A MADS box gene （GhMADS3） was cloned from cotton （Gossypium hirsutum L.） based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum m...A MADS box gene （GhMADS3） was cloned from cotton （Gossypium hirsutum L.） based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum majus FAR, and Arabidopsis thaliana AG, respectively, and was grouped with AG homologues when the full length sequences excluding N-extensions were compared. GhMADS3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to AG in Arabidopsis. However, it was not expressed in floral buds of a homeotic cotton variant chvl. Ectopic expression of GhMADS3 in tobacco （Nicotiana tabacum L.） resulted in flowers with sepal-to-carpel and petal-to-stamen transformation. The carpelloid first whorl organs, with stigmatic tissue on their upper edges, had a white appearance when compared with the dark green color of the wild type sepals. At times, long filaments were observed at the fusion site of the first carpelloid oranges. The second whorl organs in staminoid were usually smaller than the wild type and the color was changed from pink to white. These results suggest that GhMADS3 has a homeotic role in flower development.展开更多
Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic mater...Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic material, promoting blood circulation and removing blood stasis'.It is a well known Chinese folk-medicine used for the treatment of appendicitis, sore throat, mumps,acne, sebo-rheic dermatitis and various kinds of tumors, such as tumors of digestive tract,carcinoma of liver. It was reported that the MeOH extract of H. diffusa demonstrated a significantantitumor activity and ursolic acid succeeded in being isolated from the MeOH extract as an activecomponent . Shan BN, et al suggested that the direct aqueous extract of H. diffusa hadimmuno-modulating activity and antitumor activity in vitro through stimulating the immune system tokill or engulf tumor cells. But regarding anti-tumor activity in vivo of water soluble extracts fromH. diffusa, no detail was reported. Therefore, we prepared water soluble extracts (H_1 and H_2)from H. diffusa and evaluated their anti-tumor property in vivo experiments as well as protectiveeffect on chemo-therapeutic damage.展开更多
[Objective] The aim was to explore the production potential and extension value of Yun peanut No.3 to lay foundation for further extension. [Method] Selection, breeding, characters, yield and the stability of Yun pean...[Objective] The aim was to explore the production potential and extension value of Yun peanut No.3 to lay foundation for further extension. [Method] Selection, breeding, characters, yield and the stability of Yun peanut No.3 were analyzed. [Result] Yun peanut No.3 is a new early-mature peanut species bred based on foreign resources and local varieties through sexual hybridization and improvement. It contains 50.32% of fat, 26.5% of protein, 38.93% of oleic acid, 38.88% of linoleic acid, and the ratio of oleic acid and linoleic acid is at 1.00. It is a typical small-scale peanut with red skin, high contents of oil and fat, and constitutes the first one approved by cultivar registration in China. According to related tests, pod yield achieved 3 615.9 kg/hm2 ; kernel yield was 2 570.1 kg/hm2 and oil output was 1 293.3 kg/hm2 . For the production at scale, pod yields would be about 3 000 kg/hm2 ; the production value of fruit would be 25 000 Yuan/hm2 ; the yield per unit area would be maximal at 16 000 kg/hm2 , totaling 64 000 Yuan/hm2 . It enjoys different excellent qualities, such as drought-resistance and barren-resistance. It was approved in Yunnan Province in 2012 and extended at 20 000 Yuan/hm2 in major production lands. [Conclusion] Yun peanut No.3, which is highly-yield and highly-qualified, is suitable to be grown in Yunnan, as well as similar conditions in southern regions in China.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.30070485)Southwest University InitialResearch Foundation Grant to Doctor(No.D200404).
文摘A MADS box gene （GhMADS3） was cloned from cotton （Gossypium hirsutum L.） based on EST sequences. The predicted protein sequence of GhMADS3 showed 85%, 73%, and 62% identity with Theobroma cacao TcAG, Antirrhinum majus FAR, and Arabidopsis thaliana AG, respectively, and was grouped with AG homologues when the full length sequences excluding N-extensions were compared. GhMADS3 expressed in the wild type cotton flower primarily in stamens and carpels, which was comparable to AG in Arabidopsis. However, it was not expressed in floral buds of a homeotic cotton variant chvl. Ectopic expression of GhMADS3 in tobacco （Nicotiana tabacum L.） resulted in flowers with sepal-to-carpel and petal-to-stamen transformation. The carpelloid first whorl organs, with stigmatic tissue on their upper edges, had a white appearance when compared with the dark green color of the wild type sepals. At times, long filaments were observed at the fusion site of the first carpelloid oranges. The second whorl organs in staminoid were usually smaller than the wild type and the color was changed from pink to white. These results suggest that GhMADS3 has a homeotic role in flower development.
文摘Bai-Hua-She-She-Cao Hedyotis diffusa Willd. (Ru-biaceae) is a medicinal herbwidely distributed in northeast Asian countries. In traditional Chinese medicine, it has the effectof 'clearing away heat and toxic material, promoting blood circulation and removing blood stasis'.It is a well known Chinese folk-medicine used for the treatment of appendicitis, sore throat, mumps,acne, sebo-rheic dermatitis and various kinds of tumors, such as tumors of digestive tract,carcinoma of liver. It was reported that the MeOH extract of H. diffusa demonstrated a significantantitumor activity and ursolic acid succeeded in being isolated from the MeOH extract as an activecomponent . Shan BN, et al suggested that the direct aqueous extract of H. diffusa hadimmuno-modulating activity and antitumor activity in vitro through stimulating the immune system tokill or engulf tumor cells. But regarding anti-tumor activity in vivo of water soluble extracts fromH. diffusa, no detail was reported. Therefore, we prepared water soluble extracts (H_1 and H_2)from H. diffusa and evaluated their anti-tumor property in vivo experiments as well as protectiveeffect on chemo-therapeutic damage.
基金Supported by Yunnan Key New Cultivar Planting Program (2011BB010)National Penaut Industry Technology System (CARS-14)~~
文摘[Objective] The aim was to explore the production potential and extension value of Yun peanut No.3 to lay foundation for further extension. [Method] Selection, breeding, characters, yield and the stability of Yun peanut No.3 were analyzed. [Result] Yun peanut No.3 is a new early-mature peanut species bred based on foreign resources and local varieties through sexual hybridization and improvement. It contains 50.32% of fat, 26.5% of protein, 38.93% of oleic acid, 38.88% of linoleic acid, and the ratio of oleic acid and linoleic acid is at 1.00. It is a typical small-scale peanut with red skin, high contents of oil and fat, and constitutes the first one approved by cultivar registration in China. According to related tests, pod yield achieved 3 615.9 kg/hm2 ; kernel yield was 2 570.1 kg/hm2 and oil output was 1 293.3 kg/hm2 . For the production at scale, pod yields would be about 3 000 kg/hm2 ; the production value of fruit would be 25 000 Yuan/hm2 ; the yield per unit area would be maximal at 16 000 kg/hm2 , totaling 64 000 Yuan/hm2 . It enjoys different excellent qualities, such as drought-resistance and barren-resistance. It was approved in Yunnan Province in 2012 and extended at 20 000 Yuan/hm2 in major production lands. [Conclusion] Yun peanut No.3, which is highly-yield and highly-qualified, is suitable to be grown in Yunnan, as well as similar conditions in southern regions in China.
