By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of...By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of crucian carp by means of microinjection, the transgenic crucian carp was obtained. The result of PCR detection showed that the growth hormone gene of the exogenous carp was integrated into the genome of crucian carp, and the transgenic crucian carp displayed enhancement of its growth.展开更多
Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone fro...Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.展开更多
To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in eart...To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in earthen ponds maintained according to four management regimes： （1） live zooplankton fed to carp larvae （LF）; （2） direct fertilization with poultry manure （PM）; （3） direct fertilization with cowdung （CD）; and （4） a control treatment （C）. There were three replicates for each treatment. The growth of heterotrophic bacteria and pathogenic microorganisms like Aeromonas sp. and Pseudomonas sp. were also examined in response to pond management. Values of dissolved oxygen were significantly higher （P〈0.05） in the water of LF ponds, compared to other treatments, while the PM and CD treatments recorded were significantly higher （P〈0.05） values of PO4 - P, NH4 - N, NO3 - N, NO2 - N, specific conductivity, alkalinity, and BOD, compared to the LF and C treatments. The percentages of organic carbon and total nitrogen in the bottom sediments were higher in the PM and CD treatments compared to LF （P〈0.05）. Average counts of heterotrophic bacteria in the water of PM and CD ponds were significantly higher than other treatments （P〈0.05）. The development of Aeromonas sp. and Pseudomonas sp. were significantly higher （P〈0.05） in the PM and CD treatments. Weight gain of koi carp stocked in LF was significantly higher （P〈0.05） than that of fish in the other treatments. There was a significant difference in the survival rate of koi carp among the treatments ranging from 67.21% in C to 90.11% in LF. The results suggest that raising koi carp larvae in ponds and feeding them exogenously with zooplankton would support high rates of survival and production through maintenance of better water quality and greater abundance of zooplankton in the system. Significantly lower abundance of Aeromonas sp. and Pseudomonas sp. in the LF treatment considerably lowered any possibility of occurrence of bacterial disease.展开更多
文摘By inserting the CMV with HSV TK promoter into the plasmid pBlue-cGH which contained cGH gene, a chimeric expression vector was constructed for transgenic fish. After the vector was transferred into fertilized eggs of crucian carp by means of microinjection, the transgenic crucian carp was obtained. The result of PCR detection showed that the growth hormone gene of the exogenous carp was integrated into the genome of crucian carp, and the transgenic crucian carp displayed enhancement of its growth.
文摘Recombinant eel GH and yeast containing chinook salmon growth hormone (reGH and rcsGH) were incorporated into gelatin and sodium alginate (reGH GS and rcsGH GS) or polymer matrix (reGH HP55) to protect the hormone from proteolytic cleavage in the stomach. The diets containing reGH GS, rcsGH GS, reGH HP55 and free reGH or uncoated rcsGH were administered to red sea bream. Feeding of reGH GS, reGH HP55 and rcsGH GS diets resulted in significant increases in body weight and fork length over those of controls. These results strongly suggest that gelatin and sodium alginate as well as polymer matrix protected the hormone from proteolytic enzymes in the gastrointestinal tract to allow the bioactive hormone to enter the circulation and eventually stimulate fish growth.
文摘To test the effectiveness of introducing live zooplankton against direct manuring in ornamental fish ponds upon their survival and production, larvae of koi carp, Cyprinus carpio L., were cultured for 11 weeks in earthen ponds maintained according to four management regimes： （1） live zooplankton fed to carp larvae （LF）; （2） direct fertilization with poultry manure （PM）; （3） direct fertilization with cowdung （CD）; and （4） a control treatment （C）. There were three replicates for each treatment. The growth of heterotrophic bacteria and pathogenic microorganisms like Aeromonas sp. and Pseudomonas sp. were also examined in response to pond management. Values of dissolved oxygen were significantly higher （P〈0.05） in the water of LF ponds, compared to other treatments, while the PM and CD treatments recorded were significantly higher （P〈0.05） values of PO4 - P, NH4 - N, NO3 - N, NO2 - N, specific conductivity, alkalinity, and BOD, compared to the LF and C treatments. The percentages of organic carbon and total nitrogen in the bottom sediments were higher in the PM and CD treatments compared to LF （P〈0.05）. Average counts of heterotrophic bacteria in the water of PM and CD ponds were significantly higher than other treatments （P〈0.05）. The development of Aeromonas sp. and Pseudomonas sp. were significantly higher （P〈0.05） in the PM and CD treatments. Weight gain of koi carp stocked in LF was significantly higher （P〈0.05） than that of fish in the other treatments. There was a significant difference in the survival rate of koi carp among the treatments ranging from 67.21% in C to 90.11% in LF. The results suggest that raising koi carp larvae in ponds and feeding them exogenously with zooplankton would support high rates of survival and production through maintenance of better water quality and greater abundance of zooplankton in the system. Significantly lower abundance of Aeromonas sp. and Pseudomonas sp. in the LF treatment considerably lowered any possibility of occurrence of bacterial disease.
