藏东南高寒森林大型真菌物种增补及生态特点

对西藏东南高寒森林地区2年内较为系统的调查,研究了大型真菌物种多样性及其生态特点。采集获得大型真菌标本1100余份,通过分类学鉴定为2门4纲16目44科118属239种,其中:担子菌门1纲11目34科106属222种,子囊菌门3纲5目10科12属17种,22种为西藏新记录种。从营养方式上看,腐生真菌的比例为39.38%,菌根真菌的比例为60.18%;从生长基物上看,营土壤生活的物种为63.00%,营木质物生活的约为34.80%,营枯落物生活的约为1.76%,在菇体上生长的约为0.44%。大型真菌物种发生与降水变化具有相关性。

贵州鸡油菌的应用与生物学特性的研究

为了进一步开发利用贵州鸡油菌资源 ,本文在对贵州鸡油菌资源进行调查的同时 ,对其生物学特性及生长基物作了初步的研究。其结果为 :鸡油菌分布在贵州海拔 2 50～ 1750的地区。子实体生长期为每年的 5～8月 ,生长基物的 pH为 5 8,生长温度为 15 2～ 30 6℃ ,光照强度为 650～ 1650LX ,土壤温度 31 39%～19 4 4 % (土壤深度 1～ 10cm) ,土壤微量元素有 :铝、钡、铍、钴、铬、铜、铁、锰、镍、铅、锑、硅、钛、矾、铟。鸡油菌生长环境的植物种类有 :茅栗、栓皮栎、马尾松、马桑、盐夫木、板栗、化香、槐树、野花椒、地瓜藤、构皮、救军粮。将分离的鸡油菌纯菌种接种于马尾松育苗中 ,能形成菌根 。

贵州冬小包脚菇生物学特性及复合生态

在对冬小包脚菇子实体及生长基物的无机成份进行测定的同时 ,对其子实体的营养成份 (即粗蛋白、氨基酸、还原糖等 )、生长基物 (农作物桔杆稻桩 )的分解情况、土壤有机质含量进行了分析测定。在研究样方的生态观测中 ,测定了光照强度、气温、地温、降水量 ,土壤酸碱度、容重、比重、孔隙度、自然含水量等。

中国盘菌属Peziza一新记录种

盘菌属Peziza Fr.隶属于真菌界 Fungi、子囊菌门 Ascomycota、子囊菌纲 Ascomycetes、盘菌目 Pezizales、盘菌科 Pezizaceae,其子实体大至小型,外形为盘状、杯状至钵状,颜色多样,尤以深色者居多,生长基物各异.

Effect of thermal stabilization on microstructure and mechanical property of directionally solidified Ti-46Al-0.5W-0.5Si alloy

Effect of thermal stabilization on the microstructure and mechanical property of directionally solidified Ti-46Al-0.5W-0.5Si （mole fraction, %） alloy was investigated. The specimens were thermal stabilized for different time （t） and directionally solidified at a constant growth rate of 30 μm/s and temperature gradient of 20 K/mm. Dependencies of the primary dendritic spacing （λ1）, secondary dendritic spacing （λ2）, interlamellar spacing （λL） and microhardness （HV） on holding time were determined. The values of the λ1, λ2 and λL increase with the increase of t, and the value of HV decreases with the increase of t. The increase of t is helpful to obtain a good directional solidification structure. However, it reduces the mechanical property of the directionally solidified TiAl alloy. The optimized value of t is about 30 min.

Metallothionein:An overview

Metallothioneins （MTs） were discovered in 1957 by Margoshes and Vallee and identified as low-molecular weight and sulphydryl rich proteins. It is not surprising that most mammalian tissues contain age related basal levels of MTs since they are involved in metalloregulatory processes that include cell growth and multiplication. In an effort to understand the biology of this intriguing tumor, various biomarkers such as oncogenes, p53 tumor suppressor gene, war 1 protein, proliferating cell nuclear antigen, telomerase, microsatellite markers and cytogenetic changes have been examined. One biomarker which has recently shown to be expressed in various human tumors but still less reported in carcinoma is MT. Immunohistochemical detection of MT proteins in cold acetone-fixed paraffin embedded liver sections was performed by the streptavidin-avidin-biotin immunoperoxidase complex method.

