富甲烷生理盐水对大鼠急性脊髓损伤的治疗作用及剂量效应研究

目的研究富甲烷生理盐水(MS)腹腔注射对大鼠脊髓损伤的治疗作用及剂量效应关系。方法 50只雌性SD大鼠,随机取40只采用改良Allen法制作大鼠脊髓损伤模型,其余10只纳入假手术组。40只脊髓损伤大鼠随机分为脊髓损伤组、0.5 ml/kg MS组、5 ml/kg MS组、20 ml/kg MS组,每组10只。0.5 ml/kg MS组、5 ml/kg MS组、20 ml/kg MS组大鼠每12 h腹腔内注射相应剂量的MS。造模72 h后处死大鼠,迅速取出以脊髓损伤部位为中心、周围6 mm内组织,检测超氧化物歧化酶(SOD)、丙二醛(MDA)、TNF-α、IL-1β和IL-6含量。结果与假手术组比较,脊髓损伤组大鼠脊髓组织中MDA含量显著升高,而SOD活性显著降低,IL-1β、TNF-α、IL-6含量显著升高,差异有统计学意义(P <0.05)。与脊髓损伤组比较,0.5 ml/kg MS组、5 ml/kg MS组、20 ml/kg MS组MDA含量逐渐降低,SOD活性逐渐升高,IL-1β、TNF-α、IL-6含量逐渐降低,差异有统计学意义(P <0.05)。结论大鼠腹腔注射MS可以通过抑制脊髓受损节段的炎症反应、氧化应激,起到治疗脊髓损伤的作用,剂量与效应呈正相关。

Protective effects of methane-rich saline on mice with allergic asthma by inhibiting inflammatory response, oxidative stress and apoptosis

Background:Asthma is a common cause of breathing difficulty in children and adults,and is characterized by chronic airway inflammation that is poorly controlled by available treatments.This results in severe disability and applies a huge burden to the public health system.Methane has been demonstrated to function as a therapeutic agent in many diseases.The aim of the present study was to explore the effect of methane-rich saline(MRS)on the pathophysiology of a mouse model of asthma and its underlying mechanism.Methods:A murine model of ovalbumin(OVA)-induced allergic asthma was applied in this study.Mice were divided into three groups:a control group,an OVA group,and OVA-induced asthmatic mice treated with MRS as the third group.Lung resistance index(RI)and dynamic compliance(Cdyn)were measured to determine airway hyper-responsiveness(AHR).Haematoxylin and eosin(H&E)staining was performed and scored to show histopathological changes.Cell counts of bronchoalveolar lavage fluid(BALF)were recorded.Cytokines interleukin(IL)-4,IL-5,IL-13,tumor necrosis factorα(TNF-α),and C-X-C motif chemokine ligand 15(CXCL15)from BALF and serum were measured by enzyme-linked immunosorbent assay(ELISA).The oxidative stress indexes,including malondialdehyde(MDA),superoxide dismutase(SOD),catalase(CAT),glutathione(GSH),myeloperoxidase(MPO),and 8-hydroxydeoxyguanosine(8-OHdG),were determined using commercial kits.Apoptosis was evaluated by western blot,quantitative real-time polymerase chain reaction(qRT-PCR),and biochemical examination.Results:MRS administration reversed the OVA-induced AHR,attenuated the pathological inflammatory infiltration,and decreased the cytokines IL-4,IL-5,IL-13,TNF-α,and CXCL15 in serum and BALF.Moreover,following MRS administration,the oxidative stress was alleviated as indicated by decreased MDA,MPO,and 8-OHdG,and elevated SOD and GSH.In addition,MRS exhibited an anti-apoptotic effect in this model,protecting epithelial cells from damage.Conclusions:Methane improves pulmonary function and decreases infiltrative inflammatory cells in the allergic asthmatic mouse model.This may be associated with its anti-inflammatory,antioxidative,and anti-apoptotic properties.