[Objective] The aim was to explore the effect of tomato＇s resistance to Botrytis cinerea induced by exogenous Chitosan.[Method] The leaf spraying method was used to determine the induced resistance effect of Chitosan...[Objective] The aim was to explore the effect of tomato＇s resistance to Botrytis cinerea induced by exogenous Chitosan.[Method] The leaf spraying method was used to determine the induced resistance effect of Chitosan to tomato B.cinerea and the chlorophyll content,soluble protein,soluble sugar,proline influence,the activities of peroxidase（POD）,malondialdehyde（MDA）and the Proline content in leaves of tomato.[Result] The induced resistance of Chitosan reached 58.26% at 14th d after treatment;Compared with the B.cinerea stress group,the chlorophyll content in leaves of tomato seedlings of B.cinerea stress ＋ Chitosan group had increased by 34.63%,while that of soluble protein content,soluble sugar content,Pro content and POD activity was 5.30%,10.83%,16.21% and 16.88%,respectively（except for the MDA content,which was decreased by 16.54%）.[Conclusion] Exogenous Chitosan could improve photosynthetic efficiency and protect enzymes activity to improve the resistance of tomato seedlings to B.cinerea.展开更多
[Objective] This study aimed to inveseigate the effects of chitosan on physiological characteristics of tomato seedlings under salt stress. [Method] Under salt stress of 200 mmol/L NaCI, foliar spraying induction meth...[Objective] This study aimed to inveseigate the effects of chitosan on physiological characteristics of tomato seedlings under salt stress. [Method] Under salt stress of 200 mmol/L NaCI, foliar spraying induction method was used to explore the physiological role of chitosan in salt resistance of tomato seedlings with a concentration of 150 mg/L [Result] Chlorophyll content, free proline content, SOD activity and CAT activity of tomato seedlings treated with CTS ＋ salt stress increased by 26.8%, 10.7%, 10% and 58.3%, respectively, compared with that in salt stress treatment group, while MDA content had decreased by 62.5%. [Conclusion] Chitosan could delay the decline of chlorophyll content, reduce membrane lipid peroxidation, improve osmotic adjustment ability of cells, enhance the activity of protective enzyme and improve the physiological synergisms on salt resistance of tomato seedlings.展开更多
[Objective] The aim was to explore the effects of low night temperature treatment on sucrose synthesis ability of tomato leaves in seedling stage.[Method] Effects of low night temperature of 6 ℃（with 15 ℃ as contro...[Objective] The aim was to explore the effects of low night temperature treatment on sucrose synthesis ability of tomato leaves in seedling stage.[Method] Effects of low night temperature of 6 ℃（with 15 ℃ as control）on the sucrose synthesis ability of tomato leaves were studied after 1,3,5,7 d of treatment.[Result] The contents of fructose,glucose and sucrose in tomato leaves were higher than control at 6：00 during the treatment of low night temperature,but in contrast at 11：00.The contents of fructose,glucose and sucrose in tomato leaves of low night temperature treatment and control group at 6：00 were lower than that at 11：00.The content of starch in tomato leaves was higher than control at 6：00 and 11：00 during the treatment.And the activities of sugar metabolism enzymes of tomato were changed by the treatment of low night temperature.The activities of acid invertase（AI）and neutral invertase（NI）were higher than those of control,while the activities of sucrose synthase（SS）and sucrose phosphate synthase（SPS）were lower than control at 6：00 and 11：00 during the treatment.The activity of all the enzymes at 11：00 of low night temperature treatment plants treated for 1,3,5,7 d was higher than that at 6：00.Compared to 6：00,the invertase activity of the control plant decreased,while the synthetase activity increased.The activity of sucrose synthase（SS）was decreased for treated plants,and the increase amount of sucrose content in leaves was lower than that of control at 11：00 during the treatment,indicating that the sucrose synthesis ability of tomato leaves was decreased by the treatment of low night temperature.[Conclusion] The results in this study had provided theoretical basis for the environmental control of high-yield cultivation of tomato.展开更多
To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to c...To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to conduct field efficacy tests for Lentinan ＋ Gibberellin-heteroauxin-brassinolide （GHB） and Ningnanmycin ＋ GHB. The results showed that when the virus diseases in greenhouse tomato were serious, 0.5% Lentinan AS 3 000 ml ＋ GHB WP 600 g and 8% Ningnanmycin AS 900 ml ＋ GHB WP 600 g per hectare were applied 4 times with an interval of 7 days, and the control effects on the 10th, 17th, 26th and 35th day were above 88%, 87%, 78% and 67%, respectively. The difference in control effect of the two biological pesticides was insignificant, while their control effects were all significantly better than that of moroxydine hydrochloride （CK） ＋ GHB. The obtained results indicated that Lentinan ＋ GHB and Ningnanmycin ＋ GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production.展开更多
In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO ...In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO (0.05%, 0.10%, 0.20%, 0.30% and 0.50%) after drought stress was simulated by exposing the roots to 0.6 molL-1 PEG-6000 solution for 6 h. Changes in biomass, electrolyte leakage and malondialdehyde (MDA), free proline, total soluble sugars (TSS) and abscisic acid (ABA), the enzyme activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.展开更多
文摘[Objective] The aim was to explore the effect of tomato＇s resistance to Botrytis cinerea induced by exogenous Chitosan.[Method] The leaf spraying method was used to determine the induced resistance effect of Chitosan to tomato B.cinerea and the chlorophyll content,soluble protein,soluble sugar,proline influence,the activities of peroxidase（POD）,malondialdehyde（MDA）and the Proline content in leaves of tomato.[Result] The induced resistance of Chitosan reached 58.26% at 14th d after treatment;Compared with the B.cinerea stress group,the chlorophyll content in leaves of tomato seedlings of B.cinerea stress ＋ Chitosan group had increased by 34.63%,while that of soluble protein content,soluble sugar content,Pro content and POD activity was 5.30%,10.83%,16.21% and 16.88%,respectively（except for the MDA content,which was decreased by 16.54%）.[Conclusion] Exogenous Chitosan could improve photosynthetic efficiency and protect enzymes activity to improve the resistance of tomato seedlings to B.cinerea.
