Objective. To detect histological characteristic of anterior cruciate ligament (ACL) and medial collateral ligament (MCL). Methods. In each of 20 skeletally mature male mongrels and 4 men, the ACL and MCL were examine...Objective. To detect histological characteristic of anterior cruciate ligament (ACL) and medial collateral ligament (MCL). Methods. In each of 20 skeletally mature male mongrels and 4 men, the ACL and MCL were examined by standard hematoxylin- eosin procedure and toluidine blue staining for histologic observation. Results. The fibroblasts in medial collateral are elongated to spindle shape and aligned in a row between the bundles of collagenous fibers. Toluidine blue staining is negative. The anterior cruciate ligament demonstrated more heterogenous cell types and arrangement. It had three major cell forms:spindle, round and ovoid type, which were shorter but greater than the cells in medial collateral ligament. Toluidine blue staining was positive in anterior cruciate ligament. Most cells in anterior cruciate ligament were enclosed within lacunae. Conclusion. This study suggests that the ACL has different histological characteristics from MCL, and is more cartilage- like in nature.展开更多
目的研究建立台盼蓝-伊红(trypan blue and eosin,TE)染色法,并用于强酸脱钙后的骨组织病理切片染色,以提高制片质量。方法对10只大鼠胫骨进行5%硝酸脱钙、石蜡包埋、连续切片后,对每份胫骨标本分别进行苏木素-伊红(hematoxylin and eos...目的研究建立台盼蓝-伊红(trypan blue and eosin,TE)染色法,并用于强酸脱钙后的骨组织病理切片染色,以提高制片质量。方法对10只大鼠胫骨进行5%硝酸脱钙、石蜡包埋、连续切片后,对每份胫骨标本分别进行苏木素-伊红(hematoxylin and eosin,HE)染色和甲苯胺蓝(toluidine blue,TB)染色及TE染色,比较不同染色方法的骨组织病理切片显微结构染色的差异。结果 10只大鼠胫骨组织标本每只分别进行3种染色,其中HE染色的骨小梁与骨髓分色不明显,骨细胞核无明显着色;TB染色的骨小梁与骨髓分色不明显,骨细胞边界模糊;TE染色的骨小梁显绛红色,骨髓细胞显紫红色,骨细胞陷窝、细胞核、骨小梁及附着的成骨细胞和破骨细胞、骨髓细胞中的巨噬细胞等结构特征清晰可辨。结论本研究所用的TE染色法适用于强酸脱钙处理的骨组织病理制片。展开更多
基金the Science Foundation of Ministry of Health, PRC (96- 1- 263)
文摘Objective. To detect histological characteristic of anterior cruciate ligament (ACL) and medial collateral ligament (MCL). Methods. In each of 20 skeletally mature male mongrels and 4 men, the ACL and MCL were examined by standard hematoxylin- eosin procedure and toluidine blue staining for histologic observation. Results. The fibroblasts in medial collateral are elongated to spindle shape and aligned in a row between the bundles of collagenous fibers. Toluidine blue staining is negative. The anterior cruciate ligament demonstrated more heterogenous cell types and arrangement. It had three major cell forms:spindle, round and ovoid type, which were shorter but greater than the cells in medial collateral ligament. Toluidine blue staining was positive in anterior cruciate ligament. Most cells in anterior cruciate ligament were enclosed within lacunae. Conclusion. This study suggests that the ACL has different histological characteristics from MCL, and is more cartilage- like in nature.
文摘目的研究建立台盼蓝-伊红(trypan blue and eosin,TE)染色法,并用于强酸脱钙后的骨组织病理切片染色,以提高制片质量。方法对10只大鼠胫骨进行5%硝酸脱钙、石蜡包埋、连续切片后,对每份胫骨标本分别进行苏木素-伊红(hematoxylin and eosin,HE)染色和甲苯胺蓝(toluidine blue,TB)染色及TE染色,比较不同染色方法的骨组织病理切片显微结构染色的差异。结果 10只大鼠胫骨组织标本每只分别进行3种染色,其中HE染色的骨小梁与骨髓分色不明显,骨细胞核无明显着色;TB染色的骨小梁与骨髓分色不明显,骨细胞边界模糊;TE染色的骨小梁显绛红色,骨髓细胞显紫红色,骨细胞陷窝、细胞核、骨小梁及附着的成骨细胞和破骨细胞、骨髓细胞中的巨噬细胞等结构特征清晰可辨。结论本研究所用的TE染色法适用于强酸脱钙处理的骨组织病理制片。