The effects of cultural conditions,which were fermentation period when methanol was as sole carbon source,methanol concentration,range of pH,on the expression of the chimeric protein of Plasmodium falciparum in geneti...The effects of cultural conditions,which were fermentation period when methanol was as sole carbon source,methanol concentration,range of pH,on the expression of the chimeric protein of Plasmodium falciparum in genetically engineered methylotrophic Pichia pastoris were investigated by shake flask experiments in this paper.The results showed:(1)fermentation period with methanol inducement is about 96 hours;(2)the optimum methanol concentration as sole carbon source is 10g/L;(3)the range of pH is from 6.0 to 7.0. On the base of above experimental results the cell high-density fermentation had been done on the FMG-5L fermentor with multi-sensors.The results showed that the cell optical density (OD 600) can reached 550 and the ma-ximum expression level of target proteins was 780mg/L which was 4 times higher or more than the shake flask culture’s.展开更多
Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been c...Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments.展开更多
文摘The effects of cultural conditions,which were fermentation period when methanol was as sole carbon source,methanol concentration,range of pH,on the expression of the chimeric protein of Plasmodium falciparum in genetically engineered methylotrophic Pichia pastoris were investigated by shake flask experiments in this paper.The results showed:(1)fermentation period with methanol inducement is about 96 hours;(2)the optimum methanol concentration as sole carbon source is 10g/L;(3)the range of pH is from 6.0 to 7.0. On the base of above experimental results the cell high-density fermentation had been done on the FMG-5L fermentor with multi-sensors.The results showed that the cell optical density (OD 600) can reached 550 and the ma-ximum expression level of target proteins was 780mg/L which was 4 times higher or more than the shake flask culture’s.
文摘Methylotrophic yeast has been used as a cost-effective and valuable host for expression of recombinant protein due to its unique methanol utilisation pathway. It has an AOX （alcohol oxidase） protein which has been characterised to be a strong and tightly methanol-inducible dependent promoter. Metabolomics is the systematic study and inclusive analysis of small molecules called metabolites in a biological system. Metabolomics plays an important part in connecting the phenotype and genotype gap because it magnifies the modifications in the proteome and provides a better phenotype representation of an organism. This quantitative study has provided a new perception on the metabolic burden derived from the overexpression of recombinant protein in methylotrophic yeast. In this review, we discuss the fundamental aspect of metabolomics in methylotrophic yeast followed by their latest developments.
