[Objective] The aim was to study rooting environment of strawberry ＂Bechi- ka＂ seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence f...[Objective] The aim was to study rooting environment of strawberry ＂Bechi- ka＂ seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence factom of rooting and the effects of different transplanting substrata on survival rate was investigated. [Result] The results showed that： 1/2MS was the best concentration for rooting and the rooting rate could reach 100%. IBA was better than IAA for rooting on growth regu- lator levdls, and it had the best effect on rooting with 0.3 mg/L, which produced the thickest and most-fibrous roots. Activated carbon had positive influences on the growth of roots, and the suitable concentration of activated carbon was 0.5 mg/l_ for rooting. The study found that survival rate of tissue culture seedlings was obviously higher in the combination of perlite and vermiculite than that in the combination of humus and sand, and the survival rate could reach 98.1% （perlite：vermiculite=l：l）. Seedlings in various transplanting substrata could normally bloom, fruit and form stolons. [Conclusion] The study provided technical support for achieving large-scale production of virus-free strawberry and quickly cultivating good seedlings.展开更多
Salt-affected soils are agricultural and environmental problems on a global scale. Plants suffer from saline stresses in these soils and show nitrogen （N） deficiency symptoms. However, halophytes grow soundly under ...Salt-affected soils are agricultural and environmental problems on a global scale. Plants suffer from saline stresses in these soils and show nitrogen （N） deficiency symptoms. However, halophytes grow soundly under saline conditions. In order to clarify the N nutrition of the halophyte Salicornia bigelovii, it was grown at several N levels （1, 2, 3, and 4 mmol L-1）, supplied in the form of NO3 or ammonium （NH4＋）, under high NaCl conditions （200 mmol L-l）. NH4^＋- fed plants showed better growth than NO3-fed plants at 1-3 mmol L-1N, and plants in both treatments showed the same growth at 4 mmol L-1 N. Nitrogen contents in NO3-fed plants increased with the N concentrations in solution; competitive inhibition of NO3- absorption by Cl- was observed under lower N conditions. In addition, shoot dry weight was significantly correlated only with shoot N content. Therefore, growth of NO3-fed plants was regulated by N absorption. In contrast, N contents of shoots in NH4＋-fed plants did not change with N concentration. Shoot Na content decreased with increasing N concentration, while K content increased. Dry weight was highly correlated only with K content in NH4＋-fed plants. These observations indicated that growth of NH4＋-fed plants was mainly regulated by K absorption.展开更多
Objective： The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma...Objective： The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma cell lines. Methods： The medullary thyroid carcinoma cell line was cultured in vitro, with parathyroid hormone and parathyroid hormone receptor monoclonal antibody treatment intervention, the growth of the cells was observed under an inverted contrast micro scope, the MTT assay was used to detect the cell growth inhibition rate. Results： Under the inverted contrast microscope, the cells changed significantly, the parathyroid hormone and parathyroid hormone receptor monoclonal antibodies can effectively inhibit the proliferation of medullary thyroid cancer cells in a time and dose dependent. When parathyroid hormone concentra tion reached a concentration of 2.0 IJmol/L, the parathyroid hormone receptor monoclonal antibody reached a concentration of 1.0 μmol/L, the cell growth was most significantly inhibited （P 〈 0.05）. Conclusion： Parathyroid hormone and parathyroid hormone receptor monoclonal antibody were able to inhibit the proliferation of medullary thyroid carcinoma cells and signifi cantly reduce the proliferation index.展开更多
