八肽胆囊收缩素对抗电针对大鼠尾核痛反应神经元电活动和甩尾痛阈的同时影响

Objective: To study the influence of intra cerebroventricular injection of cholecystokinin octapeptide (CCK 8) on the effect of electroacupuncture (EA) i n antagonizing the electrical activity of pain related neurons in caudate nucle us (Cd) and raising pain threshold.Methods: 30 male Wistar rats were used. Operations were performed under general anesthesia with 20% urethane (1.0 g/kg of body weight). The radiant heat irradiation (nociceptive stimulus) induce d rat tail flick reaction was used as the pain index. Extracellular discharges o f pain related neurons pain excitatory neurons (PEN) and pain inhibitory ne urons (PIN) in Cd and tail flick latency (TFL) before and after cerebroventri cular microinjection of CCK 8 (15 ng) were recorded. Electroacupuncture (EA) wa s applied to bilateral "Zusanli" (ST 36).Results: ① The radiant heat focused on the tail of rats caused increase of the pain evoked discharging frequency and shortening of the evoked discharging latency of PEN or reduction of the pain evoked discharging frequency and prolongation of the inhibitory du ration of the evoked discharges of PIN and generated tail flick reflex simultaneously. ② EA of bilateral "Zusanli" (ST 36) for 15 min resulted in inh ibition of the electric activity of PEN as well as potentiation of the electrica l activity of PIN and a prolongation of tail flick latency (TFL), i.e. exhibitin g the analgesic effect of EA. The effects peaked immediately after EA, the net i ncrease value (NIV) of the pain evoked discharges of 19 PENs was reduced from 1 6.17±2.30 Hz to 5.45±2.96 Hz and TFL was prolonged from 5.03±0.31 sec to 8.89 ±0.58 sec simultaneously, the inhibitory duration of the pain evoked discharge s of 12 PINs was shortened from 5.19±0.24 sec to 2.52±0.33 sec and TFL was pro longed from 4.57±0.23 sec to 8.12±0.29 sec simultaneously. These changes recov ered gradually 10 min after EA. ③ The inhibitory effect of EA on the pain evok ed discharges of PEN and the potentiated effect of EA on the electric activities of PIN, and the prolonged effect of EA on TFL were antagonized by intra cerebr oventricular injection of 15 ng CCK 8, i.e. CCK 8 could antagonize the analges ic effect of EA. About 4 min after injection of CCK 8, the effects were most ap parent. Very soon after EA, the NIV of 13 PENs was reduced from 9.36±2.10 Hz to 2.34±0.46 Hz and TFL was prolonged from 5. 38±0.18 sec to 8.60±0.49 sec simu ltaneously, the inhibitory duration of 10 PINs was shortened from 5.54±0.32 sec to 2.09±0.79 sec and TFL was prolonged from 4.92±0.17 sec to 9.44±0.21 sec s imultaneously. About 4 min after injection of CCK 8, the NIV of PENs was recov ered to 7.44± 1.38 Hz and TFL to 5.53±0.19 sec, the inhibitory duration of PINs was recovered to 6.20± 0.61 sec and TFL to 4.54±0.16 sec. About 14 min after injection of CCK 8, it gradually recovered. Conclusion: T he results demonstrate that the antagonism of CCK 8 on analgesic effect of EA s hows a coordinated and consistent action at the levels of electrical activity of central neurons and the whole behavior reaction.

灸法镇痛效应的观察

聚光灯幅射热照射次髎穴,穴位表面温度分别调节为38～39℃和43～44℃,照射5分钟后鼠甩尾阈提高17.8±2.1%和22.2±2.5%,照射10分后提高16.1±2.9%,22.1±3.4%和21.9±3.2%(50～52℃)。10分的针上加灸提高19.8±3.1%。各组的甩尾阈在照射前后的变化有统计学差异(P<0.05),但各组间效应相比无统计学差异(P>0.05)。幅射热灸次髎穴的效应强于灸足三里和至阳穴(P<0.05)。

蕲艾挥发油的抗炎、抗过敏和镇痛作用

目的研究蕲艾挥发油的抗炎、抗过敏和镇痛作用。方法采用二甲苯致炎法,细菌致菌法,2,4二硝基氯苯致敏法,小鼠热板法,小鼠扭体法,大鼠甩尾法,大鼠子宫镇痛法等方法。结果蕲艾挥发油能够明显抑制二甲苯引起的小鼠耳壳炎症,抑制金黄葡萄球菌,绿脓杆菌,大肠杆菌,变形杆菌等细菌生长;抑制2,4二硝基氯苯诱导的迟发性超敏反应;小鼠热板反应潜伏期延长,抑制小鼠扭体次数,能提高大鼠甩尾痛阈;对抗己烯雌酚和缩宫素引起的大鼠子宫收缩作用。结论实验结果表明,蕲艾挥发油具有明显的抗炎、抗过敏和镇痛作用。

气功麻醉机理初步探讨——外气镇痛效应的研究

目的:为探讨气功麻醉机理,首先设计实验初步观察外气对家兔、大鼠及在人体的镇痛效应。方法:用 WQ-9E 痛阈测量仪发生的缓慢递升直流电使钾离子透入家兔耳尖部皮肤致痛,以甩头阈为指标,又用同一仪器发生的直流电刺激鼠尾,以甩尾阈及头动阈为指标,观察气功师对动物发放外气时及停止放气后上述痛反应指标的变化;在人体亦用这仪器使钾离子透入颈部皮肤致痛,观察气功师对其发放外气时痛阈及耐痛阈是否提高;另在大鼠观察牙髓刺激诱发的皮层电位在外气作用下的变化。结果:在家兔及大鼠的行为学实验及大鼠电生理学实验均未证明外气的镇痛效应;在人体,外气对实验痛亦无明显影响,同体比较外气对实验痛的作用与手术时气功麻醉效果,未见二者之间有明显相关关系。结论:本实验结果不能令人满意;看来,气功麻醉机理仍需进行认真、仔细的探索。