[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by...[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by reverse transcription-polymerase chain reaction（RT-PCR）.Then RT-PCR products were cloned into the PMD18-T vector and sequenced.The functions of the sequence were predicted with bioinformatics method.[Result] A 1 079 bp gene was obtained.The gene encoded a protein with 236 amino acids.The protein contains many motif sites,two WRKY domains and a C2H2 zinc finger motif.The gene showed high identities with WRKY8,WRKY24 and WRKY30 gene of rice.[Conclusion] The up-regulated expression gene induced by R.solani was representative WRKY family gene.The gene could play an important role on rice sheath blight resistance.展开更多
BACKGROUND:The neurodevelopmental risks associated with high total serum bilirubin levels in newborns are not well defined. METHODS:We identified 140 infants with neonatal total serum bilirubin levels of at least 25mg...BACKGROUND:The neurodevelopmental risks associated with high total serum bilirubin levels in newborns are not well defined. METHODS:We identified 140 infants with neonatal total serum bilirubin levels of at least 25mg per deciliter (428 μmol per liter) and 419 randomly selected controls from a cohort of 106,627 term and near-term infants born from 1995 through 1998 in Kaiser Permanente hospitals in northern California. Data on outcomes were obtained from electronic records,interviews,responses to questionnaires,and neurodevelopmental evaluations that had been performed in a blinded fashion.RESULTS:Peak bilirubin levels were between 25 and 29.9 mg per deciliter (511 μmol per liter) in 130 of the newborns with hyperbilirubinemia and 30 mg per deciliter(513 μmol per liter) or more in 10 newborns; treatment involved phototherapy in 136 cases and exchange transfusion in 5. Follow-up data to the age of at least two years were available for 132 of 140 children with a history of hyperbilirubinemia (94 percent) and 372 of 419 controls (89 percent) and included formal evaluation at a mean (±SD) age of 5.1±0.12 years for 82 children (59 percent)and 168 children (40 percent),respectively. There were no cases of kernicterus. Neither crude nor adjusted scores on cognitive tests differed significantly between the two groups; on most tests,95 percent confidence intervals excluded a 3-point (0.2 SD) decrease in adjusted scores in the hyperbilirubinemia group. There was no significant difference between groups in the proportion of children with abnormal neurologic findings on physical examination or with documented diagnoses of neurologic abnormalities. Fourteen of the children with hyperbilirubinemia(17 percent) had “questionable”or abnormal findings on neurologic examination,as compared with 48 controls (29 percent; P = 0.05; adjusted odds ratio,0.47; 95 percent confidence interval,0.23 to 0.98; P =0.04). The frequencies of parental concern and reported behavioral problems also were not significantly different between the two groups. Within the hyperbilirubinemia group,those with positive direct antiglobulin tests had lower scores on cognitive testing but not more neurologic or behavioral problems. CONCLUSIONS:When treated with phototherapy or exchange transfusion,total serum bilirubin levels in the range included in this study were not associated with adverse neurodevelopmental outcomes in infants born at or near term.展开更多
The physical and mechanical change processes of coal and rock are closely related to energy transformation,and the destruction and failure of coal and rock is an instability phenomena driven by energy change.However,t...The physical and mechanical change processes of coal and rock are closely related to energy transformation,and the destruction and failure of coal and rock is an instability phenomena driven by energy change.However,the energy change of large-scale coal rock in the mine site is hardly calculated accurately,making it difficult to monitor coal-rock systematic failure and collapse from the perspective of energy.By the energy dissipation EMR monitoring system,we studied the damage and failure of coal and rock with bursting liability from the energy dissipation point using the geophysical method-EMR,and explored the energy dissipation characteristics during uniaxial compression and their main influencing factors.The results show that under displacement-control loading mode,there are 2 types of energy dissipation trends for both coal and rock with bursting liability.The type Ⅰ trend is a steady increase one during the whole process,therein,the energy dissipation of rock samples is accelerated at the peak load.The type Ⅱ trend energy is a W-shaped fluctuating one containing 6 stages.Under load-control loading mode,there is one energy dissipation trend of shock downward-steady rise.Besides that,rock samples also present a trend of 4 stages.The energy dissipation characteristics of coal and rockduring loading failure process can be used as effective criteria to assess whether they are in a stable or destructive stage.The factors influencing energy dissipation in the loading failure process of coal and rock mainly include strength,homogeneity,and energy input efficiency.展开更多
Rock bursts are serious natural disasters encountered worldwide in coal mining and rock engineering.In order to forecast rock bursts more effectively,a new rock burst forecasting index E,consisting of intensity and th...Rock bursts are serious natural disasters encountered worldwide in coal mining and rock engineering.In order to forecast rock bursts more effectively,a new rock burst forecasting index E,consisting of intensity and the number of pulses,is proposed,on the basis of abnormal characteristic symptoms of electromagnetic radiation(EMR) generated before rock bursts,combined with statistical theory.The index is distributed as a χ2 distribution with 2 degrees of freedom,i.e.,E～χ 2(2).Via this index,a quantitative comprehensive forecasting criterion of EMR was initially established.E values were calculated when the occurrence probability of the occurrence of a rock burst was 50%,70% and 90%.Appropriate measures should be taken when using these values on the scene.Using EMR data collected in the Nanshan Mine of the Hegang mining area,we verified that the analytical result were consistent with actual situations.This index is of theoretical importance and as a reference for forecasting rock bursts in coal mines.展开更多