The object of this study was to adapt in vitro system for morphogenesis and regeneration of microshoots of common milkweed （Asclepias syriaca L.） applying different concentrations of hydrogen ion （H＋） and cytokin...The object of this study was to adapt in vitro system for morphogenesis and regeneration of microshoots of common milkweed （Asclepias syriaca L.） applying different concentrations of hydrogen ion （H＋） and cytokinin 6-benzylaminopurine （BAP）. The influence of BAP and hydrogen ion （H＋） on the level of primary （chlorophyll a, chlorophyll b and carotenoids） and secondary （flavonoids and hydrolyzable and condensed tannins） metabolites in in vitro grown Asclepias syriaca L, were evaluated. Six different concentrations of BAP （0, 1, 2, 3, 4 and 5 ~tmol/L） and three different concentrations of hydrogen ion （pH 4.5, 5.0 and 5.5） were applied to the woody plant medium （WPM） medium used for microshoots propagation. The most effective morphogenesis of Asclepias syriaca L. was observed in culture medium supplemented with 2 p, mol/L BAP. However, synthesis of primary and secondary metabolites was the most intensive when cytokinin concentration reached the value of 3 gmol/L BAP. It was determined that the activity of hydrogen ion （H＋）, measured as the pH of culture medium, had a significant effect on secondary metabolites in the shoots in vitro.展开更多
Pinus tabulaeformis seedlings were grown under a factorial design to measure biomass accumulation and P, Na and K concentrations of plants, with 3 different ectomycorrhizal fungus (EMF) strains (Boletus edulis, Xeroco...Pinus tabulaeformis seedlings were grown under a factorial design to measure biomass accumulation and P, Na and K concentrations of plants, with 3 different ectomycorrhizal fungus (EMF) strains (Boletus edulis, Xerocomus chrysenteron and Gomphidius viscidus) and two P treatments (with or without extra P). Growth and mineral nutrition of pine seedlings were stimulated by three EMF under salinity stress. Na concentrations were changed and plant K and P concentrations were increased significantly by EMF. Adding extra P made no difference in accumulation of seedlings biomass, and did not assist element absorption of plants. Although three strains of EMF can all enhance the tolerance of pine seedlings to salinity stress, they may utilize different mechanisms because of different performance in absorption of elements. The increased P and K accumulation and the balance of ion concentrations might be due to the enhanced tolerance of EMF-inoculated plants to saline conditions.展开更多
文摘[Objective] The aim was to study rooting environment of strawberry ＂Bechi- ka＂ seedlings, and the somaclone domesticated transplanting was carded on. [Method] With the stem tip of stolon as material, the influence factom of rooting and the effects of different transplanting substrata on survival rate was investigated. [Result] The results showed that： 1/2MS was the best concentration for rooting and the rooting rate could reach 100%. IBA was better than IAA for rooting on growth regu- lator levdls, and it had the best effect on rooting with 0.3 mg/L, which produced the thickest and most-fibrous roots. Activated carbon had positive influences on the growth of roots, and the suitable concentration of activated carbon was 0.5 mg/l_ for rooting. The study found that survival rate of tissue culture seedlings was obviously higher in the combination of perlite and vermiculite than that in the combination of humus and sand, and the survival rate could reach 98.1% （perlite：vermiculite=l：l）. Seedlings in various transplanting substrata could normally bloom, fruit and form stolons. [Conclusion] The study provided technical support for achieving large-scale production of virus-free strawberry and quickly cultivating good seedlings.
基金Supported by the "Global Center of Excellence for Dryland Science",a project of the Ministry of Education,Science,Culture,Sports and Technology of Japan
文摘Salt-affected soils are agricultural and environmental problems on a global scale. Plants suffer from saline stresses in these soils and show nitrogen （N） deficiency symptoms. However, halophytes grow soundly under saline conditions. In order to clarify the N nutrition of the halophyte Salicornia bigelovii, it was grown at several N levels （1, 2, 3, and 4 mmol L-1）, supplied in the form of NO3 or ammonium （NH4＋）, under high NaCl conditions （200 mmol L-l）. NH4^＋- fed plants showed better growth than NO3-fed plants at 1-3 mmol L-1N, and plants in both treatments showed the same growth at 4 mmol L-1 N. Nitrogen contents in NO3-fed plants increased with the N concentrations in solution; competitive inhibition of NO3- absorption by Cl- was observed under lower N conditions. In addition, shoot dry weight was significantly correlated only with shoot N content. Therefore, growth of NO3-fed plants was regulated by N absorption. In contrast, N contents of shoots in NH4＋-fed plants did not change with N concentration. Shoot Na content decreased with increasing N concentration, while K content increased. Dry weight was highly correlated only with K content in NH4＋-fed plants. These observations indicated that growth of NH4＋-fed plants was mainly regulated by K absorption.
