Atlantic salmon Salmo salar were reared at four stocking densities--high density D1 (final density -39 kg/m^3), medium densities DE (-29 kg/m^3) and D3 (~19 kg/m^3), and low density D4 (-12 kg/m^3)- for 40 day...Atlantic salmon Salmo salar were reared at four stocking densities--high density D1 (final density -39 kg/m^3), medium densities DE (-29 kg/m^3) and D3 (~19 kg/m^3), and low density D4 (-12 kg/m^3)- for 40 days to investigate the effect of stocking density on their growth performance, body composition and energy budgets. Stocking density did not significantly affect specific growth rate in terms of weight (SGRw) but did affect specific growth rate in terms of energy (SGRe). Stocking density significantly influenced the ration level (RLw and RLe), feed conversion ratio (FCRw and FCRe) and apparent digestibility rate (ADR). Ration level and FCRw tended to increase with increasing density. Fish at the highest density D~ and lowest density D4 showed lower FCRe and higher ADR than at medium densities. Stocking density significantly affected protein and energy contents of the body but did not affect its moisture, lipid, or ash contents. The expenditure of energy for metabolism in the low-density and high-density groups was lower than that in the medium-density groups. Stocking density affected energy utilization from the feces but had no effect on excretion rate. The greater energy allocation to growth at high density and low density may be attributed to reduced metabolic rate and increased apparent digestibility rate. These findings provide information that will assist selection of suitable stocking densities in the Atlantic-salmon-farming industry.展开更多
In this study, specific growth rate(SGR), ingestion rate(IR), food conversion ratio(FCR), apparent digestion ratio(ADR) and ammonium-nitrogen excretion were determined for sea cucumber(Stichopus monotuberculatus) rear...In this study, specific growth rate(SGR), ingestion rate(IR), food conversion ratio(FCR), apparent digestion ratio(ADR) and ammonium-nitrogen excretion were determined for sea cucumber(Stichopus monotuberculatus) reared in plastic containers(70 L; 4 containers each diet treatment). Sea cucumbers were fed with five diets containing different amounts of farming waste from shrimp(Litopenaeus vannamei)(100%, 75%, 50%, 25% and 0) and a formulated compound(20% sea mud and 80% powdered algae). Sea cucumbers grew faster when they were fed with diet D(25% shrimp waste and 75% formulated compound) than those fed with other diets. Although IR value of sea cucumber fed with diet A(shrimp waste) was higher than those fed with other diets, both the lowest SGR and the highest FCR occurred in this diet group. The highest and the lowest ADR occurred in diet E(formulated compound) and diet A group, respectively, and the same to ammonium-nitrogen excretion. The contents of crude protein, crude lipid and total organic matter(TOM) in feces decreased in comparison with corresponding diets. In the feces from different diet treatments, the contents of crude protein and TOM increased gradually as the contents of crude protein and TOM in diets increased, while crude lipid content decreased gradually as the crude lipid content in diets increased.展开更多
We evaluated the effect of ration level (RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok (initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration level...We evaluated the effect of ration level (RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok (initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation, 2%, 3%, 4% bwd (body weight per day, based on initial mean values), and apparent satiation. Feed consumption, apparent digestibility, and growth were directly measured. Specific growth rates in terms of wet weight, dry weight, protein, and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight (SGRw, %/d) and RL (%) was characterized by a decelerating curve: SGRw=- 1.417+3.1661n(RL+ 1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level, and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy (FERe) and RL was: FERe=- 14.167+23.793RL-3.367(RL)2, and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 kJ BW (kg)-0.80/d, the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE, where IE is feed energy, FE is fecal energy, ZE+UE is excretory energy, HE is heat production, and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd, whereas for energy growth, the suggested rate is 3.53% bwd at this growth stage.展开更多
