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Hepatic reconstruction from fetal porcine liver cells using a radial flow bioreactor 被引量:3
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作者 Yuji Ishii Ryota Saito +3 位作者 Hideki Marushima Ryusuke Ito Taro Sakamoto Katsuhiko Yanaga 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第17期2740-2747,共8页
AIM: To examine the efficacy of the radial flow bioreactor (RFB) as an extracorporeal bioartificial liver (BAL) and the reconstruction of liver organoids using embryonic pig liver cells. METHODS: We reconstructe... AIM: To examine the efficacy of the radial flow bioreactor (RFB) as an extracorporeal bioartificial liver (BAL) and the reconstruction of liver organoids using embryonic pig liver cells. METHODS: We reconstructed the liver organoids using embryonic porcine liver cells in the RFB. We also determined the gestational time window for the optimum growth of embryonic porcine liver cells. Five weeks of gestation was designated as embryonic day (E) 35 and 8 wk of gestation was designated as E56. These cells were cultured for one week before morphological and functional examinations. Moreover, the efficacy of pulsed adminisbation of a high concentration hepatocyte growth factor (HGF) was examined. RESULTS: Both cell growth and function were excellent after harvesting on E35. The pulsed administration of a high concentration of HGF promoted the differentiation and maturation of these fetal hepatic cells. Microscopic examination of organoids in the RFB revealed palisading and showed that bile duct-like structures were well developed, indicating that the organoids were mini livers. Transmission electron microscopy revealed microvilli on the luminal surfaces of bile duct-like structures and junctional complexes, which form the basis of the cytoskeleton of epithelial tissues. Furthermore, strong expression of connexin (Cx) 32, which is the main protein of hepatocyte gap junctions, was observed. With respect to liver function, ammonia detoxification and urea synthesis were shown to be performed effectively. CONCLUSION: Our system can potentially be applied in the fields of BAL and transplantation medicine. 展开更多
关键词 Liver organoid Radial flow bioreactor Bioartificial liver Fetal porcine liver Hepatocyte growthfactor
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Reactivation of the insulin-like growth factor-Ⅱsignaling pathway in human hepatocellular carcinoma 被引量:40
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作者 Kai Breuhahn Peter Schirmacher 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第11期1690-1698,共9页
Constitutive activation of the insulin-like growth factor (IGF)-signaling axis is frequently observed in human hepatocellular carcinoma(HCC).Especially the over- expression of the fetal growth factor IGF-Ⅱ,IGF-Ⅰ rec... Constitutive activation of the insulin-like growth factor (IGF)-signaling axis is frequently observed in human hepatocellular carcinoma(HCC).Especially the over- expression of the fetal growth factor IGF-Ⅱ,IGF-Ⅰ receptor(IGF-IR),and cytoplasmic downstream effectors such as insulin-receptor substrates(IRS)contribute to proliferation,anti-apoptosis,and invasive behavior. This review focuses on the relevant alterations in this signaling pathway and independent in vivo models that support the central role IGF-Ⅱsignaling during HCC development and progression.Since this pathway has become the center of interest as a target for potential anti-cancer therapy in many types of malignancies,various experimental strategies have been developed,including neutralizing antibodies and selective receptor kinase inhibitors,with respect to the specific and efficient reduction of oncogenic IGF-Ⅱ/IGF-IR-signaling. 展开更多
关键词 Hepatocellular carcinoma Insulin-like growth factor-Ⅱ Insulin-like growth factor-Ⅰ receptor Insulin receptor substrate House models THERAPY
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背驮式同种原位肝移植术1例报告
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作者 彭民浩 杨定华 +9 位作者 卢榜裕 李绍森 陈希纲 黄源 林坚 肖开银 卢金书 冯穗生 王文光 刘家奇 《肝胆胰脾外科杂志》 CSCD 1997年第2期78-81,共4页
对1例患原发性胆汁性肝硬变台并门脉高压症终来期病人作了背驮式同种原位肝移植术,手术获得成功,术后恢复良好。认为该术式由于保留了受体的肝后下腔静脉,具有术中血流动力学稳定,肾功能不受影响,术后恢复快等优点,因此可以作为... 对1例患原发性胆汁性肝硬变台并门脉高压症终来期病人作了背驮式同种原位肝移植术,手术获得成功,术后恢复良好。认为该术式由于保留了受体的肝后下腔静脉,具有术中血流动力学稳定,肾功能不受影响,术后恢复快等优点,因此可以作为肝移植的常规术式。但是对术前一般情况差或者估计无肝期时间长的病人,采用背驮式手术时应加作门静脉-腋静脉(成人颈静脉)转流。作者对Starzl介绍的吻合肝动脉的生长因素法(growtb facfor)的改良,不但适用于小动脉吻合,也适用于静脉吻台,可以避免或减少吻合血管的狭窄和血栓形成。在胆管重建时强调保证胆管血供的重要性并且放弃了胆总管的T管引流法,改用经胆囊管插管引流,可以减少胆总管的损伤和术后胆管狭窄的机会。 展开更多
关键词 背驮式同种原位肝移植术 病例报告 生长因素法 动脉吻合 T管引流
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Effect of Acupuncture-moxibustion on the Expression of IGF-1 and SOCS2 in Colonic Mucosa of Rats with Ulcerative Colitis 被引量:4
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作者 王晓梅 关鑫 +2 位作者 季光 施征 吴焕淦 《Journal of Acupuncture and Tuina Science》 2010年第4期204-209,共6页
Objective: To observe the effect of acupuncture-moxibustion on the expression of insulin-like growth factor-I (IGF-I) and suppressor of cytokine signaling-2 (SOCS2) in colonic mucosa of rat models of ulcerative... Objective: To observe the effect of acupuncture-moxibustion on the expression of insulin-like growth factor-I (IGF-I) and suppressor of cytokine signaling-2 (SOCS2) in colonic mucosa of rat models of ulcerative colitis (UC), and explore the mechanism of acupuncture- moxibustion therapy in treating UC. Methods: The rats were randomized into a normal control (NC) group, a model control (MC) group, an herb-partitioned moxibustion (HPM) group and an electroacupuncture (EA) group, 8 in each group. The rat models of UC were established by immunological methods combined with local stimulation. The rats in the HPM and EA groups were given herb-partitioned moxibustion and electroacupuncture treatments respectively, once every day, lasting for 14 d. The morphological variations of rat's colonic mucosa were observed under light microscope; the colonic mucosal mucin was detected by PAS-AB and HID-AB staining methods; the expression of IGF-1 and SOCS2 was assayed by the immunohistochemical method. Results: In the rat models of UC, ulceration and inflammation of the colon were revealed by light microscope. The concentration of colonic mucosal mucin was reduced (P〈0.01), while the expression of IGF-1 had an increase (P〈0.01), and the expression of SOCS2 was reduced (P〈0.01). After HPM or EA treatment, the pathological injuries of colonic mucosa had improved, the concentration of mucin increased (P〈0.01), the expression of IGF-1 decreased (P〈0.01), and the expression of SOCS2 increased (P〈0.01). Conclusion: The secretion of mucosal mucin in rat UC decreased, the expression of IGF-1 was significantly higher, while the expression of SOCS2 was remarkably lower; both HPM and EA can help improve the damage of colonic mucosa in rat UC, and modulate the secretion of mucin, as well as regulate the expression of IGF-1 and SOCS2 in the colonic mucosa. 展开更多
关键词 Colitis Ulcerative Acupuncture-moxibustion Therapy Insulin-Like Growth Factor I Suppressor of Cytokine Signaling Proteins
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