淫羊藿苷伍用三七总皂苷对Aβ-淀粉肽_(25-35)侧脑室注射所致大鼠行为异常及脑组织AchE活性的影响

目的研究淫羊藿苷(icariin,ICA)伍用三七总皂苷(Panaxnotoginsengsaponins,PNS)对Aβ淀粉肽2535(betaamyloidpeptide2535,βAP2535)大鼠侧脑室注射空间学习和记忆障碍的影响。方法取经跳台法和八臂迷宫法筛选的正常大鼠采用侧脑室注射βAP2535,制成阿尔茨海默病(Alzheimer'sdisease,AD)大鼠模型,应用跳台法和八臂电迷宫法判断给药前后大鼠的空间学习和记忆能力;采用化学比色法测定脑组织中乙酰胆碱酯酶(acetylcholinesterase,AchE)活性。结果与溶剂组大鼠相比,模型大鼠八臂电迷宫错误次数明显增加(P<0.05),跳台学习和记忆错误次数明显增加(P<0.01)。大鼠造模前后连续igICA+PNS5+40,10+80mg/kg21d后,模型大鼠上述行为学指标得到明显改善(P<0.05),脑组织AchE活性降低(P<0.05)。结论ICA伍用PNS(5+40,10+80mg/kg)对βAP2535侧脑室注射所致大鼠空间学习和记忆障碍有显著的预防和治疗作用,该作用与降低脑组织中AchE活性呈相关性。

醒脑静注射液促智作用及其机制初探

目的 :研究醒脑静注射液 (XNJI)的促记忆作用及与脑内胆碱系统的关系。方法 :正常小鼠空间辨别学习能力的测试采用Y迷宫法 ;比色法检测脑组织乙酰胆碱酯酶的活性。结果 :XNJI对正常小鼠的空间辨别学习能力无明显影响 ,但在一定的剂量范围内可抑制脑内乙酰胆碱酯酶的活性。结论 :XNJI中存在抗肿碱酯酶的成分 ,但其作用可能受到其他组分的干扰。

Effect of Electroacupuncture on Learning and Memory in Rats with Vascular Dementia

Objective：To observe the effect of electroacupuncture on learning and memory in rats with vascular dementia.Methods：Seventy-two SD rats were randomly divided into 4 groups,a normal group,a sham operation group,a model group and an electroacupuncture （EA） pretreatment group.Modified intraluminal embolism method with nylon thread was applied to make rat model of vascular dementia,and the ability of learning and memory was observed in Morris water maze.Results：The average time of escape and latency in the rats of model group in the water maze test was prolonged,with significant difference compared with that in the sham group （P〈0.01）.The average time of escape and latency and navigation test results of rats pretreated by EA had statistically significant difference compared with those of the model group （P〈0.01）.Conclusion：EA pretreatment can significantly improve the ability of learning and memory of the rats with vascular dementia.