We conducted a preliminarily study on the effects of dietary guaiacol on growth performance of abalone, Haliotis discus hannai Ino. Seven semi-purified diets were formulated with graded levels of guaiacol (0, 5, 10, ...We conducted a preliminarily study on the effects of dietary guaiacol on growth performance of abalone, Haliotis discus hannai Ino. Seven semi-purified diets were formulated with graded levels of guaiacol (0, 5, 10, 50, 100, 500, and 1 000 mg kgl). Abalone (initial weight: 0.29 ± 0.01 g; initial shell length: 8.55 ± 0.27 mm) were fed with these diets in a re-circulated water system for 152 days. Guaiacol significantly improved the specific growth rate (SGR) (P〈0.05); excessive dietary guaiacol (1 000 mg kg1) led to significantly high mortality (P〈0.05), and lipid content in the soft body increased significantly after dietary guaiacol (P〈0.05). Activities of catalase and phenoloxidase (PO) in the viscera were significantly stimulated by dietary guaiacol (P〈0.05). Broken-line analysis based on SGR indicated that the minimum dietary guaiacol for the optimal growth of juvenile abalone is 15.43 mg ·kg^-1.展开更多
This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel...This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel cake (PKC), wheat offal (WO), corn bran (CB), rice bran (RB) and brewers' dried grain (BDG) were used at 25% in each of the five experimental diets that were formulated in this study. Twenty weaner pigs (12.75 ± 0.6 kg) were used for the growth study while 15 pigs (14.95 ± 0.57 kg) were used for the digestibility study. The design of the experiment was completely randomized. The results of the study showed that the performance of the experimental animals were significantly influenced (P 〈 0.05) by the dietary treatments. The apparent digestibility of the crude protein, crude fiber and ether extract showed significant differences (P 〈 0.05) while there was no significant difference (P 〉 0.05) in the apparent digestibility of their dry matter, ash and nitrogen free extract. The diet that contained WO had the best growth response and apparent nutrient digestibility. Results of economics of production also showed that diet with WO gave the best (P 〈 0.05) value in feed cost/'kg gain and net profit/animal. It can be concluded from this study that although all the animals fed the different fibre feedstuffs performed satisfactorily on all the parameters monitored, but for optimum growth, apparent nutrients digestibility and economics of production. WO should be used as a fibre feedstuff in the diets of weaning/growing pigs in the tropics.展开更多
An 8-week feeding trial was conducted to investigate the effects of dietary soy isoflavones on feeding intake,growth performance,and digestion of juvenile Japanese flounder(Paralichthys olivaceus).Four isonitrogenous(...An 8-week feeding trial was conducted to investigate the effects of dietary soy isoflavones on feeding intake,growth performance,and digestion of juvenile Japanese flounder(Paralichthys olivaceus).Four isonitrogenous(49% crude protein) and isoenergetic(20.1 MJ kg-1) diets were formulated to contain four graded levels of soy isoflavones,namely,0,1,4 and 8 g soy isoflavones in 1 kg of diet.Each diet was randomly fed to triplicate tanks of fish(Initial average weight:2.58 g ± 0.01 g),and each tank was stocked with 35 fish.No significant difference was observed among diets with levels of 0,1 and 4 g kg-1 soy isoflavones in feed intake,weight gain,feed efficiency ratio(FER),proximate composition of fish whole body and apparent digestibility coefficients(ADC) of nutrients and energy(P>0.05).However,high dietary soy isoflavones level(8 g kg-1) significantly depressed weight gain,FER,whole-body crude lipid content of fish and ADC of nutrients(P<0.05).These results indicate that high level of dietary soy isoflavones(above 4 g kg-1) significantly depresses growth responses and FER of Japanese flounder.However,as the content of soy isoflavones in soybean meal is around 1 to 3 g kg-1,the adverse effects might be neglected when soybean products are used as a fish feed ingredient.展开更多
In the interest of decreasing feed costs while maximizing the growth of weanling horses, this study examined the potential difference in the average daily gain (ADG), gain to feed ratio (G/F), and apparent dry mat...In the interest of decreasing feed costs while maximizing the growth of weanling horses, this study examined the potential difference in the average daily gain (ADG), gain to feed ratio (G/F), and apparent dry matter and crude protein digestibility in horses fed with the same diet provided in different feeder types. Sixteen weanling standardbred horses were used for the duration of the 9-week study. The horses were given 2.5% of their body weight in hay and grain daily, 2% hay and 0.5% grain, as fed in two equal feedings at 7:00 am and 7:00 pm. The first treatment used a combined hay and grain feeder with the hay rack positioned over a manger compartment. The second treatment provided hay in an open