肾上腺素在亚甲绿碳糊修饰电极上的电催化氧化及流动注射分析

将亚甲绿混合在碳糊中制成化学修饰电极对肾上腺素（Ｅｐ）的氧化具有良好的电催化作用，降低Ｅｐ氧化过电位４００ｍＶ。该电极用于流动注射分析（ＦＩＡ）能大大提高Ｅｐ的检测灵敏度。其线性范围为１．０×１０７～５．０×１０４ｍｏｌ／Ｌ；检测限为１．０×１０８ｍｏｌ／Ｌ（０．２５ｐｍｏｌ，Ｓ／Ｎ＝３）。经过６ｄ连续测定，响应值仅下降 １８％。

六胺银甲绿派洛宁复染法

使用两种或两种以上染色相结合的染色方法,用以辨认比较复杂的病变,已成为病理组织学一种趋势。本文报道一种复合染色法——六胺银甲绿派洛宁复染法,用以显示桥本氏甲状腺炎浆细胞在甲状腺滤泡内及滤泡间的分布。一、溶液的配制:1.0.5%过碘酸。2.0.2%氯化金。3.5%硫代硫酸钠。4.5%硼砂液。5.六胺银储备液:5%硝酸银5ml,3%六亚甲四胺100ml。

生物分子电催化的研究——Ⅳ.NADH在亚甲绿修饰石墨电极上的电催化氧化

本文以强烈吸附在石墨电极上的亚甲绿作为电子传递媒介体构成修饰电极.在-0.25V～+0.10V电位区间内,吸附态的亚甲绿表现出相当可逆的氧化还原行为,电极反应有一个电子和一个质子参加.在pH=7.0的磷酸盐缓冲溶液中,其式量电位 E °’ 为-0.14V,表观电子传递速率常数K_(app)为4.4s^(-1).亚甲绿修饰电极对还原型烟酰胺腺嘌呤二核苷酸(NADH)的电化学氧化具有明显的催化作用,可使NADH的氧化过电位降低500mV,它作为NADH的电化学安培检测器具有很高的灵敏度和良好的重现性.文中还用X光电子能谱(ESCA)、衰减全反射红外光谱(ATR)等现代分析技术对修饰电极进行了表征.

氯仿-脂肪抽出器提纯甲绿的改进法

甲绿又名甲基绿,它为细的绿色结晶,呈淡绿色粉末状并发金黄色光泽。溶于水呈蓝绿色,微溶于醇;不溶于醚、氯仿或戊醇。其化学分子式为C26H33N3Cl2。商品通常为其锌复盐C26H33N3Cl2·ZnCl2。

足癣、甲真菌病合并绿甲综合征1例

患者女,21岁,双足趾缝发白、脱屑伴趾甲毁损5年。趾缝、趾甲荧光真菌镜检可见竹节样菌丝。左足拇趾甲屑细菌培养可见铜绿假单胞菌;药敏试验提示对喹诺酮类、头孢类等多种抗生素敏感。皮肤镜检查左足拇趾甲板下可见大理石样浑浊区,白色纵行条纹,锯齿状边缘,尖峰朝向近端,伴黄绿色色素条纹。诊断:足癣、甲真菌病合并绿甲综合征。临床上皮肤浅部真菌感染常见,但足癣、甲真菌病伴发绿甲相关报道较少。

外用左氧氟沙星单疗治愈绿甲综合征一例

患者,男,63岁。双手拇指甲板变绿半年。细菌培养结果为铜绿假单胞菌,根据药敏试验给予患者左氧氟沙星外用,治疗3个月痊愈。

甲念珠菌病并发皮肤念珠菌感染及绿甲综合征一例

临床上甲念珠菌病很常见,但是同一念珠菌菌株又同时引起皮肤浅部念珠菌感染、而且并发绿甲,比较少见。我院门诊近期诊治1例近平滑念珠菌致甲念珠菌病和皮肤浅部念珠菌感染并发绿甲综合征的病例,现报道如下。

绿甲1例

绿甲（green nails）是一种获得性感染性疾病，多处于铜绿假单胞菌在甲下生长，继而产生绿色素进入甲板致使指（趾）甲甲扳呈绿色。本病临床罕见报道，我院门诊近期诊治1例，现报告如下。

