基于自我决定理论的护理对维持性血液透析继发甲旁亢患者生活质量改善情况的影响

探究自我决定理论的护理对维持性血液透析继发甲旁抗患者生活质量改善情况。方法 以我院近三年收治的维持性血液透析继发甲旁抗患者为研究对象,随机分组,常规护理对照组,自我决定理论护理观察组,对比两组生活质量改善情况。结果 干预后,观察组患者生活质量明显较高,生化指标改善显著,自我管理得分较高(P<0.05)。结论 基于自我决定理论的护理对维持性血液透析继发甲旁亢患者应用,可改善患者生活质量、生化指标等,效果显著。

不同剂量的骨化三醇冲击治疗血液透析继发甲旁亢的疗效分析

目的探讨骨化三醇冲击治疗血液透析继发甲旁亢的疗效。方法选取2013年11月到2015年3月我院收取的85例继发甲旁亢的血液透析病人,并按照随机数字表法分为对照组(n=42)和冲击组(n=43)。根据i PTH值,再将对照组和冲击组分为2个亚组,即对照组1、冲击组1(300pg/m L-600pg/m L),对照组2、冲击组2(>600pg/m L)。对照组1和对照组2均行常规治疗:口服骨化三醇,0.5μg/次,1次/d。冲击组1、冲击组2分别给予病人口服骨化三醇,2.0μg/次,2次/周和2.0μg/次,3次/周。对比各组治疗效果。结果冲击组4周、8周和12周治疗达标率分别为20.9%(9/43)、30.2%(13/43)、46.5%(20/43),显著高于对照组的0%(0/42)、2.4%(1/42)、4.8%(2/42)(P<0.05)。与治疗前相比,对照组和冲击组治疗4周、8周和12周的i PTH值显著降低(P<0.05);与对照组1相比,冲击组1治疗4周、8周和12周的i PTH值更低(P<0.05);与对照组2相比,冲击组2治疗治疗4周、8周和12周的i PTH值更低(P<0.05)。冲击组、对照组不良反应相比,差异无统计学意义(P>0.05)。结论骨化三醇冲击治疗能够有效地改善血液透析继发甲旁亢病情,治疗达标率高,可有效地降低i PTH值。

溯源性心理疏导对血液透析继发性甲状旁腺功能亢进患者的影响

目的探讨溯源性心理疏导对手术切除甲状旁腺治疗血液透析继发性甲状旁腺功能亢进(血液透析继发性甲旁亢)患者预后的影响。方法选取2017年6月至2019年10月我院收治的80例血液透析继发性甲旁腺亢患者,按随机数字表法分为试验组和对照组各40例。对照组采用常规护理,观察组在常规护理基础上采用溯源性心理疏导,比较两组患者的护理效果。结果观察组患者疾病相关知识掌握率、生活质量各维度评分以及护理依从率均明显高于对照组(P<0.05),而焦虑自评量表(SAS)和抑郁自评量表(SDS)评分低于对照组(P<0.05)。结论溯源性心理疏导能够有效提高血液透析继发性甲旁亢患者的心理状态、疾病认知水平以及生活质量,可在临床中推广。

TLC法测定柴黄胃宁颗粒中黄芪甲苷的含量

目的建立柴黄胃宁颗粒的中黄芪甲苷的含量测定方法.方法以氯仿-甲醇-水（13∶ 6∶ 2）10℃以下放置过夜的下层溶液为展开剂,以10%硫酸乙醇液浸板,在100℃加热5～7 min至斑点显色清晰.在530 nm和700 nm处双波长反射式锯齿形扫描,建立薄层扫描测定法.结果黄芪甲苷的量在1.0～5.2 μg范围内呈良好的线性关系,r=0.996,平均回收率为97.09%,RSD=1.42%.结论薄层扫描法操作简便、快捷、重现性好.

