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小麦幼穗蛋白质双向电泳条件的优化 被引量:9
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作者 于松 高庆荣 +2 位作者 袁凯 张保雷 李楠楠 《基因组学与应用生物学》 CAS CSCD 北大核心 2012年第1期84-89,共6页
本研究以温光敏小麦为试材,用TCA/丙酮和酚提取法提取小麦幼穗蛋白样品,进行了双向电泳优化分析,并对双向电泳过程中出现的问题进行了讨论。结果表明,用TCA/丙酮法提取小麦幼穗蛋白质其产率(浓度)高于酚提取法。SDS-PAGE电泳显示,用TCA... 本研究以温光敏小麦为试材,用TCA/丙酮和酚提取法提取小麦幼穗蛋白样品,进行了双向电泳优化分析,并对双向电泳过程中出现的问题进行了讨论。结果表明,用TCA/丙酮法提取小麦幼穗蛋白质其产率(浓度)高于酚提取法。SDS-PAGE电泳显示,用TCA/丙酮提取法提取的蛋白质能获得较清晰条带,分辨率较高,而酚提取法提取的蛋白质其条带模糊,分辨率低。对蛋白质纯化除盐可以提高分辨率,减少横竖纹,获得背景清晰的圆形蛋白点。通过ImageMasterTM 2D Platinum5.0软件分析凝胶图谱,结果显示纯化后可降低噪点,纯化后蛋白点数可从未纯化蛋白点数的216增加到583。显然,采用TCA/丙酮法可获得高浓度高质量的蛋白质,而进一步纯化、除盐离子可进一步获得背景清晰可高重复性的电泳图谱。在双向电泳实验过程中,观察到一些异常缺陷胶的出现,如双向电泳图谱中蛋白点扩散,蛋白聚集形成斑点串,没有点或点很少,出现纵纹横纹及图谱扭曲等影响图谱质量的严重问题,本研究对这些问题做了分析并提出了解决方案。 展开更多
关键词 温光敏小麦 小麦蛋白质 TCA/丙酮提取法 酚提取法提取法 双向凝胶电泳 电泳条件优化
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Optimizing electrophoretic deposition conditions for enhancement in electrical conductivity of carbon fiber/carbon nanotube/epoxy hybrid composites 被引量:1
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作者 王一奇 BYUN Joon-hyung +1 位作者 KIM Byung-sun SONG Jung-il 《Journal of Central South University》 SCIE EI CAS 2012年第11期3017-3022,共6页
The effectiveness of optimizing electrical conductivity of carbon fiber/carbon nanotube (CNT)/epoxy hybrid composites via Taguchi method was demonstrated. CNTs were induced on carbon fabric by electrophoretic deposi... The effectiveness of optimizing electrical conductivity of carbon fiber/carbon nanotube (CNT)/epoxy hybrid composites via Taguchi method was demonstrated. CNTs were induced on carbon fabric by electrophoretic deposition (EPD) technique. The essential deposition parameters were identified as l) the deposition time, 2) the deposition voltage, 3) the mass fraction of CNTs in suspension, and 4) the distance between the electrodes. An experimental design was then performed to establish the appropriate levels for each factor. An orthogonal array of L9 (34) was designed to conduct the experiments. Electrical conductivity results were collected as the response. The relative influences of design parameters on the response were discussed. Using the model, signal to noise (S/N) ratio and response characteristics for the optimized deposition parameter combination were predicted. The results show clearly that the optimum condition of electrophoretic deposition (EPD) process improves the electrical conductivity of carbon/epoxy hybrid composites. 展开更多
关键词 design of experiment electrophoretic deposition carbon nanotube Taguchi method signal to noise (S/N) ratio analysisof variance (ANOVA)
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