hay rack and grain in a separate, open-topped feed tub, not positioned under the hay rack. The two groups, with eight horses--four female and four male in each group, were fed in one manner or the other for the length of the study. The weanlings fed in the combined hay and grain feeder had a greater ADG (0.52 kg/d vs. 0.41 kg/d) and G/F (0.06 vs. 0.05) than the horses with the separate hay and grain feeders. Both ADG and G/F means between treatments were statistically different (P 〈 0.05). There was not a significant difference (P 〉 0.05) in the apparent digestibility of dry matter or crude protein between the two groups. Therefore, it was concluded that the combined feeding system resulted in greater growth and apparent G/F most likely due to decreased feed wastage; however, apparent digestibility was not different between feeding systems.展开更多
Objective: The aim of the study was to investigate the human epidermal growth factor receptor 2(HER2) gene amplification and protein expression and interpretation points in the stomach mixed carcinomas. Methods: Immun...Objective: The aim of the study was to investigate the human epidermal growth factor receptor 2(HER2) gene amplification and protein expression and interpretation points in the stomach mixed carcinomas. Methods: Immunohistochemistry(IHC) and fluorescence in situ hybridization(FISH) technique were used to detect HER2 gene amplification and expression of HER2 protein in 442 cases of gastric mixed carcinoma. Results: The expression rate of HER2 protein was 41.2%(182/442): the HER2 protein expression IHC 3+ extensive type in 18 cases, partial type in 21 cases, focal type in 8 cases, accounting for 10.6%(47/442); the HER2 protein expression IHC 2+ extensive type in 23 cases, partial type in 28 cases, focal type in 11 cases, accounting for 14.0%(62/442); the HER2 protein expression IHC 1+ extensive type in 27 cases, partial type in 31 cases, focal type in 15 cases, accounting for 16.5%(73/442). HER2 gene amplification rate of 442 cases was 16.1%(71/442). In 182 cases of HER2 protein positive expression, the HER2 gene cluster amplification rate was 14.8%(27/182), large granular amplification rate 11.0%(20/182), punctate amplification rate 6.0%(11/182) and high polysomy 7.1%(13/182). In 71 cases of HER2 gene amplification, there was 42 cases of HER2 protein expression IHC 3+, 22 cases of HER2 protein expression IHC 2+, and 7 cases of IHC 1+. Conclusion: HER2 detection of gastric mixed carcinoma has great heterogeneity, HER2 protein positive expression is divided into extensive type, partial type and focal type, and HER2 gene positive amplification is divided into cluster amplification, large granular amplification, punctate amplification and high polysomy. These typing of HER2 protein expression and HER2 gene amplification provide reference index to quantify for targeted therapeutic effect of anticancer drugs.展开更多
基金Supported by the National Natural Science Foundation of China(No.31240012)the Earmarked Fund for Modern Agro-industry Technology Research System+2 种基金the Special Foundation for Postdoctoral Innovative Projects of Shandong Province(No.201101009)the National Key Technology R&D Program of China(No.2011BAD13B04)The Key Laboratory of Mariculture(KLM),Ministry of Education,Ocean University of China(OUC)
文摘Atlantic salmon Salmo salar were reared at four stocking densities--high density D1 (final density -39 kg/m^3), medium densities DE (-29 kg/m^3) and D3 (~19 kg/m^3), and low density D4 (-12 kg/m^3)- for 40 days to investigate the effect of stocking density on their growth performance, body composition and energy budgets. Stocking density did not significantly affect specific growth rate in terms of weight (SGRw) but did affect specific growth rate in terms of energy (SGRe). Stocking density significantly influenced the ration level (RLw and RLe), feed conversion ratio (FCRw and FCRe) and apparent digestibility rate (ADR). Ration level and FCRw tended to increase with increasing density. Fish at the highest density D~ and lowest density D4 showed lower FCRe and higher ADR than at medium densities. Stocking density significantly affected protein and energy contents of the body but did not affect its moisture, lipid, or ash contents. The expenditure of energy for metabolism in the low-density and high-density groups was lower than that in the medium-density groups. Stocking density affected energy utilization from the feces but had no effect on excretion rate. The greater energy allocation to growth at high density and low density may be attributed to reduced metabolic rate and increased apparent digestibility rate. These findings provide information that will assist selection of suitable stocking densities in the Atlantic-salmon-farming industry.