圆弧甲板梁拱曲线绘制方法

甲板梁拱曲线以往多采用摆线或抛物线,在放样时通过作图法来求得。由于各点的曲率是变化的,给制造工艺带来不便。现今大都采用圆弧曲线作为甲板梁拱曲线,采用计算机放样比较方便。本文介绍圆弧甲板梁拱曲线的手工绘制方法。圆弧甲板梁拱曲线是由船中剖面的甲板宽MN和梁拱高度OK,通过MKN三点作圆弧而获得(图1)。即使是船宽4米的小船,其圆弧直径也在50米以上。

Flow injection chemiluminescence determination of meloxicam using potassium permanganate and formaldehyde system

A simple, rapid and sensitive flow injection chemiluminescence （FI-CL） method has been developed for the determination of meloxicam. The method is based on the CL-emitting reaction between meloxicam and potassium permanganate in a hydrochloric acid medium, enhanced by formaldehyde （HCHO）. Under optimum conditions, calibration curve over the range of 1.0-20.0μg/mL was obtained. The proposed method was successfully applied to the determination of meloxicam in capsules with no evi- dence of interference from common excipients. The detection limit of this method was 25.6 ng/mL. The relative standard deviation was 2.1% for 10.0 μg/mL meloxicam. The sample throughput was found to be 120 samples/h.

Composition Analysis on Intermuscular Fatty Acid of Qinghai Datong Yak

[ Objective] The study aimed to provide a theoretical basis for rational utilization of Datong yak resource. [ Method] By qualitative and quantitative determination with gas chromatograph, the composition and content of intermuscular fatty acid of Datong yak were compared with those of Datong yellow cattle. [ Result] Comparing with Datong yellow cattle, Datong yak had lower saturated fatty acid （SFA） content and higher polyunsaturated fatty acid （PUFA） content at extremely significant level （P 〈0.01 ）, while the difference of monounsaturated fatty acid （MUFA） contents between them was not significant （P〉0.05）. The PUFA/SFA and n-6/n-3 PUFA of Datong yak were in accordance with the recommended proportions. [ Conclusion] The intermuscular fatty acid composition of Datong yak is superior to that of local yellow cattle, which indicates that Datong yak meat has high nutritive value and wide development prospect.

Differential roles of EPS8 in carcinogenesis:Loss of protein expression in a subset of colorectal carcinoma and adenoma

AIM:To analyze the epidermal growth factor receptor pathway substrate 8(EPS8) expression status and role in colorectal carcinogenesis given that EPS8 has a conserved actin barbed-end capping function that is required for proper maturation in intestinal cells.METHODS:We studied 8 colon cancer cell lines and 58 colorectal tumors(19 adenomas and 39 carcinomas).We performed expression microarray analysis of colon cancer cell lines followed by loss of heterozygosity(LOH)analysis and immunohistochemistry for EPS8 expression in colon tumors.Subsequently,we performed mutation analysis by direct sequencing and methylation analysis by bisulfite sequencing and methylation-specific polymerase chain reaction assays.RESULTS:Expression microarray analysis of colon cancer cell lines showed overexpression of EPS8 transcript in all lines but RKO.Genome wide loss of heterozygosity(LOH) analysis of colon tumors,showed considerable LOH at the EPS8 gene locus.Immunohistochemically,EPS8 was constitutively expressed in normal colonic mucosa with a dot-like supranuclear localization with accentuation at the luminal surface supporting its proposed role in epithelial maturation.Nineteen colon tumors(4 adenoma,15 carcinoma) out of 51(37%) showed strikingly tumor specific EPS8 protein loss.Of the remaining tumors,5/51(2 adenoma,and 3 carcinoma,10%) showed marked overexpression,while 27/51 tumors(53%) showed retained expression.Mutation analysis revealed a missense mutation(c.794C>T,p.R265C) in exon 8 in RKO.The EPS8 promoter was also methylated in RKO,but there was no significant methylation in other cell lines or carcinoma specimens.CONCLUSION:The loss of EPS8 expression in colorectal adenomas and carcinomas suggests that down regulation of this gene contributes to the development of a subset of colorectal cancers,a finding which could have applications in diagnosis and treatment.

