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基于磁性纳米颗粒和金纳米粒子构建DNA电化学生物传感技术 被引量:3
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作者 王小兰 郑静 +3 位作者 陈琛 汤亚泥 张帆 何品刚 《分析科学学报》 CAS CSCD 北大核心 2014年第4期477-480,共4页
本文构建了一种基于纳米粒子、茎环DNA和丝网印刷电极(SPCE)的电化学生物传感技术用于乳腺癌基因的快速、灵敏检测。该传感技术中,探针DNA的两端分别标记了巯基和生物素,巯基用于与金纳米粒子(AuNPs)作用,生物素用于与磁性纳米颗粒(MNPs... 本文构建了一种基于纳米粒子、茎环DNA和丝网印刷电极(SPCE)的电化学生物传感技术用于乳腺癌基因的快速、灵敏检测。该传感技术中,探针DNA的两端分别标记了巯基和生物素,巯基用于与金纳米粒子(AuNPs)作用,生物素用于与磁性纳米颗粒(MNPs)表面修饰的链酶亲和素作用以达到富集的目的,之后利用SPCE进行电化学检测。无目标DNA存在时,双标记DNA保持茎环结构,使得生物素分子很难和MNPs上的亲和素接触。一旦加入目标DNA,茎环结构打开,生物素得以与MNPs上的链霉亲和素发生特异性结合,形成的复合物(MNPs-DNA-AuNPs)通过磁性富集到SPCE表面,从而获得AuNPs的电化学信号。该DNA电化学生物传感对单碱基错配有良好的分辨能力,完全互补DNA的检出限为8.0×10-13 mol/L。 展开更多
关键词 本文构建了一种基于纳米粒子、茎环DNA和丝网印刷电极(SPCE)的电化学生物技术用于乳腺癌基因的快速、灵敏检测.该技术中 探针DNA的两端分别标记了巯基和生物 巯基用于与金纳米粒子(AuNPs)作用 生物素用于与磁性纳米颗粒(MNPs)表面修饰的链酶亲和素作用以达到富集的目的 之后利用SPCE进行电化学检测.无目标DNA存在时 双标记DNA保持茎环结构 使得生物素分子很难和MNPs上的亲和素接触.一旦加入目标DNA 茎环结构打开 生物素得以与MNPs上的链霉亲和素发生特异性结合 形成的复合物(MNPs-DNA-AuNPs)通过磁性富集到SPCE表面 从而获得AuNPs的电化学信号.该DNA电化学生物对单碱基错配有良好的分辨能力 完全互补DNA的检出限为8 0×10-13 mol L.本文构建了一种基于纳米粒子、茎环DNA和丝网印刷电极(SPCE)的电化学生物技术用于乳腺癌基因的快速、灵敏检测.该技术中 探针DNA的两端分别标记了巯基和生物 巯基用于与金纳米粒子(AuNPs)作用 生物素用于与磁性纳米颗粒(MNPs)表面修饰的链酶亲和素作用以达到富集的目的 之后利用SPCE进行电化学检测.无目标DNA存在时 双标记DNA保持茎环结构 使得生物素分子很难和MNPs上的亲和素接触.一旦加入目标DNA 茎环结构打开 生物素得以与MNPs上的链霉亲和素发生特异性结合 形成的复合物(MNPs-DNA-AuNPs)通过磁性富集到SPCE表面 从而获得AuNPs的电化学信号.该DNA电化学生物对单碱基错配有良好的分辨能力 完全互补DNA的检出限为8 0×10-13 mol L.磁性纳米颗粒 金纳米粒子 DNA 丝网印刷电极
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电化学生物传感在HIV检测中的应用进展
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作者 邓培雪 叶力 +3 位作者 陈荣凤 安三奇 蒋俊俊 梁浩 《中国公共卫生》 CAS CSCD 北大核心 2023年第7期924-929,共6页
电化学生物传感器具有小型化、低成本、灵敏度高、检测快速、检测限低等优点,在HIV病毒检测领域受到广泛关注。本文综述了电化学生物传感技术用于HIV核酸或抗原检测的研究进展,并与传统检测方法进行对比,分析电化学生物传感检测的优劣势。
关键词 HIV 电化学生物传感检测 核酸 HIVp24 GP120
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A Novel 3-D Nano-assembly Bacteria Based Biosensor for Enhanced Detection of Heavy Metal Pollutants
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作者 Mina Souiri Ibtissem Gammoudi +6 位作者 Laurence Mora Hafedh Ben Ouada Thierry Jouenne Nicole Jaffrezic-Renault Corinne Dejous Ali Othmane Anthony C. Duncan 《Journal of Environmental Science and Engineering(A)》 2012年第7期924-935,共12页
Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficul... Nowadays, at a time of growing concern for sustainable development and compliance with environmental standards and legislation, the detection of heavy metal contaminants in environmental matrices represents a difficult but important task. The current major limitation lies in the poor detection limits of the targeted pollutant's trace concentrations by the available conventional techniques. In order to elaborate a novel "living" self assembled electrochemical 3-D biosensor, the authors propose a new concept to overcome this shortcoming. The advantages of the properties of polyelectrolyte-functionalized NBs (nanobeads) are combined along with the use of non covalently strongly bound micro-organisms. The designed 3-D biosensor is all the more promising as it has showed a significantly improved sensitivity. In fact, the detection limits of the tested heavy metals (cadmium and mercury) were as low as 1.0 × 10^-12 mol.L-1 and six to seven orders of magnitude lower than those provided by conventional 2-D biosensors. Furthermore, it is potentially applicable to a wide range of bioreceptor-pollutant detection systems. 展开更多
关键词 BIOSENSOR nanobeads PEM heavy metals E. coli EIS (electrochemical impedance spectroscopy).