甲真菌病并发绿甲一例

绿甲（green nails）是一种获得性感染性疾病，多见于铜绿假单胞菌感染，继而产生绿色素进入甲板，致使甲板呈绿色。本病临床报道较少，甲真菌病并发绿甲的病例，近10年来未见报道，我科门诊近期诊治1例，现报告如下。

虫螨腈、高效氟氯氰菊酯等防治柑橘灰象甲、小绿象甲药效试验

对虫螨腈与氯氟氰菊酯进行防治柑橘灰象甲、小粉绿象甲的药效试验。结果表明,12.5%虫螨腈+氯氟氰菊酯EC、12.5%虫螨腈+高效氟氯氰菊酯EC可以用来防治柑橘灰象甲和小绿象甲,推荐使用倍液数为2 000～2 500倍液。

广谱杀虫、杀螨剂——5%甲维盐绿阴

5％甲维盐绿阴是广谱性杀虫、杀螨剂，具有高效、低毒、无公害、无残留、易降解等特点，安全性好。

甲分离研究进展

甲分离（onycholysis）也被称为甲剥离,是指甲板从甲床自发性的分离,为最常见的甲病症状之一[1]。随着社会的不断发展,人民生活水平有了明显的提高,甲病越来越受到了人们的重视,因甲分离而来就诊的门诊患者有了明显的增多。甲分离发病机制目前尚不清楚,也缺少有效的治疗方法[2]。

绿塑青春别样娇——略记博白县经济能人冯学坤、冯学全兄弟

地处博白南大门的英桥镇,气候宜人土地肥沃,山岭重叠连绵不断。尤其是低山坡、火甲塘一带的山脉,更是逶迤多姿气势不凡。一座座郁郁葱葱的奇峰,构成了一条条奔腾飞跃的龙;一个个千姿百态的峻岭,恰似一只只造型典雅的凤凰。而一丛丛清葱翠绿的龙眼果树,就像龙身上的闪光绿甲,就似凤身上绿铮铮的羽毛,欲使龙腾飞、凤起舞……这就是博白经济能人冯学坤、冯学全兄弟俩办起来的桂圆发展公司英坤水果场所在地。当你看到这浩瀚的山岭、壮观的景象和迷人的风采时,必定会赏心悦目击掌称妙,真正领略了'龙飞凤舞'这词的深刻含义。

MG-P-MY显示乳腺肿瘤细胞凋亡的组合染色法

本文应用甲基绿、口派洛宁Ｇ和马体黄（ＭＧ－Ｐ－ＭＹ）组合染色法，对凋亡细胞的核酸进行了染色。结果表明，该染色法能更好地显示凋亡细胞的ＤＮＡ和ＲＮＡ成分，色彩鲜艳、对比清晰，鉴别可靠，具有一定的实用性。

Influence of copper ions and calcium ions on adsorption of CMC on chlorite

The effects of copper ions and calcium ions on the depression of chlorite using CMC（carboxymethyl cellulose） as a depressant were studied through flotation tests,adsorption measurements,ζ potential tests and co-precipitation experiments.The results show that the electrostatic repulsion between the CMC molecules and the chlorite surfaces hinders the approach of the CMC to the chlorite while the presence of copper ions and calcium ions enhances the adsorption density of CMC.The action mechanisms of these two types of ions are different.Calcium ions can not adsorb onto the mineral surfaces,but they can interact with the CMC molecules,thus reducing the charge of the CMC and enhancing adsorption density.Copper ions can adsorb onto the mineral surfaces,which facilitates the CMC adsorption through acid/base interaction.The enhanced adsorption density is also attributed to the decreased electrostatic repulsion between the CMC and mineral surfaces as copper ions reduce the surface charge of both the mineral surfaces and the CMC molecules.

蒙药泡囊草炮制样品及未炮制样品总生物碱的检测

目的：探讨泡囊草根炮制及未炮制样品总生物碱含量的检测。方法：以氢溴酸东莨菪碱为对照品,用酸性溴甲粉绿为染色剂,采用酶标仪检测吸光度并测定总生物碱含量。利用酶标仪进行了总生物碱的检测,因其试剂用量小、成本低、结果可靠、可高通量检测、方法简便。结果表明氢溴酸东莨菪碱在3.12～18.72ul/ml之间与吸光度形成良好的线性,拟合回归方程为Y=134.62X-8.1137,r=0.9995（n=6）;炮制及未炮制泡囊草总生物碱含量分别为0.46%和0.29%。这为蒙药材泡囊草的质量控制可提供依据,为泡囊草炮制工艺的规范化和质量的可控性提供了科学依据。