Proteomic Analyses of the Shrimp White Spot Syndrome Virus

White spot syndrome virus (WSSV), a unique member within the virus family Nimaviridae, is the most notorious aquatic virus infecting shrimp and other crustaceans and has caused enormous economic losses in the shrimp farming industry worldwide. Therefore, a comprehensive understanding of WSSV morphogenesis, structural proteins, and replication is essential for developing prevention measures of this serious parasite. The viral genome is approximately 300kb and contains more than 180 open reading frames (ORF). However, most of proteins encoded by these ORF have not been characterized. Due to the importance of WSSV structural proteins in the composition of the virion structure, infection process and interaction with host cells, knowledge of structural proteins is essential to understanding WSSV entry and infection as well as for exploring effective prevention measures. This review article summarizes mainly current investigations on WSSV structural proteins including the relative quantities, localization, function and protein-protein interactions. Traditional proteomic studies of 1D or 2D gel electrophoresis separations and mass spectrometry (MS) followed by database searches have identified a total of 39 structural proteins. Shotgun proteomics and iTRAQ were initiated to identify more structural proteins. To date, it is estimated that WSSV is assembled by at least 59 structural proteins, among them 35 are defined as the envelope fraction (including tegument proteins) and 9 as nucleocapsid proteins. Furthermore, the interaction within several major structural proteins has also been investigated. This identitification and characterization of WSSV protein components should help in the understanding of the viral assembly process and elucidate the roles of several major structural proteins.

Checkpoint with forkhead-associated and ring finger promoter hypermethylation correlates with microsatellite instability in gastric cancer

AIM:To examine the methylation status of the promoter region of the checkpoint with forkhead-associated and ring fi nger(CHFR) and microsatellite mutator status in 59 primary gastric cancers.METHODS:We investigated the promoter methylation of CHFR in 59 cases of gastric cancer using methylation-specifi c PCR.Five microsatellite loci were analyzed using high-intensity microsatellite analysis reported previously, and p53 gene mutations were investigated by direct sequencing.RESULTS:Twenty cases(33.9%) showed promoter methylation and no relation was observed with the clinicopathological factors.We found that the promoter methylation of CHFR was frequently accompanied with microsatellite instability(MIN).Seven of 20(35.0%) cases showed MIN in hypermethylation of the CHFR tumor, while three of 39(7.7%) cases showed MIN in the non-methylated CHFR tumor(P < 0.01).However, we failed to fi nd any relationship between CHFR methylation and p53 mutation status.CONCLUSION:The coordinated loss of both the mitotic check point function and mismatch repair system suggests the potential to overcome the cell cycle check point, which may lead to an accumulation of mutations.However, the p53 mutation was not related to hypermethylation of the CHFR promoter and MIN, which indicates that an abnormality in p53 occurs as an independent process from the mismatch repair deficiency in carcinogenesis.

Simulation analysis of ammonia distribution in methanol production from coal water slurry gasification

Aspen plus software was employed to simulate process. The system concludes gasification scrubbing system the opposed multi-burner gasifier （OMB） methanol production and purification shift system. The distributions of ammonia con- centration in streams were obtained. The study demonstrates that ammonium crystallization problem caused by ammonia ac- cumulation, and if the process has ammonia exports its concentration will greatly reduced and the ammonia salt problem will effectively alleviate. Aspen plus simulation is a useful tool strengthening the ammonia recycling use and reducing pollutant for improving water quality, maintaining stable production, emissions.

Study on Morphology and Non-isothermal Crystallization Behavior of Poly(phenylene sulfide)/Poly(ethylene-co-cyclo-hexane 1,4-dimethanol terephthalate) Blend

A binary alloy consisting of poly（phenylene-sulfide） （PPS）/poly（ethylene terephthalate-co-l,4- cyclohexanedimethanol） （PETG） was prepared by the melt blending technology using a twin-screw extruder. The morphology and crystallization behavior of the alloy material were investigated by means of SEM, POM and DSC. The SEM study of the alloy samples revealed that PPS and PETG comprised an incompatible system and the interface structure of two phases could be observed distinctly when the composition of the binary alloy was being changed. The POM results had revealed that incorporation of PETG into PPS could lead to formation of larger spherulite crystals in the course of PPS crystallization, but small and grainy spherulite crystals appeared with further increase in the PETG concentration. The DSC analyses revealed that addition of PETG to the alloy composition could shift the PPS crystallization temperature towards the high-temperature region.

