Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as...Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas. Key words glioma - Th1/Th2 - gene expression - RT-PCR This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).展开更多
In 2012-2015, under the conditions of different natural wind speeds, the single-rotor agricultural unmanned aerial vehicle was used for the supplementary pollination during seed production of 10 hybrid combinations wi...In 2012-2015, under the conditions of different natural wind speeds, the single-rotor agricultural unmanned aerial vehicle was used for the supplementary pollination during seed production of 10 hybrid combinations with big parental row ratios in the hybrid rice seed production bases of Hunan, Hainan and Guangdong Province, and the pollination effects were studied through the investigation of pollen density in the field, outcrossing seeding rate of female parent and seed production yield. The results showed that under the parental row ratio of 6：（40-60）, the seed setting rate and yields of the supplementary pollination by single-rotor agricultural UAV could reach and even higher than those of artificial pollination, indicating the single-rotor agricultural UAV could be used in supplementary pollination for hybdd rice seed production, which could promote the whole-process mechanization of seed production.展开更多
Electroencephalogram (EEG) is an efficient tool in exploring human brains. It plays a very important role in diagnosis of disorders related to epilepsy and development of new interaction techniques between machines an...Electroencephalogram (EEG) is an efficient tool in exploring human brains. It plays a very important role in diagnosis of disorders related to epilepsy and development of new interaction techniques between machines and human beings,namely,brain-computer interface (BCI). The purpose of this review is to illustrate the recent researches in EEG processing and EEG-based BCI. First,we outline several methods in removing artifacts from EEGs,and classical algorithms for fatigue detection are discussed. Then,two BCI paradigms including motor imagery and steady-state motion visual evoked potentials (SSMVEP) produced by oscillating Newton's rings are introduced. Finally,BCI systems including wheelchair controlling and electronic car navigation are elaborated. As a new technique to control equipments,BCI has promising potential in rehabilitation of disorders in central nervous system,such as stroke and spinal cord injury,treatment of attention deficit hyperactivity disorder (ADHD) in children and development of novel games such as brain-controlled auto racings.展开更多
Based on the theory that the management is about dynamic ＂relations＂, this paper analyzes the relationship between the construction industry and the ＂peasant laborer＂ in different stages, different contents and di...Based on the theory that the management is about dynamic ＂relations＂, this paper analyzes the relationship between the construction industry and the ＂peasant laborer＂ in different stages, different contents and different aspects. It establishes the three-dimensional model for these relations and points out that a good relationship between the construction enterprise and the construction employees in this crucial time of construction industry is a very crucial link of the achievements, as well as the well-being of the construction enterprises.展开更多
To investigate the inhibitory effect of mycophenolate mofetil on human hepatocellular carcinoma cell line HepG-2. Methods： HepG-2 cells were cultured in the presence of the different concentrations of mycophenolate m...To investigate the inhibitory effect of mycophenolate mofetil on human hepatocellular carcinoma cell line HepG-2. Methods： HepG-2 cells were cultured in the presence of the different concentrations of mycophenolate mofetil in vitro. MTT assay was used to analyze the inhibition of cell viability conferred by mycophenolate mofetil. Cell apoptosis was observed using Hoechst33258 staining, and the percentage of HepG-2 cells at different cell cycles was determined through flow cytometry. The ability of cell adhesion was evaluated by in vitro adhesion assay. Gene expressions of factors （ICAM-1 and VCAM-1） were detected by RT-PCR. Results： Mycophenolate mofetil significantly inhibited the growth of HepG-2 cells by inducing the apoptosis of cells and this drug also inhibited the adhesion of HepG-2 cells in a dose-dependent manner. Marked morphological changes characterized in cell apoptosis were demonstrated through Hoechst33258 staining. In addition, mycophenolate mofetil decreased the proportion of S phase cells and increased that of G0/G1 phase cells. [^3H]-Thymidine uptake assay indicated that the application of mycophenolate mofetil at different concentrations significantly inhibited the cell proliferation. RT-PCR identified the expression levels of ICAM-1 and VCAM-1 genes in liver cancer cells after cultured for 72 h with different concentrations of drug. An inverse relationship was found between the expressions of ICAM-1 and VCAM-1 and drug concentrations. Conclusion： Mycophenolate mofetil has remarkable inhibitory effect on hepatocarcinoma HepG-2 cells.展开更多
Upon flaws of current blockchain platforms of heavyweight, large capacity of ledger, and time-consuming of synchronization of data, in this paper, we proposed a new paradigm of master-slave blockchain scheme(MSB) for ...Upon flaws of current blockchain platforms of heavyweight, large capacity of ledger, and time-consuming of synchronization of data, in this paper, we proposed a new paradigm of master-slave blockchain scheme(MSB) for pervasive computing that suitable for general PC, mobile device such as smart phones or PADs to participants in the working of mining and verification, in which we separated traditional blockchain model in 2 layer defined as master node layer and a series of slavery agents layer, then we proposed 2 approaches for partially computing model(PCM) and non-computing of model(NCM) in the MSB blockchain, Finally large amounts of simulations manifest the proposed master-slave blockchain scheme is feasible, extendible and suitable for pervasive computing especially in the 5 G generation environment, and can apply in the DRM-related applications.展开更多
基金This project was supported by a grant from National Natural Sciences foundation of China(No.30271335).
