The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator....The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods： alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows： （a） no additive; （b） methyl paraben （0.3%）; （c） propyl paraben （0.3%）; （d） sodium metabisulfite （0.01%）; （e） A combination of methyl paraben （0.3%） and propyl paraben （0.3%）; （f） A combination of methyl paraben （0.3%）, propyl paraben （0.3%） and sodium metabisulfite （0.01%）. A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time （within the study period）. Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.展开更多
From 1592 to 1945, Japan conducted a series of military incursions in East Asia, including the invasion of Korea of 1592-1598, the Sino-Japanese War of 1894-1895 and the Japanese War of Aggression against China of the...From 1592 to 1945, Japan conducted a series of military incursions in East Asia, including the invasion of Korea of 1592-1598, the Sino-Japanese War of 1894-1895 and the Japanese War of Aggression against China of the 1930s-1940s. The tone of this series of aggressive actions was set by Toyotomi Hideyoshi, was continued in the Meiji Restoration and magnified in the Showa era, crystallizing into a pervading pattern of thought and action hiding behind the facts. Those post-World War II views that propose to cut the connections among these wars launched by Japan do not conform to the way Japanese said and behaved in the past but merely endorse some contemporary Japanese academic opinions. Therefore, exploring the facts and hidden logic linking these wars and restoring the original attributes of each regional war may be of benefit to fundamental studies and realistic concerns about Eastern Asian issues.展开更多
文摘The fresh leaves of Combretum micranthum were harvested during the rainy season in Akwa Ibom State, washed and subjected to extraction by maceration in distilled water. Concentration was done with a rotary evaporator. The extracts were tested for content of the following phytochemicals using standard methods： alkaloids, saponins, tannins, anthraquinones, cardiac glycosides, flavonoids and steroids. Antibacterial sensitivity test of the extract was carried out on S. aureus, B. subtilis and E. coli using agar well diffusion method and Ampiclox as control. The effect of storage time of the extract on its antibacterial activity was determined after the extract was kept at room temperature for 1 h, 24 h, 48 h and 72 h, respectively. Preparations of 200 mg/mL of the aqueous extract were made with additives as follows： （a） no additive; （b） methyl paraben （0.3%）; （c） propyl paraben （0.3%）; （d） sodium metabisulfite （0.01%）; （e） A combination of methyl paraben （0.3%） and propyl paraben （0.3%）; （f） A combination of methyl paraben （0.3%）, propyl paraben （0.3%） and sodium metabisulfite （0.01%）. A visual observation of the stabilized preparations was employed on daily basis for 30 days. Phytochemical analyses on the aqueous extract of the fresh leaves showed the absence of alkaloids, anthraquinones and flavonoids. Saponins, cardiac glycosides and steroids were present in moderate amounts and there was abundance of tannins. The extract had similar effect on both S. aureus and E. coli cultures but activity was very low with B. subtilis. When S. aureus activity increased with time of storage up to 48 hours, thereafter the activity started reducing. However, the activity of E. coli continued to increase with time （within the study period）. Thus, it appears reasonable to store the extract for at least 48 hours before the use for enhanced activity.
文摘From 1592 to 1945, Japan conducted a series of military incursions in East Asia, including the invasion of Korea of 1592-1598, the Sino-Japanese War of 1894-1895 and the Japanese War of Aggression against China of the 1930s-1940s. The tone of this series of aggressive actions was set by Toyotomi Hideyoshi, was continued in the Meiji Restoration and magnified in the Showa era, crystallizing into a pervading pattern of thought and action hiding behind the facts. Those post-World War II views that propose to cut the connections among these wars launched by Japan do not conform to the way Japanese said and behaved in the past but merely endorse some contemporary Japanese academic opinions. Therefore, exploring the facts and hidden logic linking these wars and restoring the original attributes of each regional war may be of benefit to fundamental studies and realistic concerns about Eastern Asian issues.