The present study analyzed the electromagnetic radiation(EMR) time series of the destruction process of coal or rock sample under uniaxial loading and the monitoring process in working face by means of fractal geometr...The present study analyzed the electromagnetic radiation(EMR) time series of the destruction process of coal or rock sample under uniaxial loading and the monitoring process in working face by means of fractal geometry,and results of the correlation dimension change curve of EMR time series were obtained.In the meantime,the current study also sought the fractal characteristic to the EMR signals by contrast to the change curve of EMR signals and explored the precursory phenomenon of rock burst.This paper concluded the main findings as followed:the EMR time series of the destruction process of coal or rock sample under uniaxial loading and the monitoring process in working face corresponded to fractal;the correlation dimension of EMR time series reflected the process of coal or rock damage deformation,that is,the inner damage of coal or rock made a change from random to order.In the field application,the correlation dimension served as a new index of forecasting the coal or rock dynamic disaster.展开更多
In order to improve patient care in the United States there,the government made a mandate called HIE(Health Information Exchange).This order was created from the belief that sharing digital health in-formation between...In order to improve patient care in the United States there,the government made a mandate called HIE(Health Information Exchange).This order was created from the belief that sharing digital health in-formation between,across,and within health communities will improve one's healthcare experience across their lifespan.Patient health information,i.e.the personal health record,should be shareable between healthcare providers;such as private practice physicians,home health agencies,hospitals and nursing care facilities.Most of the U.S.hospitals now have electronic health records,however,with a lack of standards for structuring health information and unified communication protocols to share health information across providers,only a small percentage of U.S.hospitals engage in computerized HIE.In order to understand barriers and facilitators in the U.S.of HIE adoption,we reviewed the published research literature between 2010 and 2015.Our search yielded 664 articles from Medline,PsychInfo,Global health,InSpec,Scopus and Business Source Complete databases.Thirty-nine articles met our inclusion criteria.This article presents the compiled organizational and end user barriers and facilitators along with suggested methods to achieve continuity of care through HIE.展开更多
基金Supported by Young Academic Backbone Support Program of Heilongjiang Province(1152G022)~~
文摘[Objective] The aim was to clone the up-regulated expression gene of rice induced by Rhizoctonia solani.[Method] The EST fragment K16 obtained by suppression subtraction hybridization（SSH）was cloned and confirmed by reverse transcription-polymerase chain reaction（RT-PCR）.Then RT-PCR products were cloned into the PMD18-T vector and sequenced.The functions of the sequence were predicted with bioinformatics method.[Result] A 1 079 bp gene was obtained.The gene encoded a protein with 236 amino acids.The protein contains many motif sites,two WRKY domains and a C2H2 zinc finger motif.The gene showed high identities with WRKY8,WRKY24 and WRKY30 gene of rice.[Conclusion] The up-regulated expression gene induced by R.solani was representative WRKY family gene.The gene could play an important role on rice sheath blight resistance.
文摘BACKGROUND:The neurodevelopmental risks associated with high total serum bilirubin levels in newborns are not well defined. METHODS:We identified 140 infants with neonatal total serum bilirubin levels of at least 25mg per deciliter (428 μmol per liter) and 419 randomly selected controls from a cohort of 106,627 term and near-term infants born from 1995 through 1998 in Kaiser Permanente hospitals in northern California. Data on outcomes were obtained from electronic records,interviews,responses to questionnaires,and neurodevelopmental evaluations that had been performed in a blinded fashion.RESULTS:Peak bilirubin levels were between 25 and 29.9 mg per deciliter (511 μmol per liter) in 130 of the newborns with hyperbilirubinemia and 30 mg per deciliter(513 μmol per liter) or more in 10 newborns; treatment involved phototherapy in 136 cases and exchange transfusion in 5. Follow-up data to the age of at least two years were available for 132 of 140 children with a history of hyperbilirubinemia (94 percent) and 372 of 419 controls (89 percent) and included formal evaluation at a mean (±SD) age of 5.1±0.12 years for 82 children (59 percent)and 168 children (40 percent),respectively. There were no cases of kernicterus. Neither crude nor adjusted scores on cognitive tests differed significantly between the two groups; on most tests,95 percent confidence intervals excluded a 3-point (0.2 SD) decrease in adjusted scores in the hyperbilirubinemia group. There was no significant difference between groups in the proportion of children with abnormal neurologic findings on physical examination or with documented diagnoses of neurologic abnormalities. Fourteen of the children with hyperbilirubinemia(17 percent) had “questionable”or abnormal findings on neurologic examination,as compared with 48 controls (29 percent; P = 0.05; adjusted odds ratio,0.47; 95 percent confidence interval,0.23 to 0.98; P =0.04). The frequencies of parental concern and reported behavioral problems also were not significantly different between the two groups. Within the hyperbilirubinemia group,those with positive direct antiglobulin tests had lower scores on cognitive testing but not more neurologic or behavioral problems. CONCLUSIONS:When treated with phototherapy or exchange transfusion,total serum bilirubin levels in the range included in this study were not associated with adverse neurodevelopmental outcomes in infants born at or near term.