Anti-Hepatitis B Virus Drugs in Clinical and Preclinical Development

Up to date, there are two types of drugs approved to treat hepatitis B： interferons and nucleos （t） ide analogues. However, the therapies are limited in the clinical context because of the negative side effects of interferon-or and the development of substantial viral resistance to nucleos （t） idic inhibitors. Therefore, new drugs with novel structures and mechanisms are needed. In this article, the drugs approved by FDA or the European Commission for treating chronic hepatitis B virus infection, as well as those under clinical trials, and several compounds in preclinical studies are reviewed. Additionally, some potential targets and strategies to combat chronic hepatitis B virus infection are discussed.

Numerical analysis of methane migration from floor strata to a longwall face

A two-dimensional numerical mine strata in the vicinity of seam 349 in model of a large （1 000 m× 200 m） section of Iongwall panel 802 in part B of the Murcki Colliery in the Upper Silesian Coal Basin, Poland, was built using the Universal Distinct Element Code UDEC. Longwall extraction of seam 349 with roof caving was simulated in the model over a length of 450 m. Mining-induced changes in displacements, strains and stresses in the mine strata were investigated. Under the assumptions that （1） methane-bearing strata occur 15.6 m beneath seam 349 and include seam 350 and the strata lying beneath, and （2） the methane reservoir pressure is equal to 2 MPa, a fully coupled mechanical hydraulic analysis was performed in which joint conductivity was dependent on the mechanical deformation and, conversely, the mechanical behavior of rock masses was affected by joint fluid pressure. It was shown that migration of methane from coal seams lying beneath the mined seam is possible under conditions where the floor strata deform to a great extent, undergo separation, fracture and break into blocks.

Two new steroidal alkaloids from Veratrum nigrum var.ussuriense

Two new steroidal alkaloids, veraussines A （1） and B （2） were isolated from the roots and rhizomes of Veratrum nigrum var. ussuriense. Their structures were determined as N-（ethoxycarbonyl）- 1 α,2β,3α, 15α-tetrahydroxy-5fl-jervanin- 12-en- 11 -one （1） and N-（methoxycarbonyl）-1α,2β,3α,15α-tetrahydroxy-5/3-jervanin-12-en-1 l-one （2） by spectroscopic analysis.

DNA Methylation Analysis of Cassava （Manihotesculenta Crantz） SC8 and Its Autotetraploid in Response to Cold Stress

DNA methylation plays a vital role in the regulation of gene expression in response to environmental stress. However, little is known about the effect of DNA methylation on the cassava polyploidy. In the present study, methylation-sensitive amplified polymorphisms （MSAP） were used to investigate DNA methylation profiles of cassava polyploidy following cold treatment to identify candidate genes involved in response to cold stress. The result showed that the genome-wide DNA methylation polymorphisms accounted for 34.02%-42.56% in SC8 and its autotetraploid exposed to 5 ~C for 2, 8, 24 and 48 h, respectively. The methylation levels of SC8 at 2 h-cold stress were the highest during 48h under cold treatments. With the time extension within 48 h under cold stress, the methylation levels gradually decreased to the same level as the control but DNA methylation levels of cassava autotetraploid were stable within 48 h. For future analysis of the methylation extent, the cold stress induced more DNA methylation than demethylation in SC8, where DNA methylation was consistent with demethylation in its autotetraploid. The expression analysis demonstrated increase in the transcription of one methylated gene and decrease in the transcription of two demethylated genes. The results revealed that gene methylations in specific sites would be a rapidly epigenetic response to cold stress, further elucidating the methylation functions in its autotetraploid.

