A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,...A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,BMIMBF4). The conditions for the CE separation,ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions,the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME,thebaine,CP,and AC were 2.1×10-8,1.4×10-7,6.3×10-8,and 3.6×10-8 mol/L(S/N=3),respectively. The limits of quantitation(LOQ,S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME,3.6 ×10-6 mol/L for thebaine,6.5×10-7 mol/L for CP,and 4.6×10-7 mol/L for AC,respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.展开更多
文摘A new approach for the determination of methylephedrine hydrochloride(ME),thebaine,codeine phosphate(CP),and acetylcodeine(AC) was established by CE-ECL with ionic liquid(1-butyl-3-methylimidazolium tetrafluoroborate,BMIMBF4). The conditions for the CE separation,ECL detection and the effect of ionic liquid(IL) were systematically investigated. Under the optimal conditions,the four analytes were well separated within 8 min using BMIMBF4 as additive in the electrophoretic buffer. The concentration detection limits of ME,thebaine,CP,and AC were 2.1×10-8,1.4×10-7,6.3×10-8,and 3.6×10-8 mol/L(S/N=3),respectively. The limits of quantitation(LOQ,S/N=10) in real human urine samples were 7.6×10-7 mol/L for ME,3.6 ×10-6 mol/L for thebaine,6.5×10-7 mol/L for CP,and 4.6×10-7 mol/L for AC,respectively. The recoveries of four alkaloids at different levels in human urine samples were between 90.0% and 103.5%. The developed method was successfully applied to the determination of four drug alkaloids in human urine samples.