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Bimetallic mixed-linker metal-organic frameworks for electrochemical detection of biomolecules
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作者 YU Aixuan HAO Cundi +5 位作者 YANG Chunlei LIU Qing LI Jiajia BO Xiangjie DU Dongying SU Zhongmin 《分子科学学报》 CAS 2024年第2期138-161,共24页
Because of their easy tunability in structure,porosity,and micro-environment,metal-organic frameworks(MOFs)have recently attracted numerous attentions in various fields.The detection of ascorbic acid(AA),dopamine(DA),... Because of their easy tunability in structure,porosity,and micro-environment,metal-organic frameworks(MOFs)have recently attracted numerous attentions in various fields.The detection of ascorbic acid(AA),dopamine(DA),and uric acid(UA)is of great significance not only in biomedicine and neurochemistry but also in disease diagnosis and pathology research.Herein,a series of bimetallic-organic frameworks,MIL-125(Ti-Fe)-x%NH_(2)(x=0,25,50,75,and 100),was successfully synthesized.MIL-125(Ti-Fe)-x%NH_(2)family was employed as electrochemical sensors for the detection of AA,DA,and UA,and MIL-125(Ti-Fe)-100%NH_(2)exhibited the most promising performance with 50%carbon black doping in 0.1 mol·L^(-1)PBS(pH=7.10).In addition,the as-prepared MIL-125(Ti-Fe)-100%NH_(2)/GCE exhibited excellent anti-interference performance and good stability,which provided a promising platform for future utilization in real sample analysis. 展开更多
关键词 metal-organic frameworks biomolecule detection electrochemical senso
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Metal chelate affinity to immobilize horseradish peroxidase on functionalized agarose/CNTs composites for the detection of catechol 被引量:1
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作者 TU XinMan LUO ShengLian +3 位作者 LUO XuBiao ZHAO YingJie FENG Li LI JingHong 《Science China Chemistry》 SCIE EI CAS 2011年第8期1319-1326,共8页
The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes comp... The paper reports a novel amperometric biosensor for catechol based on immobilization of a highly sensitive horseradish peroxidase by affinity interactions on metal chelate-functionalized agarose/carbon nanotubes composites. Metal chelate affinity takes advantage of the affinity of Ni2+ ions to bind strongly and reversibly to histidine or cysteine tails found on the surface of the horseradish peroxidase. Thus, enzymes with such residues in their molecules can be easily attached to functionalized aga- rose/carbon nanotubes composites support containing a nickel chelate. Linear sweep voltammograms and amperometry are used to study the proposed electrochemical biosensor. Catechol is determined by direct reduction of biocatalytically liberated quinone species at -0.05 V (vs. SCE). The effect ofpH, applied electrode potential and the concentration of H2O2 on the sensitivity of the biosensor has been investigated. The performance of the proposed biosensor is tested using four different phenolic compounds, showing very high sensitivity, in particular, the linearity of cateehol is observed from 2.0 × 10-8 to 1.05×10-5 M with a detection limit of 5.0×10-9 M. 展开更多
关键词 amperometric biosensor carbon nanotubes metal chelation AGAROSE CATECHOL
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