基金supported by the Key Project of National Science & Technology Pillar Program in 12th Five-year Plan (2011BAD13B02, 2012BAD18B03)the Science & Technology Promoting Project for Oceanic & Fishery in Guangdong Province (A201100D01, A201101 D02)+3 种基金the Knowledge Innovation Key Project of Chinese Academy of Sciences (KZCX2-EW-Q212)the comprehensive strategic cooperation project of Guangdong Province and Chinese Academy of Sciences (2012B09 1100269)the Cooperation Program of Guangdong Province and Chinese Academy of Sciences (2012B0911 00272)the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (2014KQNCX183)
文摘In this study, specific growth rate(SGR), ingestion rate(IR), food conversion ratio(FCR), apparent digestion ratio(ADR) and ammonium-nitrogen excretion were determined for sea cucumber(Stichopus monotuberculatus) reared in plastic containers(70 L; 4 containers each diet treatment). Sea cucumbers were fed with five diets containing different amounts of farming waste from shrimp(Litopenaeus vannamei)(100%, 75%, 50%, 25% and 0) and a formulated compound(20% sea mud and 80% powdered algae). Sea cucumbers grew faster when they were fed with diet D(25% shrimp waste and 75% formulated compound) than those fed with other diets. Although IR value of sea cucumber fed with diet A(shrimp waste) was higher than those fed with other diets, both the lowest SGR and the highest FCR occurred in this diet group. The highest and the lowest ADR occurred in diet E(formulated compound) and diet A group, respectively, and the same to ammonium-nitrogen excretion. The contents of crude protein, crude lipid and total organic matter(TOM) in feces decreased in comparison with corresponding diets. In the feces from different diet treatments, the contents of crude protein and TOM increased gradually as the contents of crude protein and TOM in diets increased, while crude lipid content decreased gradually as the crude lipid content in diets increased.
基金Supported by the Special Fund for Agro-Scientific Research in the Public Interest of China(No.201003055)the National Key Technology Research and Development Program of China(Nos.2012BAD25B10,2012BAD26B05)the Central-Level Non-Profit Scientific Research Institutes Special Funds of China(No.HSY201412)
文摘We evaluated the effect of ration level (RL) on the growth and energy budget of lenok Brachymystax lenok. Juvenile lenok (initial mean body weight 3.06±0.13 g) were fed for 21 d at five different ration levels: starvation, 2%, 3%, 4% bwd (body weight per day, based on initial mean values), and apparent satiation. Feed consumption, apparent digestibility, and growth were directly measured. Specific growth rates in terms of wet weight, dry weight, protein, and energy increased logarithmically with an increase in ration levels. The relationship between specific growth rate in terms of wet weight (SGRw, %/d) and RL (%) was characterized by a decelerating curve: SGRw=- 1.417+3.1661n(RL+ 1). The apparent digestibility coefficients of energy exhibited a decreasing pattern with increasing ration level, and there was a significant difference among different RLs. Body composition was significantly affected by ration size. The relationship between feed efficiency rate in terms of energy (FERe) and RL was: FERe=- 14.167+23.793RL-3.367(RL)2, and the maximum FERe was observed at a 3.53% ration. The maintenance requirement for energy of juvenile lenok was 105.39 kJ BW (kg)-0.80/d, the utilization efficiency of DE for growth was 0.496. The energy budget equation at satiation was: 100IE=29.03FE+5.78(ZE+UE)+39.56 HE+25.63 RE, where IE is feed energy, FE is fecal energy, ZE+UE is excretory energy, HE is heat production, and RE is recovered energy. Our results suggest that the most suitable feeding rate for juvenile lenok aquaculture for wet weight growth is 2.89% bwd, whereas for energy growth, the suggested rate is 3.53% bwd at this growth stage.
