Background: Our previous study showed that large keratohyaline granules (KHG) in molluscum contagiosum that stained with haematoxylin also reacted with anti- Ted- H- 1 monoclonal antibody (mAb), but not with antifilag...Background: Our previous study showed that large keratohyaline granules (KHG) in molluscum contagiosum that stained with haematoxylin also reacted with anti- Ted- H- 1 monoclonal antibody (mAb), but not with antifilaggrin mAb or antiloricrin polyclonal antibody (pAb). This finding indicated that the Ted- H- 1 antigenic protein is a haematoxylin- stainable protein in KHG. Objectives: To clarify the identity of the major component protein of the large KHG in solar keratosis, another disorder in which large KHG are observed. Methods An enzyme immunohistochemical study was performed using antifilaggrin mAb, anti- Ted- H- 1 mAb and antiloricrin pAb. Immunofluorescent double staining and immunoelectron microscopic analyses were performed using anti- Ted- H- 1 mAb and antiloricrin pAb. Results: Antifilaggrin mAb, anti- Ted- H- 1 mAb and antiloricrin pAb reacted with normal KHG in nonlesional skin of solar keratosis, while only anti- Ted- H- 1 mAb reacted with the large KHG in the lesions of solar keratosis. Antifilaggrin mAb did not react with large KHG. Antiloricrin pAb reacted with the cell membrane of the stratum granulosum, but not with large KHG. Conclusions: These findings suggest that the haematoxylin- stainable protein in the large KHG would be a Ted- H- 1 antigen protein which was neither filaggrin nor loricrin.展开更多
文摘Background: Our previous study showed that large keratohyaline granules (KHG) in molluscum contagiosum that stained with haematoxylin also reacted with anti- Ted- H- 1 monoclonal antibody (mAb), but not with antifilaggrin mAb or antiloricrin polyclonal antibody (pAb). This finding indicated that the Ted- H- 1 antigenic protein is a haematoxylin- stainable protein in KHG. Objectives: To clarify the identity of the major component protein of the large KHG in solar keratosis, another disorder in which large KHG are observed. Methods An enzyme immunohistochemical study was performed using antifilaggrin mAb, anti- Ted- H- 1 mAb and antiloricrin pAb. Immunofluorescent double staining and immunoelectron microscopic analyses were performed using anti- Ted- H- 1 mAb and antiloricrin pAb. Results: Antifilaggrin mAb, anti- Ted- H- 1 mAb and antiloricrin pAb reacted with normal KHG in nonlesional skin of solar keratosis, while only anti- Ted- H- 1 mAb reacted with the large KHG in the lesions of solar keratosis. Antifilaggrin mAb did not react with large KHG. Antiloricrin pAb reacted with the cell membrane of the stratum granulosum, but not with large KHG. Conclusions: These findings suggest that the haematoxylin- stainable protein in the large KHG would be a Ted- H- 1 antigen protein which was neither filaggrin nor loricrin.