Performance of Tree Species Growing on Tailings Dam Soils in Zambia： A Basis for Selection of Species for Re-vegetating Tailings Dams

To facilitate the development of the basis for the selection of plants for re-vegetating tailings in Zambia, an as sessment of the performance of tree species growing on tailings dams was conducted. The performance of species was determined in terms of relative density, frequency and importance value for each species. The study reveals variations in performance of species; for example in terms of importance values, Acacia polyacantha （33.5%）, Toona ciliata （21.4%）, Acacia sieberana （9.9%）, Bauhinia thonningii （9.1%） and Peltophorum africanum （8.3%） were the most dominant species. The dominance of these species on tailings dams demonstrates tolerance to railings dams conditions. The study recommends that emphasis must be placed on these species in re-vegetating tailings dams.

Inoculation with Pseudomonas spp. Containing ACC-Deaminase Partially Eliminates the Effects of Drought Stress on Growth,Yield,and Ripening of Pea (Pisum sativum L.)

Two preselected plant growth promoting rhizobacteria(PGPR) containing 1-aminocyclopropane-1-carboxylate(ACC) -deaminase(EC 4.1.99.4) were used to investigate their potential to ameliorate the effects of drought stress on growth,yield,and ripening of pea(Pisum sativum L.) . Inoculated and uninoculated(control) seeds of pea cultivar 2000 were sown in pots(four seeds pot-1) and placed in a wire house. The plants were exposed to drought stress at different stages of growth(vegetative,flowering,and pod formation) by skipping the respective irrigation. Results revealed that inoculation of peas with PGPR containing ACC-deaminase significantly decreased the "drought stress imposed effects" on the growth and yield of peas. Exposure of plants to drought stress at vegetative growth stage significantly decreased shoot growth by 41% in the case of uninoculated plants,whereas,by only 18% in the case of inoculated plants compared to nonstressed uninoculated control. Grain yield was decreased when plants were exposed to drought stress at the flowering and pod formation stage,but inoculation resulted in better grain yield(up to 62% and 40% higher,respectively) than the respective uninoculated nonstressed control. Ripening of pods was also delayed in plants inoculated with PGPR,which may imply decreased endogenous ethylene production in inoculated plants. This premise is further supported by the observation that inoculation with PGPR reduced the intensity of classical "triple" response in etiolated pea seedlings,caused by externally applied ACC. It is very probable that the drought stress induced inhibitory effects of ethylene could be partially or completely eliminated by inoculation with PGPR containing ACC-deaminase.

MicroRNAs and cancer

MicroRNAs (miRNAs) belong to a class of noncoding, regulatory RNAs that are involved in oncogenesis and show remarkable tissue specificity.miRNAs are approximately 22 nt non-coding RNAs, which regulate gene expression in a sequence-specific manner via translational inhibition or messenger RNA (mRNA) degradation, thus affecting various cellular processes.Since the discovery of their fundamental mechanisms of action, the field of miRNAs has opened a new era in the understanding of small noncoding RNAs.Recent evidence has shown that miRNA controls cell growth, apoptosis, and differentiation.Cancer is a complex genetic disease caused by abnormalities in gene structure and expression, moreover, miRNA expression correlates with cancers and could have a crucial function in tumor progression.Bioinformatic data indicate that each miRNA can control hundreds of target genes, but identification of the accurate miRNA targets will be crucial to exploit the emerging knowledge of miRNA contribution to cancer process.

A novel long-chain acyl-derivative of epigallocatechin-3-O-gallate prepared and purified from green tea polyphenols

Lipophilic tea polyphenols (LTP) were prepared by catalytic esterification of green tea polyphenols (GTP) with hexadecanoyl chloride. A novel long chain acyl derivative of epigallocatechin 3 o gallate (EGCG) was first isolated from purification of LTP by high speed countercurrent chromatography (HSCCC) using a solvent system composed of n hexane ethyl acetate methanol water (1:1:1:1, v/v). The molecular structure of the acyl derivative, Epigallocatechin 3 O gallate 4′ O hexadecanate , was elucidated by means of elemental analysis, IR, 1H NMR and MS spectra.

