Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock olig...Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopusjaponicus, a sea cucumber, and the lysozyme and superoxide dismutase （SOD） activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal＇s coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides （P〈0.05）. The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysacchafide, and burdock oligosaccharide on day 1 and day 2 after the injection （P〈0.05）. The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection （P〈0.05）, while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.展开更多
基金Supported by the National Key Technology Research and Development Program (863 Program, No. 2006BAD09A06)the Special Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes (No. 02-2007B03)
文摘Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides were injected into Apostichopusjaponicus, a sea cucumber, and the lysozyme and superoxide dismutase （SOD） activities of the coelomic fluid and epidermal slime were monitored in six consecutive days. The results show that the lysozyme activity of the animal＇s coelomic fluid was significantly stimulated on day 2, day 4 and day 6 after the injection of the saccharides （P〈0.05）. The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime was significantly increased by chitosana, yeast polysaccharide, seaweed polysacchafide, and burdock oligosaccharide on day 1 and day 2 after the injection （P〈0.05）. The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection （P〈0.05）, while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.