基金Supported by the Program for New Century Excellent Talents in University (NCET) (No.NCET-07-0785)
文摘We conducted a preliminarily study on the effects of dietary guaiacol on growth performance of abalone, Haliotis discus hannai Ino. Seven semi-purified diets were formulated with graded levels of guaiacol (0, 5, 10, 50, 100, 500, and 1 000 mg kgl). Abalone (initial weight: 0.29 ± 0.01 g; initial shell length: 8.55 ± 0.27 mm) were fed with these diets in a re-circulated water system for 152 days. Guaiacol significantly improved the specific growth rate (SGR) (P〈0.05); excessive dietary guaiacol (1 000 mg kg1) led to significantly high mortality (P〈0.05), and lipid content in the soft body increased significantly after dietary guaiacol (P〈0.05). Activities of catalase and phenoloxidase (PO) in the viscera were significantly stimulated by dietary guaiacol (P〈0.05). Broken-line analysis based on SGR indicated that the minimum dietary guaiacol for the optimal growth of juvenile abalone is 15.43 mg ·kg^-1.
文摘This study was conducted to investigate the effect of feeding different fibre feedstuffs on the performance, nutrient utilization and economics of production of weaning/growing pigs. Five fibre feedstuffs--palm kernel cake (PKC), wheat offal (WO), corn bran (CB), rice bran (RB) and brewers' dried grain (BDG) were used at 25% in each of the five experimental diets that were formulated in this study. Twenty weaner pigs (12.75 ± 0.6 kg) were used for the growth study while 15 pigs (14.95 ± 0.57 kg) were used for the digestibility study. The design of the experiment was completely randomized. The results of the study showed that the performance of the experimental animals were significantly influenced (P 〈 0.05) by the dietary treatments. The apparent digestibility of the crude protein, crude fiber and ether extract showed significant differences (P 〈 0.05) while there was no significant difference (P 〉 0.05) in the apparent digestibility of their dry matter, ash and nitrogen free extract. The diet that contained WO had the best growth response and apparent nutrient digestibility. Results of economics of production also showed that diet with WO gave the best (P 〈 0.05) value in feed cost/'kg gain and net profit/animal. It can be concluded from this study that although all the animals fed the different fibre feedstuffs performed satisfactorily on all the parameters monitored, but for optimum growth, apparent nutrients digestibility and economics of production. WO should be used as a fibre feedstuff in the diets of weaning/growing pigs in the tropics.