Study on Absorptive Capacity to Formaldehyde and Physiological and Biochemical Changes of Scindapsus aureus Based on the Regulation of LaCl_3

[Objective] This study was to analyze LaCl3 sprayed onto Scindapsus au- reus on Chlorophyll content and plasmamembrane permeability of leaves of Scindap- sus aureus, in order to determinate a suitable dosage of LaCl3. [Method] With Scindapsus aureus as the test subject, 110 households of 20 communities in the New District of Nantong were selected to investigate formaldehyde concentration in indoor air; and effect of LaCl3 on absorptive capacity to formaldehyde of Scindapsus aureus was studied through fumigating in laboratory. Simultaneously, its physiological and biochemical mechanism of formaldehyde resistance was studied. [Result] The re- sults showed that formaldehyde pollution of indoor air was serious in the New District of Nantong, and the degree of pollution was related to the time after decoration. The suitable concentration of LaCl3 for Scindapsus aureus was 20 mg/L. The ability to ab- sorb formaldehyde of Scindapsus aureus, which was calculated by per unit leaf area, was enhanced after spraying the suitable concentration of LaCl3 by 19.75%. When Scindapsus aureus was stressed by formaldehyde, chlorophyll content decreased by 39.87%, membrane permeability and cumulative MDA amount increased by 8.17% and 56.92%, respectively, and POD activity increased by 11.32%. However, by com- paring the group pre-sprayed with LaCl3 and the group not sprayed with LaCl3 under formaldehyde stress, chlorophyll content of Scindapsus aureus reduced lessly, mem- brane permeability and MDA both increased lessly, while POD activity increased more. [Conclusion] This study provides a new thinking direction for broadening of rare earth application and control measures of indoor air pollution.

A New Reaction for Kinetic Spetrophotometric Determination of Trace Ruthenium——Catalytic Oxidation of Methyl Green by Bromate

A sensitive catalytic spectrophotometric method for the determination of ruthenium （Ⅲ） has been developed, based on its catalytic effect on the oxidation reaction of methyl green with potassium bromate in acid solution medium at 100℃. The above reaction is followed spectrophotometrically by measuring the decrease in the absorbance at 625 nm for the catalytic reaction of methyl green. The calibration curve for the recommended reaction-rate method was linear in the concentration range over 0.00-0.80 μg/L and the detection limit of the method for Ru （III） is 0.006 μg/L. Almost no foreign ions interfered in the determination at less than 25-fold concentration of Ru （Ⅲ）. The method is highly sensitive, more selective and very stable, and has been successfully applied for the determination of trace ruthenium in some ores and metallurgy products.

Mechanism of Chrome-free Tanning with Tetra-hydroxymethyl Phosphonium Chloride

Tetra-hydroxymethyl phosphonium chloride （THPC） has been considered as an important chrome-free tanning agent. To understand the THPC tanning mechanism, the structure, charge distribution, activity and tanning ability of each phosphorous compound in THPC tanning system were studied, by ^31p NMR, FT-IR spectroscopy, differential scanning calorimetry （DSC） and computational chemistry method, etc. When pH raised to 6.0, the decomposition of THPC would take place, which results in a production of free formaldehyde, tri-hydroxymethyl phosphonium （TrHP） and tri-hydroxymethyl phosphine oxide （TrHPO）. At pH 9.0, THPC will be converted completely to TrHP and most TrHP is further oxidized into TrHPO. It is possible that, in reaction of phosphorous compounds and collagens, both P-C and C-O bonds would break simultaneously or individually. From molecular charge distribution and bond polar properties, it is deduced that, if P-C bonds break, the activity is in order of TrHPO 〉 THPC 〉 TrHE whereas if C--O bonds break, the order is TrHP 〉 THPC 〉 TrHPO. It is more possible that P--C bonds will break in reaction with collagen, and TrHPO may be more active in the THPC tanning system. The results of tanning and DSC also prove the above conclusion. Furthermore, the fact that the shrinkage temperature of THPC tanned leather was below 70℃ when basified to pH 5.0 or lower suggests that the hydroxymethyl groups of THPC and TrHP are less possible to combine directly with amino groups of collagen.