Aberrant tumor suppressor gene promoter methylation was associated with the several cancers, including breast cancer, which was the common female deaths in most countries involved in Vietnam. The methylation in tumor ...Aberrant tumor suppressor gene promoter methylation was associated with the several cancers, including breast cancer, which was the common female deaths in most countries involved in Vietnam. The methylation in tumor suppressor genes, including RASSFIA, were the key targets of establishing the potential biomarkers for prognosis and early diagnosis of breast cancer. In present study, with the aim towards using the hypermethylation at CpG islands of promoter of RASSFIA as the biomarker for breast cancer in Vietnamese population, MSP （methyl specific PCR） was carried out to analyze the hypermethylation status ofRASSFIA gene in 115 samples including 95 breast cancer specimens and 20 normal breast tissues from another disease （not breast cancer）. All samples were obtained from Ho Chi Minh City Medical Hospital, Vietnam. The known predictive and prognostic factors： HER2/neu overexpression was immunohistochemistry stained as input value for breast cancer specimens. For input value confirmed, the overexpression of p53 protein was also analyzed together with prior immunochemical assay. The results indicated that the hypermethylation of frequencies for methylation of given gene reached 42.1% （P 〈 0.05）. In addition, the DNA hypermethylation of RASSFIA gene increased the possibility to be breast cancer with high incidence via calculated of odd ratio （P 〈 0.05）. In conclusion, the hypermethylation of candidate genes could be used as the promising biomarkers applying in Vietnamese breast cancer patients.展开更多
文摘Aberrant tumor suppressor gene promoter methylation was associated with the several cancers, including breast cancer, which was the common female deaths in most countries involved in Vietnam. The methylation in tumor suppressor genes, including RASSFIA, were the key targets of establishing the potential biomarkers for prognosis and early diagnosis of breast cancer. In present study, with the aim towards using the hypermethylation at CpG islands of promoter of RASSFIA as the biomarker for breast cancer in Vietnamese population, MSP （methyl specific PCR） was carried out to analyze the hypermethylation status ofRASSFIA gene in 115 samples including 95 breast cancer specimens and 20 normal breast tissues from another disease （not breast cancer）. All samples were obtained from Ho Chi Minh City Medical Hospital, Vietnam. The known predictive and prognostic factors： HER2/neu overexpression was immunohistochemistry stained as input value for breast cancer specimens. For input value confirmed, the overexpression of p53 protein was also analyzed together with prior immunochemical assay. The results indicated that the hypermethylation of frequencies for methylation of given gene reached 42.1% （P 〈 0.05）. In addition, the DNA hypermethylation of RASSFIA gene increased the possibility to be breast cancer with high incidence via calculated of odd ratio （P 〈 0.05）. In conclusion, the hypermethylation of candidate genes could be used as the promising biomarkers applying in Vietnamese breast cancer patients.