基金supported by the National Natural Science Foundation of China (No. 30371120)Modern Agro-industry Technology Research System (No. nycytx50-G07)
文摘An 8-week feeding trial was conducted to investigate the effects of dietary soy isoflavones on feeding intake,growth performance,and digestion of juvenile Japanese flounder(Paralichthys olivaceus).Four isonitrogenous(49% crude protein) and isoenergetic(20.1 MJ kg-1) diets were formulated to contain four graded levels of soy isoflavones,namely,0,1,4 and 8 g soy isoflavones in 1 kg of diet.Each diet was randomly fed to triplicate tanks of fish(Initial average weight:2.58 g ± 0.01 g),and each tank was stocked with 35 fish.No significant difference was observed among diets with levels of 0,1 and 4 g kg-1 soy isoflavones in feed intake,weight gain,feed efficiency ratio(FER),proximate composition of fish whole body and apparent digestibility coefficients(ADC) of nutrients and energy(P>0.05).However,high dietary soy isoflavones level(8 g kg-1) significantly depressed weight gain,FER,whole-body crude lipid content of fish and ADC of nutrients(P<0.05).These results indicate that high level of dietary soy isoflavones(above 4 g kg-1) significantly depresses growth responses and FER of Japanese flounder.However,as the content of soy isoflavones in soybean meal is around 1 to 3 g kg-1,the adverse effects might be neglected when soybean products are used as a fish feed ingredient.
文摘In the interest of decreasing feed costs while maximizing the growth of weanling horses, this study examined the potential difference in the average daily gain (ADG), gain to feed ratio (G/F), and apparent dry matter and crude protein digestibility in horses fed with the same diet provided in different feeder types. Sixteen weanling standardbred horses were used for the duration of the 9-week study. The horses were given 2.5% of their body weight in hay and grain daily, 2% hay and 0.5% grain, as fed in two equal feedings at 7:00 am and 7:00 pm. The first treatment used a combined hay and grain feeder with the hay rack positioned over a manger compartment. The second treatment provided hay in an open hay rack and grain in a separate, open-topped feed tub, not positioned under the hay rack. The two groups, with eight horses--four female and four male in each group, were fed in one manner or the other for the length of the study. The weanlings fed in the combined hay and grain feeder had a greater ADG (0.52 kg/d vs. 0.41 kg/d) and G/F (0.06 vs. 0.05) than the horses with the separate hay and grain feeders. Both ADG and G/F means between treatments were statistically different (P 〈 0.05). There was not a significant difference (P 〉 0.05) in the apparent digestibility of dry matter or crude protein between the two groups. Therefore, it was concluded that the combined feeding system resulted in greater growth and apparent G/F most likely due to decreased feed wastage; however, apparent digestibility was not different between feeding systems.
基金Supported by a grant from the Henan Provincial Key Scientific and Technological Project(No.132102310008)
文摘Objective: The aim of the study was to investigate the human epidermal growth factor receptor 2(HER2) gene amplification and protein expression and interpretation points in the stomach mixed carcinomas. Methods: Immunohistochemistry(IHC) and fluorescence in situ hybridization(FISH) technique were used to detect HER2 gene amplification and expression of HER2 protein in 442 cases of gastric mixed carcinoma. Results: The expression rate of HER2 protein was 41.2%(182/442): the HER2 protein expression IHC 3+ extensive type in 18 cases, partial type in 21 cases, focal type in 8 cases, accounting for 10.6%(47/442); the HER2 protein expression IHC 2+ extensive type in 23 cases, partial type in 28 cases, focal type in 11 cases, accounting for 14.0%(62/442); the HER2 protein expression IHC 1+ extensive type in 27 cases, partial type in 31 cases, focal type in 15 cases, accounting for 16.5%(73/442). HER2 gene amplification rate of 442 cases was 16.1%(71/442). In 182 cases of HER2 protein positive expression, the HER2 gene cluster amplification rate was 14.8%(27/182), large granular amplification rate 11.0%(20/182), punctate amplification rate 6.0%(11/182) and high polysomy 7.1%(13/182). In 71 cases of HER2 gene amplification, there was 42 cases of HER2 protein expression IHC 3+, 22 cases of HER2 protein expression IHC 2+, and 7 cases of IHC 1+. Conclusion: HER2 detection of gastric mixed carcinoma has great heterogeneity, HER2 protein positive expression is divided into extensive type, partial type and focal type, and HER2 gene positive amplification is divided into cluster amplification, large granular amplification, punctate amplification and high polysomy. These typing of HER2 protein expression and HER2 gene amplification provide reference index to quantify for targeted therapeutic effect of anticancer drugs.