研究弗赖森草螺菌RE3-3菌株的培养条件对其生长素(IAA)生物合成的影响,并观察菌株合成IAA对芦苇及香蒲幼苗的促生影响。在单因素筛选和菌液初始浓度确定的基础上,对菌株RE3-3合成IAA的培养条件进行优化;以IAA标准物质为对照,对菌株合成...研究弗赖森草螺菌RE3-3菌株的培养条件对其生长素(IAA)生物合成的影响,并观察菌株合成IAA对芦苇及香蒲幼苗的促生影响。在单因素筛选和菌液初始浓度确定的基础上,对菌株RE3-3合成IAA的培养条件进行优化;以IAA标准物质为对照,对菌株合成IAA促进芦苇及香蒲苗的生长能力进行分析。通过高效液相色谱与质谱分析鉴定其产物为IAA;培养基中含0.07%L-色氨酸及1%葡萄糖,在p H 7.0、温度30℃、培养时间48 h的最佳培养条件下,菌株RE3-3可产生最大量的IAA(74 mg/L);菌株合成IAA作用芦苇及香蒲苗时,其促生效果最显著,植株根长、茎长明显提高。通过改变菌株RE3-3的培养条件,可提高其合成IAA的能力,且菌株RE3-3合成IAA具有显著的促生效果。展开更多
An HPLC method was used to analyze the contents and variation of IAA,GA3,ABA.and ZT at five stages around the tea shoot germinating in spring.The contents of GAa and ABA had a top value during the winter and decreased...An HPLC method was used to analyze the contents and variation of IAA,GA3,ABA.and ZT at five stages around the tea shoot germinating in spring.The contents of GAa and ABA had a top value during the winter and decreased with the growth of tea shoots,while the contents of IAA and ZT had a low value during the winter and increased quickly at the beginning of shoot growth,but soon afterwards increased slowly or decreased a little.The ratio of hormones was closely related to the growth of tea plant.The study indicated that the ratios of GA3 to ABA and IAA to ABA were at low values during the winter and went up with the shoot genninating.When the activity of roots was weak,the ratio of ZT to IAA had a top value,but went down gradually with luxuriant activity of roots.The ratio of GA3 to ZT had a certain relativity with the shoot genninating,which was at a top value during the winter but went down suddenly at the beginning of shoot genninating.展开更多
文摘研究弗赖森草螺菌RE3-3菌株的培养条件对其生长素(IAA)生物合成的影响,并观察菌株合成IAA对芦苇及香蒲幼苗的促生影响。在单因素筛选和菌液初始浓度确定的基础上,对菌株RE3-3合成IAA的培养条件进行优化;以IAA标准物质为对照,对菌株合成IAA促进芦苇及香蒲苗的生长能力进行分析。通过高效液相色谱与质谱分析鉴定其产物为IAA;培养基中含0.07%L-色氨酸及1%葡萄糖,在p H 7.0、温度30℃、培养时间48 h的最佳培养条件下,菌株RE3-3可产生最大量的IAA(74 mg/L);菌株合成IAA作用芦苇及香蒲苗时,其促生效果最显著,植株根长、茎长明显提高。通过改变菌株RE3-3的培养条件,可提高其合成IAA的能力,且菌株RE3-3合成IAA具有显著的促生效果。
文摘An HPLC method was used to analyze the contents and variation of IAA,GA3,ABA.and ZT at five stages around the tea shoot germinating in spring.The contents of GAa and ABA had a top value during the winter and decreased with the growth of tea shoots,while the contents of IAA and ZT had a low value during the winter and increased quickly at the beginning of shoot growth,but soon afterwards increased slowly or decreased a little.The ratio of hormones was closely related to the growth of tea plant.The study indicated that the ratios of GA3 to ABA and IAA to ABA were at low values during the winter and went up with the shoot genninating.When the activity of roots was weak,the ratio of ZT to IAA had a top value,but went down gradually with luxuriant activity of roots.The ratio of GA3 to ZT had a certain relativity with the shoot genninating,which was at a top value during the winter but went down suddenly at the beginning of shoot genninating.