Adsorption Behavior of CH2 and CH3 on Metal Clusters CUn （n=1-6）

Using density functional theory with generalized gradient approximation and hybrid functional, we studied the properties of energy, charge population, and vibration of CH2 and CH3 adsorbed on Cun （n=1-6） clusters. The results show that the DFT calculation with the hybrid functional matches the experimental results better in both cases. The calculation results indicate that the adsorption of CH2 is stronger than that of CH3. During adsorption, the charges transfer from Cu to CH2 or CH3. The obtained vibrational frequencies for different modes of CH2 and CH3 adsorbed on Cun agree well with the experimental results for the adsorption on Cu（111） surface.

Photodissociation Dynamics of Methanol and Ethanol at 157 nm

157 nm photodissociation of jet-cooled CH3OH and C2HsOH was studied using the high-n Rydberg atom time-of-flight （TOF） technique. TOF spectra of nascent H atom products were measured. Simulation of these spectra reveals three different atomic H loss processes： one from hydroxyl H elimination, one from methyl （ethyl） H elimination, and one from secondary dissociation of the methoxy （ethoxy） radical. The relative branching ratio indicates secondary dissociation of ethoxy is less important than that of methoxy. The average angular anisotropy parameter of methanol is negative （with β≈-0.3）, indicating the transition dipole moment is perpendicular to the C-O-H plane. The slightly more negative β value of ethanol （with β≈-0.4） implies that ethanol has a longer rotational period. These experimental results indicate that both systems undergo fast internal conversion to the 3s surface after it is excited to the 3px surface, and then dissociate on the 3s surface. The translational energy distribution of the CH3O＋H products reveals extensive CH3 rocking or CH3 umbrella excitation in the CH30 radical. However the vibrational structures are not resolved in the C2H5O radical.

MULTIVARIATE ANALYSIS OF RELATIONSHIPS BETWEEN IODINE BIOLOGICAL EXPOSURE AND SUBCLINICAL THYROID DYSFUNCTIONS

Objective To assess the relationships between iodine biological exposure and subclinical thyroid dysfunctions. Methods The cross-sectional survey was performed to obtain the epidemiologic data of population in three communities with different iodine biological exposure： mild iodine deficiency [median urinary iodine concentration （MUI） of 50-99 μg/L], more than adequate iodine intake （MUI of 200-299 μg/L）, and excessive iodine intake （MUI over 300 μg/L）. Univariate and multivariate analysis （logistic regression analysis） were used to analyze the risk factors of subclinical hypothyroidism and subclinical hyperthyroidism. Logistic regression analysis with sex and age controlled suggested that more than adequate iodine intake （OR = 3.172, P = 0.0004） and excessive iodine intake （OR = 6.391, P = 0.0001） increased the risk of subclinical hypothyroidism, while excessive iodine intake decreased the risk of subclinical hyperthyroidism （OR = 0.218, P= 0.0001）. Logistic regression analysis including interaction of iodine intake and antibodies [tryroid peroxidase antibody （TPOAb） and thyroglobulin antibody （TgAb）] suggested that excessive iodine intake was an independent risk factor of subclinical hypothyroidism （OR = 6.360, P= 0.0001）, but independent protect factor of subclinical hyperthyroidism （OR = 0.193, P= 0.0001）. More than adequate iodine intake and it＇s interaction with TgAb increased the risk of subclinical hypothyroidism independently, in addition, it decreased the risk of subclinical hyperthyroidism at the present of TPOAb. Conclusion Both excessive iodine intake and more than adequate iodine intake could increase risk of subclinical hypothyroidism, supplement of iodine should be controlled to ensure MUI within the safe range.

Thermal behavior and mass spectrometry analysis of N-methyl-N-nitroso-p-toluenesulfonamide

The effect of temperature on the functional groups transition of N-methyl-N-nitroso-p-toluenesulfonamide （Diazald） and thermal decomposition were investigated by Fourier Transform Infrared Spectroscopy （FT-IR） and Differential Scanning Calorimeter （DSC）. The results showed that the functional groups transition of Diazald was temperature dependent, and thermal decomposition of Diazald was accelerated above 47.7℃. The HPLC-ESI-MS method was used for Diazald analysis, which indicated the strong hydrogen bonding between Diazald and water and instability of the NO group.