Phylogenetic relationships within the genus Aspidisca(Protozoa,Ciliophora,Euplotida) revealed by ITS1-5.8S-ITS2 region sequences

Abstract The internal transcribed spacer regions （ITS1 and ITS2） and 5.8S rRNA genes were sequenced in six populations of four Aspidisca species, namely A. leptaspis, A. orthopogon, A. rnagna and A. aculeata. Phylogenetic trees were constructed by means of Bayesian inference （BI）, Maximum Parsimony （MP）, Neighbor-Joining （NJ）, and Maximum Likelihood （ML） to assess the inter- and intra-species relationships within the genus Aspidisca. All trees show similar topologies with stable supports and indicate that： （1） four well known groups, i.e., Oligotrichia, Stichotrichia, Choreotrichia and Hypotrichia, are distinctly outlined within the class Spirotrichea, and all are monophyletic other than Hypotrichia; （2） members of Aspidisca can be distinguished well, based on the ITSI-5.SS-ITS2 region sequences, and A. leptaspis and A. magna shared a closer relationship than other species; （3） Aspidisca and Euplotes branch early in the subclass Hypotrichia. To compare the phylogenetic relationships based on different genes, SSU rRNA trees were also constructed with nearly the same species inclusion, which revealed different topologies of inter-species, inter-genera and inter-subclasses.

Effects of irradiated Ergosan on the growth performance and mucus biological components of rainbow trout Oncorhynchus mykiss

Effects of irradiated and non-irradiated Ergosan extract(alginic acid) on rainbow trout growth performance and skin mucosal immunity were compared. Ergosan was irradiated at 30 kGy in a cobalt-60 irradiator. A total of 252 fish(128.03±9.4 g) were randomly divided into four equal groups,given the basal diet either unsupplemented with Ergosan(control group) or supplemented with crude Ergosan(5 g/kg),ethanol-extracted Ergosan(0.33 g/kg) or irradiated Ergosan(0.33 g/kg) according to this protocol: basal diet for 15 days,treatment diet for 15 days,basal diet for 10 days and treatment diet for 15 days. Highest growth performance was observed in fish fed irradiated Ergosan(P<0.05). Dietary administration of different Ergosan types did not cause any changes in mucus protein level,but improved alkaline phosphatase level and hemagglutination titer compared with the control(basal diet without Ergosan) on day 55 offeeding trial(P<0.05). Furthermore,the highest value of lysozyme activity was observed in gamma-irradiated Ergosan on day 55. In conclusion,gamma-irradiated Ergosan at 0.33 g/kg was found to improve growth performance and mucus biological components significantly in comparison with the control group(basal diet without Ergosan).

Influence of NaCI and Na2SO4 Treatments on Growth Development of Broad Bean （Vicia Faba, L.） Plant

Salinity treatments （NaCl and Na2SO4） reduced plant growth and increased the accumulation of Na^＋, P^3＋,Fe^3＋ , Mn^2＋, and CIin root, stem, leaf and legume. The uptake of K^＋ reduced in the presence of both salts whereas uptake of Ca^2＋ retarded mainly by Na2SO4. Chlorophyll content was affected mainly by NaCl, while Na2SO4 treatment lowered the rate of photosynthetic activity. Both salt compounds caused accumulation of free amino acids including free proline but reduced the protein content in the leaves. The salt tolerance capacity of the broad beans plant in which the observations of the effect of salinity determined by different concentrations of NaCI and Na2SO4 on growth, chloroplast pigments, photosynthetic, free amino acids, free proline, protein and inorganic elements on plants are combined with observations on growth and yield, in order to arrive at a better understanding of their salt tolerance, has been studied and discussed in this paper. The results for the latter only （fruiting stage） are presented.