基金Supported by a grant from the Science and Technology Plan Projects of Lanzhou(No.2013-3-38)
文摘Objective： The purpose of the study was to investigate the effects of parathyroid hormone and parathyroid hormone receptor monoclonal antibody on in vitro growth and proliferation of human medullary thyroid carcinoma cell lines. Methods： The medullary thyroid carcinoma cell line was cultured in vitro, with parathyroid hormone and parathyroid hormone receptor monoclonal antibody treatment intervention, the growth of the cells was observed under an inverted contrast micro scope, the MTT assay was used to detect the cell growth inhibition rate. Results： Under the inverted contrast microscope, the cells changed significantly, the parathyroid hormone and parathyroid hormone receptor monoclonal antibodies can effectively inhibit the proliferation of medullary thyroid cancer cells in a time and dose dependent. When parathyroid hormone concentra tion reached a concentration of 2.0 IJmol/L, the parathyroid hormone receptor monoclonal antibody reached a concentration of 1.0 μmol/L, the cell growth was most significantly inhibited （P 〈 0.05）. Conclusion： Parathyroid hormone and parathyroid hormone receptor monoclonal antibody were able to inhibit the proliferation of medullary thyroid carcinoma cells and signifi cantly reduce the proliferation index.
文摘The object of this study was to adapt in vitro system for morphogenesis and regeneration of microshoots of common milkweed （Asclepias syriaca L.） applying different concentrations of hydrogen ion （H＋） and cytokinin 6-benzylaminopurine （BAP）. The influence of BAP and hydrogen ion （H＋） on the level of primary （chlorophyll a, chlorophyll b and carotenoids） and secondary （flavonoids and hydrolyzable and condensed tannins） metabolites in in vitro grown Asclepias syriaca L, were evaluated. Six different concentrations of BAP （0, 1, 2, 3, 4 and 5 ~tmol/L） and three different concentrations of hydrogen ion （pH 4.5, 5.0 and 5.5） were applied to the woody plant medium （WPM） medium used for microshoots propagation. The most effective morphogenesis of Asclepias syriaca L. was observed in culture medium supplemented with 2 p, mol/L BAP. However, synthesis of primary and secondary metabolites was the most intensive when cytokinin concentration reached the value of 3 gmol/L BAP. It was determined that the activity of hydrogen ion （H＋）, measured as the pH of culture medium, had a significant effect on secondary metabolites in the shoots in vitro.
基金Supported by the National Natural Science Foundation of China (No.4002101)
文摘Pinus tabulaeformis seedlings were grown under a factorial design to measure biomass accumulation and P, Na and K concentrations of plants, with 3 different ectomycorrhizal fungus (EMF) strains (Boletus edulis, Xerocomus chrysenteron and Gomphidius viscidus) and two P treatments (with or without extra P). Growth and mineral nutrition of pine seedlings were stimulated by three EMF under salinity stress. Na concentrations were changed and plant K and P concentrations were increased significantly by EMF. Adding extra P made no difference in accumulation of seedlings biomass, and did not assist element absorption of plants. Although three strains of EMF can all enhance the tolerance of pine seedlings to salinity stress, they may utilize different mechanisms because of different performance in absorption of elements. The increased P and K accumulation and the balance of ion concentrations might be due to the enhanced tolerance of EMF-inoculated plants to saline conditions.
