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嗜碱芽孢杆菌电转化方法的优化 被引量:1
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作者 成堃 路福平 +1 位作者 黎明 梁晓梅 《中国酿造》 CAS 北大核心 2010年第3期99-101,共3页
优化了嗜碱芽孢杆菌(Bacillus alcalophilus)TCCC11263的电转化条件,电转化条件包括细胞生长状态、电击场强、电击缓冲液组成、质粒DNA浓度。建立了一种适用于B.alcalophilus TCCC11263的电转化体系。结果为:B.alcalophilus TCCC11263... 优化了嗜碱芽孢杆菌(Bacillus alcalophilus)TCCC11263的电转化条件,电转化条件包括细胞生长状态、电击场强、电击缓冲液组成、质粒DNA浓度。建立了一种适用于B.alcalophilus TCCC11263的电转化体系。结果为:B.alcalophilus TCCC11263培养至OD600为1.0时,电转化效率最高,DNA的转化子数达0.14×103个转化子/μg;用含0.5mol山梨醇、0.5mol甘露醇、10%甘油的电击缓冲液洗涤细胞,在场强21kV/cm、电阻200Ω,电容25μF的电击条件下,加入0.7μg质粒DNA,电转化效率达到1.5×103个转化子/μgDNA。 展开更多
关键词 嗜碱芽孢杆菌 电转参数
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电脉冲介导基因转移效率的实验研究 被引量:7
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作者 张国华 沈冬 +3 位作者 金彩科 谭小凡 陈光慧 汤健 《中华医学杂志》 CAS CSCD 北大核心 2001年第15期937-940,共4页
目的 研究电脉冲介导的基因转移效率及其最佳的基因转移的电脉冲参数。方法 用微量注射器将pcD2 /LacZ质粒 10 μg ,在昆明小鼠的股四头肌注射 ,1~ 2min内在注射部位给予不同参数的电脉冲刺激 (不同的电脉冲参数每组 10只小鼠 ) ,然... 目的 研究电脉冲介导的基因转移效率及其最佳的基因转移的电脉冲参数。方法 用微量注射器将pcD2 /LacZ质粒 10 μg ,在昆明小鼠的股四头肌注射 ,1~ 2min内在注射部位给予不同参数的电脉冲刺激 (不同的电脉冲参数每组 10只小鼠 ) ,然后进行 β 半乳糖苷酶活性的测定或酶组织化学染色。同时设空白对照组及单纯注射组。结果 电脉冲可明显增加LacZ基因的表达 ,电脉冲组的β 半乳糖苷酶活性 (131.6U/mg± 86 .5U/mg蛋白 )是单纯注射组 (4 .9U/mg± 1.0U/mg蛋白 )的 30倍(P <0 .0 5 )。组织化学染色结果表明电脉冲组肌肉组织中 β 半乳糖苷酶蓝色颗粒的数目和染色的程度均明显高于单纯肌肉注射组。当电脉冲参数电压 2 0 0V/cm ,波宽 40ms,脉冲次数 6次和频率 1Hz时 ,可获得最高的基因表达效率。结论 在最佳的电脉冲参数条件下 ,电脉冲介导的基因转移可获得较高的基因表达。 展开更多
关键词 基因 基因疗法 脉冲介导 基因移方法 电转参数
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Multi-converter Approach to Higher Power Density DC-DC Converter for 380 V DC Distribution System 被引量:1
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作者 YusukeHayashi 《Journal of Energy and Power Engineering》 2013年第7期1331-1343,共13页
Multi-converter approach based on the series and parallel connection topology of modular power converters has been proposed to realize higher power density DC-DC converter. The availability of the proposed approach ha... Multi-converter approach based on the series and parallel connection topology of modular power converters has been proposed to realize higher power density DC-DC converter. The availability of the proposed approach has been verified through the design consideration and the experiment. The design consideration for two DC-DC converters has been carried out by utilizing the power converter exact loss simulator, and the design parameters to maximize their power densities have been extracted taking the trade-off between the conversion efficiency and the power density into account. The prototypes of a 2,400 W, 256-384 V boost chopper using SiC-MOSFETs and a 300 W, 32-48 V GaN-FETs boost chopper have been also developed based on the design. The SiC chopper achieved the efficiency of 97.8% and the power density of 12,8 W/cm3, and the GaN chopper accomplished 98.9% and 18.6 W/cm3 in the experiment. These results show the validity of the design and the availability of the proposed approach. The multi-converter approach enables the cost reduction of the modular power converters, and contributes to realizing the widespread use of power electronics converters in the future 380 V DC distribution system. 展开更多
关键词 DC distribution system DC power supply DC-DC converter power density silicon carbide gallium nitride.
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Steering mechanical analysis for lunar rover wheel
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作者 刘吉成 《High Technology Letters》 EI CAS 2009年第4期423-428,共6页
Facing the requirement of establishing a steering mechanical model for the wheel configuration design,selection of steering motors, dynamic analysis and simulation of the lunar rover, shear force beneaththe steering w... Facing the requirement of establishing a steering mechanical model for the wheel configuration design,selection of steering motors, dynamic analysis and simulation of the lunar rover, shear force beneaththe steering wheel, bulldozing resistance acting on steering wheel rims and side surfaces respectively areconducted on the basis of the wheel-loose soil interaction. The quantitative relation between steering resistancemoment (SRM) and steering radius, dimension of the wheel, soil parameters is established. Tovalidate the model, a single-wheel test bed is employed to test the steering performance of a wheel with0.15735m radius and 0.165m width when the steering radius is 0.00m, 0.04m, 0.08m, 0.12m and0.16m, respectively. The SRM is approached asymptotically with the increasing steering angle and almostproportional to the steering radius. The theoretical results of SRM are compact with the experimental results,which shows that the steering model can predict the experimental results well. 展开更多
关键词 terramechanics steering mechanical model steering radius lunar rover WHEEL
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Influence of Design Parameters on Cogging Torque in Directly Driven Permanent Magnet Synchronous Wind Generators
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作者 Q.L. Deng S.D. Huang F. Xiao 《Journal of Energy and Power Engineering》 2010年第7期42-47,共6页
In order to reduce the cogging torque, this paper investigates the influence of some parameters on the cogging torque developed by directly driven permanent magnet synchronous wind generators. Based on the remanent ma... In order to reduce the cogging torque, this paper investigates the influence of some parameters on the cogging torque developed by directly driven permanent magnet synchronous wind generators. Based on the remanent magnetic flux densities, the cogging torque is computed by using finite element method. It is shown that many parameters have influence on cogging torque and the slot and pole number combination has a significant effect on cogging torque. A simple factor has been introduced to indicate the effect of the slot and pole number combination. Some practical experience to reduce the cogging torque was applied to 2 MW three phase permanent magnet synchronous generator at rated speed of 37.5 rpm for wind energy conversion. The simulation and experiment results verify the effect of the proposed method. 展开更多
关键词 Cogging torque permanent magnet synchronous generator electric machine design.
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