Associations Between Epidermal Growth Factor Receptor Gene Mutation and Serum Tumor Markers in Advanced Lung Adenocarcinomas: A Retrospective Study

Objective To investigate the associations between epidermal growth factor receptor （EGFR） gene mutations and serum tumor markers in advanced lung adenocarcinomas. Methods We investigated the association between EGFR gene mutations and clinical features, including serum tumor marker levels, in 97 advanced lung adenocarcinomas patients who did not undergo the treatment of EGlaR tyrosine kinase inhibitors. EGFR gene mutation was detected by real-time PCR at exons 18, 19, 20, and 21. Serum tumor marker concentrations were analyzed by chemiluminescence assay kit at the same time. Results EGFR gene mutations were detected in 42 （43%） advanced lung adenocarcinoma patients. Gender （P=0.003）, smoking status （P=0.001）, and abnormal serum status of carcinoembryonic antigen （CEA, P=0.028） were significantly associated with EGFR gene mutation incidence. Multivariate analysis showed the abnormal CEA level in serum was independently associated with the incidence of EGFR gene mutation （P=0.046） with an odds ratio of 2.613 （95% Ch 1.018-6.710）. However, receiver operating characteristic （ROC） curve analysis revealed CEA was not an ideal predictive marker for EGFR gene mutation status in advanced lung adenocarcinoma （the area under the ROC curve was 0.608, P=0.069）. Conclusions EGFR gene mutation status is significantly associated with serum CEA status in advanced lung adenocarcinmoas. However, serum CEA is not an ideal predictor for EGFR mutation.

Isolation and functional analysis of a Brassica juncea gene encoding a com-ponent of auxin efflux carrier

Polar auxin transport plays a divergent role in plant growth and developmental processes including root and embryo development, vascular pattern formation and cell elongation. Recently isolated Arabidopsis pin gene family was believed to encode a component of auxin efflux carrier (G(?)lweiler et al, 1998). Based on the Arabidopsis pin1 sequence we have isolated a Brassica juncea cDNA (designated Bjpin1), which encoded a 70-kDa putative auxin efflux carrier. Deduced BjPIN1 shared 65% identities at protein level with AtPINl and was highly homologous to other putative PIN proteins of Arabidopsis (with highest homology to AtPIN3). Hydrophobic analysis showed similar structures between BjPINl and AtPIN proteins. Presence of 6 exons (varying in size between 65 bp and 1229 bp) and 5 introns (sizes between 89 bp and 463 bp) in the genomic fragment was revealed by comparing the genomic and cDNA sequences. Northern blot analysis indicated that Bjpin1 was expressed in most of the tissues tested, with a relatively higher level of transcript in flowers and a lower level in root tissues. Promoter-reporter gene fusion studies further revealed the expression of Bjpin1 in the mature pollen grains, young seeds, root tip, leaf vascular tissue and trace bundle, stem epidermis, cortex and vascular cells. BjPINl was localized on the plasma membrane as demonstrated through fusion expression of green fluorescent protein (GFP). Auxin efflux carrier activity was elevated in transgenic Arabidopsis expressing BjPIN1.

microRNAs regulation and its role as biomarkers in diseases

MicroRNAs （miRNAs）, approximately 21 to 23 nucleotides （nt） in length, belong to a set of smal non-coding RNA molecules that were not thought to be functional until the recent decades. miRNAs play important roles in many diseases such as various kinds of cancers and immune disorders. Many studies have focused on the relationship between miRNAs and diseases. miRNAs are significant mediators in human growth and development and in the genesis and development of diseases. Almost 30% of the activity of protein-coding genes is forecasted to be regulated by miRNAs in mammals, and some miRNAs are regarded as potential therapeutic targets for various diseases. In this review, we outline some functions of miRNAs, especialy those related to diseases.

