the rats were given the drug once a day for 1 week. The left anterior descending branch of the coronary artery was lapped in each group for myocardial infarction modeling. TUNEL/DAPI double staining was used to observe the apoptosis of rat cardiomyocytes. The number of apoptotic cells and the total number of apoptotic cells were counted, and the apoptosis rate was calculated. Results: Compared with model group, the apoptosis rate of control group B was significantly decreased (P < 0.01), and GDF-15 reduced the apoptosis rate in a dose-dependent manner (P < 0.05). Conclusion: Growth differentiation factor 15 can reduce apoptosis in rats with myocardial infarction.