文摘Objective: To explore the expression of Th1/Th2 cytokines gene in human gliomas and its role in the genesis and development of human gliomas.Methods: Using IL-2 and IFNγ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the biological activity of cytokines in the supernatant of glioma cell lines was assayed by ELISA method, and the gene expression of Th1/Th2 cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR.Results: There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissues.Conclusion: It suggested that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosupressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in the genesis and development of human gliomas. Key words glioma - Th1/Th2 - gene expression - RT-PCR This project was supported by a grant from National Natural Sciences foundation of China (No. 30271335).
基金Supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China(2014BAD06B07)the Rice Industry Technology System of Hunan Province~~
文摘In 2012-2015, under the conditions of different natural wind speeds, the single-rotor agricultural unmanned aerial vehicle was used for the supplementary pollination during seed production of 10 hybrid combinations with big parental row ratios in the hybrid rice seed production bases of Hunan, Hainan and Guangdong Province, and the pollination effects were studied through the investigation of pollen density in the field, outcrossing seeding rate of female parent and seed production yield. The results showed that under the parental row ratio of 6：（40-60）, the seed setting rate and yields of the supplementary pollination by single-rotor agricultural UAV could reach and even higher than those of artificial pollination, indicating the single-rotor agricultural UAV could be used in supplementary pollination for hybdd rice seed production, which could promote the whole-process mechanization of seed production.
基金National Natural Science Foundation of China(No.51005176)Research Fund for the Doctoral Program of Higher Education of China(No.20100201120003)
文摘Electroencephalogram (EEG) is an efficient tool in exploring human brains. It plays a very important role in diagnosis of disorders related to epilepsy and development of new interaction techniques between machines and human beings,namely,brain-computer interface (BCI). The purpose of this review is to illustrate the recent researches in EEG processing and EEG-based BCI. First,we outline several methods in removing artifacts from EEGs,and classical algorithms for fatigue detection are discussed. Then,two BCI paradigms including motor imagery and steady-state motion visual evoked potentials (SSMVEP) produced by oscillating Newton's rings are introduced. Finally,BCI systems including wheelchair controlling and electronic car navigation are elaborated. As a new technique to control equipments,BCI has promising potential in rehabilitation of disorders in central nervous system,such as stroke and spinal cord injury,treatment of attention deficit hyperactivity disorder (ADHD) in children and development of novel games such as brain-controlled auto racings.
文摘Based on the theory that the management is about dynamic ＂relations＂, this paper analyzes the relationship between the construction industry and the ＂peasant laborer＂ in different stages, different contents and different aspects. It establishes the three-dimensional model for these relations and points out that a good relationship between the construction enterprise and the construction employees in this crucial time of construction industry is a very crucial link of the achievements, as well as the well-being of the construction enterprises.
文摘To investigate the inhibitory effect of mycophenolate mofetil on human hepatocellular carcinoma cell line HepG-2. Methods： HepG-2 cells were cultured in the presence of the different concentrations of mycophenolate mofetil in vitro. MTT assay was used to analyze the inhibition of cell viability conferred by mycophenolate mofetil. Cell apoptosis was observed using Hoechst33258 staining, and the percentage of HepG-2 cells at different cell cycles was determined through flow cytometry. The ability of cell adhesion was evaluated by in vitro adhesion assay. Gene expressions of factors （ICAM-1 and VCAM-1） were detected by RT-PCR. Results： Mycophenolate mofetil significantly inhibited the growth of HepG-2 cells by inducing the apoptosis of cells and this drug also inhibited the adhesion of HepG-2 cells in a dose-dependent manner. Marked morphological changes characterized in cell apoptosis were demonstrated through Hoechst33258 staining. In addition, mycophenolate mofetil decreased the proportion of S phase cells and increased that of G0/G1 phase cells. [^3H]-Thymidine uptake assay indicated that the application of mycophenolate mofetil at different concentrations significantly inhibited the cell proliferation. RT-PCR identified the expression levels of ICAM-1 and VCAM-1 genes in liver cancer cells after cultured for 72 h with different concentrations of drug. An inverse relationship was found between the expressions of ICAM-1 and VCAM-1 and drug concentrations. Conclusion： Mycophenolate mofetil has remarkable inhibitory effect on hepatocarcinoma HepG-2 cells.
基金supported by the National Natural Science Foundation of China under Grant 61272519the Research Funds of Blockchain Joint Lab between BUPT and BCTthe joint Blockchain and Security Lab between BUPT and CAPSTONE
文摘Upon flaws of current blockchain platforms of heavyweight, large capacity of ledger, and time-consuming of synchronization of data, in this paper, we proposed a new paradigm of master-slave blockchain scheme(MSB) for pervasive computing that suitable for general PC, mobile device such as smart phones or PADs to participants in the working of mining and verification, in which we separated traditional blockchain model in 2 layer defined as master node layer and a series of slavery agents layer, then we proposed 2 approaches for partially computing model(PCM) and non-computing of model(NCM) in the MSB blockchain, Finally large amounts of simulations manifest the proposed master-slave blockchain scheme is feasible, extendible and suitable for pervasive computing especially in the 5 G generation environment, and can apply in the DRM-related applications.