Effects of Different Substrate Composition on Growth of Gesneriaceae Plants

The cultivation experiment was carried out to investigate the effects of dif-ferent proportions of peat soil, perlite, vermiculite and yel ow mud on growth of Gesneriaceae species （Chirita gueilinensis, Sinningia speciosa, Lysionotus pauci-florus, Hemiboea henryi, Aeschynanthus acuminatus, Saintpaulia ionantha）. The growth traits of each plant growing in 7 different matrix materials were investigated. The plant height, crown width and chlorophyl content of each plant were mea-sured. The results showed that the best substrate ratio was peat soil∶vermiculite=2∶1 for C. gueilinensis, L. pauciflorus and H. henryi; peat soil∶perlite∶vermiculite = 2∶1∶1 for S. ionantha; peat soil∶vermiculite∶yel ow mud=2∶1∶1 for S. speciosa; peat soil∶per-lite∶vermiculite∶yel ow mud=2∶1∶1∶1 for A. acuminatus.

Influence of Ectomycorrhizal Fungi on Absorption and Balance of Essential Elements of Pinus tabulaeformis Seedlings in Saline Soil

Pinus tabulaeformis seedlings were grown under a factorial design to measure biomass accumulation and P, Na and K concentrations of plants, with 3 different ectomycorrhizal fungus (EMF) strains (Boletus edulis, Xerocomus chrysenteron and Gomphidius viscidus) and two P treatments (with or without extra P). Growth and mineral nutrition of pine seedlings were stimulated by three EMF under salinity stress. Na concentrations were changed and plant K and P concentrations were increased significantly by EMF. Adding extra P made no difference in accumulation of seedlings biomass, and did not assist element absorption of plants. Although three strains of EMF can all enhance the tolerance of pine seedlings to salinity stress, they may utilize different mechanisms because of different performance in absorption of elements. The increased P and K accumulation and the balance of ion concentrations might be due to the enhanced tolerance of EMF-inoculated plants to saline conditions.

A Fuzzy-based Methodology for an Aggregative Environmental Risk Assessment of Restored Soil

Environmental risks pertaining to contaminated soils have been well studied,while little attention has been paid to the risks of the soils after remediation. In this study,a concept model developed based on fuzzy set theory was applied to evaluate the uncertainties of three risk indicators,namely,plant growth,groundwater safety and human health,of a restored site that had been previously polluted by heavy metals. The concept model classified the grade and importance of risk factors by an 11-level ranking system and was able to yield a comprehensive risk result rather than multi-risk results for complex risk indicators. Modeling results showed that the risks to the three indicators were effectively reduced after the remediation. Moreover,great sensitivity of the risks was found related to the weight distribution among the three risk indicators. In general,the risks of both polluted and restored soils to the environment were in the order of groundwater safety > plant growth > human health. The model was proved to solve the problems of multi-risk results due to complex risk indicators that previously encountered by other researchers,which made it helpful in decision-making and management of restored soils.

Effect of substance P on gene expression of transforming growth factor β-1 and its receptors in rat's fibroblasts

Objective: To investigate the effect of substance P (SP) on gene expression of transforming growth factor β-1 (TGFβ-1), transforming growth factor receptor-1 (TGFR-1) and transforming growth factor receptor-2 (TGFR-2) in fibroblasts cultured in vitro from rat’s granulation tissues. Methods: The fibroblasts from the granulation tissues in the skeletal muscle of rat’s hind limbs injured by formaldehyde were cultured in vitro. When different concentrations (10 -9-10 -5 mol/L) of SP were added into the culture medium, the changes of gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the cultured fibroblasts were observed with reverse transcription polymerase chain reaction at different intervals (0, 3, 6, 12 and 24 hours after incubation). Results: The gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the fibroblasts cultured from rat’s granulation tissues was up-regulated by SP. The peak level of the mRNA expression was found at 10 -8 mol/L SP and the up-regulation effect was not found at 10 -5 mol/L and 10 -6 mol/L. The peak levels of gene expression of TGFβ-1, TGFR-1 and TGFR-2 in the fibroblasts treated with SP were achieved at 6 and 12 hours, respectively. Conclusions: SP has up-regulation effect on the gene expression of TGFβ-1, TGFR-1 and TGFR-2 in fibroblasts from rat’s granulation tissues in vitro, and the effect is related to different stimulating concentrations of SP. It may be concerned with proliferation and differentiation of fibroblasts and formation of scar tissues during wound healing.