A rapid, simple and accurate method using an immunoaffinity column (IAC) and capillary electrophoresis (CE) for the analysis of the major alkaloids in opium is developed. The IAC was synthesized by coupling specific m...A rapid, simple and accurate method using an immunoaffinity column (IAC) and capillary electrophoresis (CE) for the analysis of the major alkaloids in opium is developed. The IAC was synthesized by coupling specific morphine polyclonal antibodies to CNBr-actived Sepharose 4B. The IAC showed high selectivity and obvious enrichment to morphine, codeine, dionin and thebaine. The extraction solution was analyzed by CE with β-cyclodextrin as an additive. Recoveries of the four alkaloids from PBS were between 93%-105% with RSD value less than 5.0%. The result showed that this method was practical for the determination of morphine analogs in opium.展开更多
文摘A rapid, simple and accurate method using an immunoaffinity column (IAC) and capillary electrophoresis (CE) for the analysis of the major alkaloids in opium is developed. The IAC was synthesized by coupling specific morphine polyclonal antibodies to CNBr-actived Sepharose 4B. The IAC showed high selectivity and obvious enrichment to morphine, codeine, dionin and thebaine. The extraction solution was analyzed by CE with β-cyclodextrin as an additive. Recoveries of the four alkaloids from PBS were between 93%-105% with RSD value less than 5.0%. The result showed that this method was practical for the determination of morphine analogs in opium.