文摘[Objective] This study aimed to inveseigate the effects of chitosan on physiological characteristics of tomato seedlings under salt stress. [Method] Under salt stress of 200 mmol/L NaCI, foliar spraying induction method was used to explore the physiological role of chitosan in salt resistance of tomato seedlings with a concentration of 150 mg/L [Result] Chlorophyll content, free proline content, SOD activity and CAT activity of tomato seedlings treated with CTS ＋ salt stress increased by 26.8%, 10.7%, 10% and 58.3%, respectively, compared with that in salt stress treatment group, while MDA content had decreased by 62.5%. [Conclusion] Chitosan could delay the decline of chlorophyll content, reduce membrane lipid peroxidation, improve osmotic adjustment ability of cells, enhance the activity of protective enzyme and improve the physiological synergisms on salt resistance of tomato seedlings.
基金Supported by National Natural Science Foundation of China(30170640)~~
文摘[Objective] The aim was to explore the effects of low night temperature treatment on sucrose synthesis ability of tomato leaves in seedling stage.[Method] Effects of low night temperature of 6 ℃（with 15 ℃ as control）on the sucrose synthesis ability of tomato leaves were studied after 1,3,5,7 d of treatment.[Result] The contents of fructose,glucose and sucrose in tomato leaves were higher than control at 6：00 during the treatment of low night temperature,but in contrast at 11：00.The contents of fructose,glucose and sucrose in tomato leaves of low night temperature treatment and control group at 6：00 were lower than that at 11：00.The content of starch in tomato leaves was higher than control at 6：00 and 11：00 during the treatment.And the activities of sugar metabolism enzymes of tomato were changed by the treatment of low night temperature.The activities of acid invertase（AI）and neutral invertase（NI）were higher than those of control,while the activities of sucrose synthase（SS）and sucrose phosphate synthase（SPS）were lower than control at 6：00 and 11：00 during the treatment.The activity of all the enzymes at 11：00 of low night temperature treatment plants treated for 1,3,5,7 d was higher than that at 6：00.Compared to 6：00,the invertase activity of the control plant decreased,while the synthetase activity increased.The activity of sucrose synthase（SS）was decreased for treated plants,and the increase amount of sucrose content in leaves was lower than that of control at 11：00 during the treatment,indicating that the sucrose synthesis ability of tomato leaves was decreased by the treatment of low night temperature.[Conclusion] The results in this study had provided theoretical basis for the environmental control of high-yield cultivation of tomato.
文摘To alleviate the damage of tomato virus diseases, reduce chemical pesticide consumption and residue, and prevent environmental pollution, forecasting, randomized block design and statistical analysis were adopted to conduct field efficacy tests for Lentinan ＋ Gibberellin-heteroauxin-brassinolide （GHB） and Ningnanmycin ＋ GHB. The results showed that when the virus diseases in greenhouse tomato were serious, 0.5% Lentinan AS 3 000 ml ＋ GHB WP 600 g and 8% Ningnanmycin AS 900 ml ＋ GHB WP 600 g per hectare were applied 4 times with an interval of 7 days, and the control effects on the 10th, 17th, 26th and 35th day were above 88%, 87%, 78% and 67%, respectively. The difference in control effect of the two biological pesticides was insignificant, while their control effects were all significantly better than that of moroxydine hydrochloride （CK） ＋ GHB. The obtained results indicated that Lentinan ＋ GHB and Ningnanmycin ＋ GHB were the ideal biological pesticides, which could be used not only for controlling tomato virus diseases, but also for realizing modern, pollution-free, green and organic agricultural production.
基金supported by the National Natural Science Foundation of China (No. 30771646)Shandong Province Independent Innovation Project with the title of ‘Industrialization development of several special seaweeds biological products using integrated technologies’
文摘In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO (0.05%, 0.10%, 0.20%, 0.30% and 0.50%) after drought stress was simulated by exposing the roots to 0.6 molL-1 PEG-6000 solution for 6 h. Changes in biomass, electrolyte leakage and malondialdehyde (MDA), free proline, total soluble sugars (TSS) and abscisic acid (ABA), the enzyme activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.
