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蚕蛹中蛋白质与甲壳分离的研究 被引量:6
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作者 陈玲 魏文静 +3 位作者 祝东怡 吴飞燕 郭玉琼 郑仕远 《化学工程师》 CAS 2007年第6期10-13,共4页
以脱脂蛹为原料,采用电子光学显微分析技术,观察酸、碱浸泡样组织结构变化,确定了碱法为蛋白质与甲壳素的分离方法。选定了NaOH溶液为分离试剂,就工艺条件(温度、碱浓度、固液比、时间)对蚕蛹蛋白与甲壳素分离效果的影响进行了研究。结... 以脱脂蛹为原料,采用电子光学显微分析技术,观察酸、碱浸泡样组织结构变化,确定了碱法为蛋白质与甲壳素的分离方法。选定了NaOH溶液为分离试剂,就工艺条件(温度、碱浓度、固液比、时间)对蚕蛹蛋白与甲壳素分离效果的影响进行了研究。结果表明,在本实验条件下,最佳工艺条件:温度80℃,固液比1:12,时间10min,碱浓度1(wt)%。甲壳得率为2.46%,粗蛋白回收得率为78.91%。 展开更多
关键词 电子光学显微分析 蚕蛹 蛋白 甲壳 分离
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Applications of scanning electron microscopy in earth sciences 被引量:7
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作者 CHEN Li XU Jun CHEN Jing 《Science China Earth Sciences》 SCIE EI CAS CSCD 2015年第10期1768-1778,共11页
This paper expounds upon the basic principle of scanning electron microscopy(SEM),the main features of image types,and different signals,and the applications and prospects in earth sciences research are reviewed.High-... This paper expounds upon the basic principle of scanning electron microscopy(SEM),the main features of image types,and different signals,and the applications and prospects in earth sciences research are reviewed.High-resolution field emission SEM allows observation and investigation of a very fine micro area in situ.Using low-vacuum mode SEM,geological insulating samples can be analyzed directly without coating,demonstrating the wide application prospect.Combined with backscatter detector(BSE),energy dispersal X-ray spectroscopy(EDS),cathodoluminescence spectrometry(CL),and electron back-scattering diffraction(EBSD),SEM can yield multiple types of information about geological samples at the same time,such as superficial microstructure,CL analysis,BSE image,component analysis,and crystal structure features.In this paper,we use examples to discuss the geological application of SEM.We stress that we should not only focus on the CL image analysis,but strengthen CL spectrum analyses of minerals.These results will effectively reveal the mineral crystal lattice defects and trace element composition and can help to reconstruct mineral growth conditions precisely. 展开更多
关键词 scanning electron microscopy signal detector MINERALS
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