[ Objective] The study was to investigate the volatile components of secondary metabolites from M. alpine producing arachidonic acid and explore the changes in its metabolic pathway. [ Method] The air above M. alpine ...[ Objective] The study was to investigate the volatile components of secondary metabolites from M. alpine producing arachidonic acid and explore the changes in its metabolic pathway. [ Method] The air above M. alpine broth was extracted by solid-phase microextraction(SPME) during the post-exponential phase of growth and analyzed by GC-MS. [Result] 13 compounds were identified, 12 of which were sesquiterpenes with C15H24 formula and accounting for 99.62% of the complete compounds. Thujopsene-( 12), α-Guaiene and Aristolene were three most sesquiterpenes accounting for 10.66%, 33.69% and 34.85% of total content respectively. It can be sufficiently certified that sesquiterpene metabolic pathway existing in M. alpine. [ Coclusion] Metabolic flux of sesquiterpene pathway increased to improve its mass accumulation, because one or several key enzyme genes mutation in isoprene or sesquiterpene pathway enhanced their activities during induction of mutation from initial strain.展开更多
In this study, the influences of various environmental factors on the degradation of deoxynivalenol (DON) in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under differ...In this study, the influences of various environmental factors on the degradation of deoxynivalenol (DON) in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under different conditions for 150 d, DON toxin in wheat grains exhibited the degradation rate of 78.85%-87.00% and degradation half-life of 54.57-71.44 d. According to the accelerating effects of different conditions on DON degradation, natural light 〉 strong light (10 000 lx) 〉 darkness, 90% humidity 〉50% humidity, but high temperature could not accelerate the degradation of DON toxin.展开更多
Objective: To investigate the anti-proliferation e?ect of oridonin on leukemic NB4 cells and its mechanism. Methods: NB4 cells in culture medium in vitro were given di?erent concentrations of o...Objective: To investigate the anti-proliferation e?ect of oridonin on leukemic NB4 cells and its mechanism. Methods: NB4 cells in culture medium in vitro were given di?erent concentrations of oridonin. The inhibitory rate of the cells were measured by MTT assay, cell apoptotic rate was detected by ?ow cytometry(FCM), morphology of cell apoptosis was observed by hoechst 33258 ?uorescence staining , and the activity of telomerase was detected using TRAP-PCR-ELISA before and after apoptosis occurred. Results: Oridonin (over 8 μmol/L) could decrease the telomerase activity, inhibit the growth of NB4 cells and induce apoptosis signi?cantly in a time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed by hoechst 33258 ?uorescence staining especially after the cells treated by oridonin for 48–60 h. Conclusion: Oridonin could inhibit the proliferation and induce the apoptosis of NB4 cells in vitro. One of the mechanisms may be the decrease of the telomerase activity of NB4 cells.展开更多
A simple and facile gas chromatography-mass spectrometer (GC-MS) fingerprint of Su-He-Xiang-Wan (SHXW) was developed, the similarity analysis was conducted, and attribution of the major characteristic peaks was id...A simple and facile gas chromatography-mass spectrometer (GC-MS) fingerprint of Su-He-Xiang-Wan (SHXW) was developed, the similarity analysis was conducted, and attribution of the major characteristic peaks was identified for SHXW quality control. GC-MS analysis was performed on a QP2010 instrument (Shimadzu, Japan) equipped with a capillary column of RTX-5MS. The column temperature was initiated at 50℃, held for 5 min, increased at the rate of 3 ℃/min to 120 ℃, held for 2 min, and then increased at the rate of 4 ℃/min to 220℃, held for 10 min. Helium carrier gas was used at a constant flow rate of 1.3 mL/min. Mass conditions were ionization voltage, 70 eV; injector temperature, 250℃; ion source temperature, 250 ℃; splitting ratio, 30:1; full scan mode in the 40-500 Da mass ranges with rate of 0.2 s per scan. Attribution of the major characteristic peaks was identified for SHXW by comparing the chemical standards, references of Chinese herbal medicines and the negative controls of prescription samples (NC) of SHXW. With the help of the temperature-programmed retention indices (PTRIs) used together with mass spectra and chemical standards, 25 major characteristic peaks have been identified. Nine volatile medicinal materials were identified in the prescription of SHXW by attributing to the 27 major characteristic peaks. The results demonstrate that the proposed method is a powerful approach to quality control of complex herbal medicines.展开更多
