甲醇老化处理对棉花种子发芽和幼苗生长的影响

以中棉所42为实验材料,用50%甲醇人工老化处理棉花种子0(ck),30,60min和90min,测定其发芽指标(发芽率、发芽势、发芽指数和活力指数)和生长指标(主根长度和幼苗鲜重)以及相对电导率。结果表明:随着老化程度的加深,发芽指标均呈现下降趋势,各指标在老化处理后与对照相比分别降低6.00%~19.34%、4.00%~24.66%、0.36%~4.49%和5.04%~14.06%。老化过程中,幼苗主根长度和鲜重随着老化时间的延长总体均降低,而相对电导率随老化时间的延长总体逐渐升高。

甲醇老化处理对玉米种子发芽和幼苗生长的影响

分析不同时间的50%甲醇老化处理对玉米种子发芽和幼苗生长的影响,明确甲醇老化处理的最佳时间。结果表明,与蒸馏水浸泡40 min（CK）相比,50%甲醇老化处理10-30 min时种子发芽指标（活力指数除外）均无显著变化;40 min时种子发芽率、发芽势、发芽指数、活力指数分别降低5.67%、8.67%、11.08%和41.22%;50 min时分别降低6.00%、10.00%、18.05%和47.70%;60 min时分别降低21.33%、28.00%、35.62%和61.80%;70 min时种子发芽指标降至较低水平。甲醇老化处理10 min时,玉米幼苗苗高、根长、根苗比、苗重和根重均显著降低,且降低幅度高于发芽指标。与CK相比,甲醇老化处理10-30 min时对种子脱氢酶活性和相对电导率无显著影响,40-70 min时脱氢酶活性显著降低,相对电导率显著增加。因此,50%甲醇老化处理应选择40-60 min为宜。

Preliminary study of therapeutic effects of panax notoginside and methylprednisolone on pulmonary fibrosis in rats

Objective: To study the therapeutic effects of panax notoginside (PN) and methylprednisolone (MP) on pulmonary fibrosis in rats. Methods: Bleomycin was introduced into the bronchial tree of 75 Wistar male rats through a tracheal incision to establish a rat model of pulmonary fibrosis (PF). The rats were equally divided into 3 groups: PF group, PN treated group and MP treated group. Five rats of each group were killed 1, 3, 7, 14 and 28 d after the administration of bleomycin and the specimens of lung tissue and plasma were collected for the determination of content of collagen Ⅰ and Ⅲ with immunohistochemical method and the level of MIP-1α and MCP-1 with ELIZA. Results: The severity of PF, the content of collagen Ⅰ and Ⅲ and the level of MIP-1α and MCP-1 were significantly decreased in PN and MP treated groups than in PF group (P<0.05). Conclusion: PN and MP are effective to control the development of PF induced with bleomycin in rats.