基金supported by Youth Science Foundation of the National Natural Science Foundation of China(No.51104156)the Fundamental Research Funds for the Central Universities of China(No.2013QNB02)the 12th Five Year National Science and Technology Support Key Project of China(Nos. 2012BAK04B07-2 and 2012BAK09B01-04)
文摘The physical and mechanical change processes of coal and rock are closely related to energy transformation,and the destruction and failure of coal and rock is an instability phenomena driven by energy change.However,the energy change of large-scale coal rock in the mine site is hardly calculated accurately,making it difficult to monitor coal-rock systematic failure and collapse from the perspective of energy.By the energy dissipation EMR monitoring system,we studied the damage and failure of coal and rock with bursting liability from the energy dissipation point using the geophysical method-EMR,and explored the energy dissipation characteristics during uniaxial compression and their main influencing factors.The results show that under displacement-control loading mode,there are 2 types of energy dissipation trends for both coal and rock with bursting liability.The type Ⅰ trend is a steady increase one during the whole process,therein,the energy dissipation of rock samples is accelerated at the peak load.The type Ⅱ trend energy is a W-shaped fluctuating one containing 6 stages.Under load-control loading mode,there is one energy dissipation trend of shock downward-steady rise.Besides that,rock samples also present a trend of 4 stages.The energy dissipation characteristics of coal and rockduring loading failure process can be used as effective criteria to assess whether they are in a stable or destructive stage.The factors influencing energy dissipation in the loading failure process of coal and rock mainly include strength,homogeneity,and energy input efficiency.
基金supported by the National High Technology Research and Development Program of China (No.2006AA06Z119)the Ministry of Education Support Program for New Century Excellent Talent (No.NCET-06-0477)
文摘Rock bursts are serious natural disasters encountered worldwide in coal mining and rock engineering.In order to forecast rock bursts more effectively,a new rock burst forecasting index E,consisting of intensity and the number of pulses,is proposed,on the basis of abnormal characteristic symptoms of electromagnetic radiation(EMR) generated before rock bursts,combined with statistical theory.The index is distributed as a χ2 distribution with 2 degrees of freedom,i.e.,E～χ 2(2).Via this index,a quantitative comprehensive forecasting criterion of EMR was initially established.E values were calculated when the occurrence probability of the occurrence of a rock burst was 50%,70% and 90%.Appropriate measures should be taken when using these values on the scene.Using EMR data collected in the Nanshan Mine of the Hegang mining area,we verified that the analytical result were consistent with actual situations.This index is of theoretical importance and as a reference for forecasting rock bursts in coal mines.
基金supported by the Fundamental Research Funds for the Central Universities in China University of Mining and Technology (No. 2010QNB23)the Open Fund of Laboratory in China University of Mining and Technology (No. 2010-II-004)
文摘The present study analyzed the electromagnetic radiation(EMR) time series of the destruction process of coal or rock sample under uniaxial loading and the monitoring process in working face by means of fractal geometry,and results of the correlation dimension change curve of EMR time series were obtained.In the meantime,the current study also sought the fractal characteristic to the EMR signals by contrast to the change curve of EMR signals and explored the precursory phenomenon of rock burst.This paper concluded the main findings as followed:the EMR time series of the destruction process of coal or rock sample under uniaxial loading and the monitoring process in working face corresponded to fractal;the correlation dimension of EMR time series reflected the process of coal or rock damage deformation,that is,the inner damage of coal or rock made a change from random to order.In the field application,the correlation dimension served as a new index of forecasting the coal or rock dynamic disaster.
文摘In order to improve patient care in the United States there,the government made a mandate called HIE(Health Information Exchange).This order was created from the belief that sharing digital health in-formation between,across,and within health communities will improve one's healthcare experience across their lifespan.Patient health information,i.e.the personal health record,should be shareable between healthcare providers;such as private practice physicians,home health agencies,hospitals and nursing care facilities.Most of the U.S.hospitals now have electronic health records,however,with a lack of standards for structuring health information and unified communication protocols to share health information across providers,only a small percentage of U.S.hospitals engage in computerized HIE.In order to understand barriers and facilitators in the U.S.of HIE adoption,we reviewed the published research literature between 2010 and 2015.Our search yielded 664 articles from Medline,PsychInfo,Global health,InSpec,Scopus and Business Source Complete databases.Thirty-nine articles met our inclusion criteria.This article presents the compiled organizational and end user barriers and facilitators along with suggested methods to achieve continuity of care through HIE.