Pesticide residues in fruit and vegetables have become a major concern associated with chemical food safety issues. Translocation of pesticides in tomato, mango and pineapple fruits in locally produced samples was inv...Pesticide residues in fruit and vegetables have become a major concern associated with chemical food safety issues. Translocation of pesticides in tomato, mango and pineapple fruits in locally produced samples was investigated using gas chromatography/mass spectrophotometer (GC/MS) procedures. Samples were fractionated with respect to distance from the skin to the core and prepared for analysis. Results showed that organochlorine, organophosphate and synthetic pyrethroid residues were translocated in all fruit samples analyzed. With respect to tomato fractions, the peels retained more residues compared to the pulp and the central core. In the chemical species, organochlorines were retained more in the peels of tomato than the other fractions of the fruit. More organophosphate and organochlorine residues were detected in pineapple peels compared to the pulp. Synthetic pyrethroid residues were evenly distributed in the pineapple fruit. In mangoes, the pulp retained more chemical residues than other fractions of the fruit. More organochlorine residues were retained in the pulp of mango than in the other fractions of the fruit. Synthetic pyrethroid residues were evenly distributed throughout the mango fruit. All the chemical species identified were translocatable across the fractional parts of all the fruits. There were significant differences in translocation and residue accumulation of pesticides among the fractional parts of all samples.展开更多
This paper describes design and calibration procedures of a new small-scale testing apparatus for evaluating soil-structure interaction. Two distinct modes of loadings, such as, direct shear and pullout loadings on fl...This paper describes design and calibration procedures of a new small-scale testing apparatus for evaluating soil-structure interaction. Two distinct modes of loadings, such as, direct shear and pullout loadings on flexible and rigid reinforcements are implemented by the multiple functionalities of the apparatus through the use of single loading frame, dividing mechanism, instrumentation and data acquisition systems. The design is similar to the conventional apparatus; however, it includes some key modifications to adapt additional features of reinforcements. For the pullout test, the top half of the pullout box is fixed with the bottom half of the pullout box, while for the direct shear test, the top half is allowed to slide horizontally on low friction roller bearings connected to the driving mechanism. Mechanical loadings through a steel plate are used to apply the normal load in the pullout and direct shear apparatus. A series of shear and pullout tests are performed to validate the functionality of the apparatus and to examine relationships between the reinforcements and its shear and pullout resistances.展开更多
Using the latest version of Mesoscale Modeling System (MMSv3), we assimilated wind data from the scatterometer and built a model to assimilate the wind field over eastern China seas and adjacent waters and applied t...Using the latest version of Mesoscale Modeling System (MMSv3), we assimilated wind data from the scatterometer and built a model to assimilate the wind field over eastern China seas and adjacent waters and applied the wave model WAVEWATCH-Ⅲ to test the sea area with assimilative wind and blended wind of QSCAT and NCEP as driving forces. High precision and resolution numerical wave results were obtained. Analysis indicated that if we replace the model wind result with the blended wind, better sea surface wind results and wave results could be obtained.展开更多
By using four specially designed narrow bandpass filters and photodetectors in the instrument, the incident and reflected radiances of sun light on the vegetation are optically sensed, at the red and near infrared ban...By using four specially designed narrow bandpass filters and photodetectors in the instrument, the incident and reflected radiances of sun light on the vegetation are optically sensed, at the red and near infrared bands, then the normalized difference vegetation index(NDVI) is processed by a microprocessor. Compared with conventional spectrometer measuring method of NDVI, the instrument is easy to be used, compact, light and low-cost.展开更多
Shell-feeding velocity is an important factor affecting naval gun shooting capacity. An agile shell-feeding system was designed to ensure quick implementation of the shell-feeding task. Based on composition of the agi...Shell-feeding velocity is an important factor affecting naval gun shooting capacity. An agile shell-feeding system was designed to ensure quick implementation of the shell-feeding task. Based on composition of the agile shell-feeding system, hoist technology was studied. Working principles were discussed and the hydraulic pressure system of the hoist was constructed. The hydraulic pressure cylinder and the accumulator were analyzed and calculated. Finally, PRO/E and ADAMS were used to simulate the hoist and its hydraulic system. It was found that this type of virtuaU674.7l prototype provides a good method to actualize a physical prototype.展开更多
