To detect gatifloxacin(GAT)residue in swine urine,an electrochemical immunoassay was established.An indirect competitive immunoassay was developed,in which the coating antigen is immobilized in an enzyme-linked immuno...To detect gatifloxacin(GAT)residue in swine urine,an electrochemical immunoassay was established.An indirect competitive immunoassay was developed,in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay(ELISA)plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody.Horseradish peroxidase(HRP)conjugated to goat anti-rabbit IgG was used as the enzymatic label.A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator.The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration(IC50)of 8.9 ng/ml was obtained.Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin(3.0%),ciprofloxacin(3.0%),and ofloxacin(1.9%)among commonly used(fluoro)quinolones.In conclusion,the immunoassay system developed in this research can be used as a rapid,powerful and on-site analytical tool to detect GAT residue in foods and food products.展开更多
In this study,a natural cotton thread immunoassay device combined with gold nanorod(GNR) reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung canc...In this study,a natural cotton thread immunoassay device combined with gold nanorod(GNR) reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle(GNP) report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry(ASV) testing,which meet the requirement for clinical diagnosis.展开更多
基金supported by the National High-Tech R&D Program(863)of China(Nos.07AA10Z435 and 2007AA06A407)the National Natural Science Foundation of China(No.20675048)+1 种基金the Fundamental Research Funds for the Central Universities(No.65011121)the Shandong Provincial Natural Science Foundation(No.Y2008B31),China
文摘To detect gatifloxacin(GAT)residue in swine urine,an electrochemical immunoassay was established.An indirect competitive immunoassay was developed,in which the coating antigen is immobilized in an enzyme-linked immunosorbent assay(ELISA)plate and GAT residue from the sample competes with the limited binding sites in added anti-GAT antibody.Horseradish peroxidase(HRP)conjugated to goat anti-rabbit IgG was used as the enzymatic label.A carbon fiber working electrode was constructed and current signals were detected by using hydrogen peroxide as a substrate and hydroquinone as an electrochemical mediator.The electrochemical immunoassay was evaluated by analysis of GAT in buffer or swine urine and an average value of half inhibition concentration(IC50)of 8.9 ng/ml was obtained.Excellent specificity of the antibody was achieved with little cross-reaction with lomefloxacin(3.0%),ciprofloxacin(3.0%),and ofloxacin(1.9%)among commonly used(fluoro)quinolones.In conclusion,the immunoassay system developed in this research can be used as a rapid,powerful and on-site analytical tool to detect GAT residue in foods and food products.
基金financially supported by the National Natural Science Foundation of China (No. 21205094)NFFTBS (Nos. J1103311,J1210057)the New Faculty Startup Funds of Northwest University in Shaanxi Province (No. PR12011)
文摘In this study,a natural cotton thread immunoassay device combined with gold nanorod(GNR) reporter probe is developed for the rapid,sensitive and quantitative electrochemical determination of human ferritin,a lung cancer related biomarker.Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device.An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle(GNP) report probe.The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry(ASV) testing,which meet the requirement for clinical diagnosis.