基金Supported by the National Natural Science Foundation of China(20606018)the National Basic Research Program (also called 973Program)(2007CB707805)the Six Projects Sponsoring Talent Summits of Jiangsu Province~~
文摘[ Objective] The study was to investigate the volatile components of secondary metabolites from M. alpine producing arachidonic acid and explore the changes in its metabolic pathway. [ Method] The air above M. alpine broth was extracted by solid-phase microextraction(SPME) during the post-exponential phase of growth and analyzed by GC-MS. [Result] 13 compounds were identified, 12 of which were sesquiterpenes with C15H24 formula and accounting for 99.62% of the complete compounds. Thujopsene-( 12), α-Guaiene and Aristolene were three most sesquiterpenes accounting for 10.66%, 33.69% and 34.85% of total content respectively. It can be sufficiently certified that sesquiterpene metabolic pathway existing in M. alpine. [ Coclusion] Metabolic flux of sesquiterpene pathway increased to improve its mass accumulation, because one or several key enzyme genes mutation in isoprene or sesquiterpene pathway enhanced their activities during induction of mutation from initial strain.
基金Supported by the Youth Innovation Fund of President of Anhui Academy of Agricultural Sciences(13B1144)Key Science and Technology Project of Anhui Province(1301032138)+1 种基金Special Basic Research Fund(2013FY113400)Scientific and Technological Innovation Team Project of Anhui Academy of Agricultural Sciences(14C1105)~~
文摘In this study, the influences of various environmental factors on the degradation of deoxynivalenol (DON) in wheat grains were investigated using a triple quadrupole LC-MS/MS system. After being treated under different conditions for 150 d, DON toxin in wheat grains exhibited the degradation rate of 78.85%-87.00% and degradation half-life of 54.57-71.44 d. According to the accelerating effects of different conditions on DON degradation, natural light 〉 strong light (10 000 lx) 〉 darkness, 90% humidity 〉50% humidity, but high temperature could not accelerate the degradation of DON toxin.
文摘Objective: To investigate the anti-proliferation e?ect of oridonin on leukemic NB4 cells and its mechanism. Methods: NB4 cells in culture medium in vitro were given di?erent concentrations of oridonin. The inhibitory rate of the cells were measured by MTT assay, cell apoptotic rate was detected by ?ow cytometry(FCM), morphology of cell apoptosis was observed by hoechst 33258 ?uorescence staining , and the activity of telomerase was detected using TRAP-PCR-ELISA before and after apoptosis occurred. Results: Oridonin (over 8 μmol/L) could decrease the telomerase activity, inhibit the growth of NB4 cells and induce apoptosis signi?cantly in a time- and dose-dependent manner. Marked morphological changes of cell apoptosis were observed by hoechst 33258 ?uorescence staining especially after the cells treated by oridonin for 48–60 h. Conclusion: Oridonin could inhibit the proliferation and induce the apoptosis of NB4 cells in vitro. One of the mechanisms may be the decrease of the telomerase activity of NB4 cells.
基金Foundation item: Projects(21275164, 21075138) supported by the National Natural Science Foundation of China
文摘A simple and facile gas chromatography-mass spectrometer (GC-MS) fingerprint of Su-He-Xiang-Wan (SHXW) was developed, the similarity analysis was conducted, and attribution of the major characteristic peaks was identified for SHXW quality control. GC-MS analysis was performed on a QP2010 instrument (Shimadzu, Japan) equipped with a capillary column of RTX-5MS. The column temperature was initiated at 50℃, held for 5 min, increased at the rate of 3 ℃/min to 120 ℃, held for 2 min, and then increased at the rate of 4 ℃/min to 220℃, held for 10 min. Helium carrier gas was used at a constant flow rate of 1.3 mL/min. Mass conditions were ionization voltage, 70 eV; injector temperature, 250℃; ion source temperature, 250 ℃; splitting ratio, 30:1; full scan mode in the 40-500 Da mass ranges with rate of 0.2 s per scan. Attribution of the major characteristic peaks was identified for SHXW by comparing the chemical standards, references of Chinese herbal medicines and the negative controls of prescription samples (NC) of SHXW. With the help of the temperature-programmed retention indices (PTRIs) used together with mass spectra and chemical standards, 25 major characteristic peaks have been identified. Nine volatile medicinal materials were identified in the prescription of SHXW by attributing to the 27 major characteristic peaks. The results demonstrate that the proposed method is a powerful approach to quality control of complex herbal medicines.
文摘Pesticide residues in fruit and vegetables have become a major concern associated with chemical food safety issues. Translocation of pesticides in tomato, mango and pineapple fruits in locally produced samples was investigated using gas chromatography/mass spectrophotometer (GC/MS) procedures. Samples were fractionated with respect to distance from the skin to the core and prepared for analysis. Results showed that organochlorine, organophosphate and synthetic pyrethroid residues were translocated in all fruit samples analyzed. With respect to tomato fractions, the peels retained more residues compared to the pulp and the central core. In the chemical species, organochlorines were retained more in the peels of tomato than the other fractions of the fruit. More organophosphate and organochlorine residues were detected in pineapple peels compared to the pulp. Synthetic pyrethroid residues were evenly distributed in the pineapple fruit. In mangoes, the pulp retained more chemical residues than other fractions of the fruit. More organochlorine residues were retained in the pulp of mango than in the other fractions of the fruit. Synthetic pyrethroid residues were evenly distributed throughout the mango fruit. All the chemical species identified were translocatable across the fractional parts of all the fruits. There were significant differences in translocation and residue accumulation of pesticides among the fractional parts of all samples.
文摘This paper describes design and calibration procedures of a new small-scale testing apparatus for evaluating soil-structure interaction. Two distinct modes of loadings, such as, direct shear and pullout loadings on flexible and rigid reinforcements are implemented by the multiple functionalities of the apparatus through the use of single loading frame, dividing mechanism, instrumentation and data acquisition systems. The design is similar to the conventional apparatus; however, it includes some key modifications to adapt additional features of reinforcements. For the pullout test, the top half of the pullout box is fixed with the bottom half of the pullout box, while for the direct shear test, the top half is allowed to slide horizontally on low friction roller bearings connected to the driving mechanism. Mechanical loadings through a steel plate are used to apply the normal load in the pullout and direct shear apparatus. A series of shear and pullout tests are performed to validate the functionality of the apparatus and to examine relationships between the reinforcements and its shear and pullout resistances.
基金Supported by the High-Tech Research and Development Program of China (863 Program, No. 2001AA633070 2003AA604040)the National Natural Science Foundation of China (No. 40476015).
文摘Using the latest version of Mesoscale Modeling System (MMSv3), we assimilated wind data from the scatterometer and built a model to assimilate the wind field over eastern China seas and adjacent waters and applied the wave model WAVEWATCH-Ⅲ to test the sea area with assimilative wind and blended wind of QSCAT and NCEP as driving forces. High precision and resolution numerical wave results were obtained. Analysis indicated that if we replace the model wind result with the blended wind, better sea surface wind results and wave results could be obtained.
文摘By using four specially designed narrow bandpass filters and photodetectors in the instrument, the incident and reflected radiances of sun light on the vegetation are optically sensed, at the red and near infrared bands, then the normalized difference vegetation index(NDVI) is processed by a microprocessor. Compared with conventional spectrometer measuring method of NDVI, the instrument is easy to be used, compact, light and low-cost.
文摘Shell-feeding velocity is an important factor affecting naval gun shooting capacity. An agile shell-feeding system was designed to ensure quick implementation of the shell-feeding task. Based on composition of the agile shell-feeding system, hoist technology was studied. Working principles were discussed and the hydraulic pressure system of the hoist was constructed. The hydraulic pressure cylinder and the accumulator were analyzed and calculated. Finally, PRO/E and ADAMS were used to simulate the hoist and its hydraulic system. It was found that this type of virtuaU674.7l prototype provides a good method to actualize a physical prototype.