目的:观察舌下免疫治疗(SLIT)疗效与尘螨组分sIgE和sIgG4水平变化的相关性。方法:回顾性分析2018年4月~2021年10月就诊于某院过敏反应科并行SLIT的过敏性鼻炎患儿(n=37),根据治疗1年后症状和药物综合评分(CSMS)分为控制稳定组(n=25)和...目的:观察舌下免疫治疗(SLIT)疗效与尘螨组分sIgE和sIgG4水平变化的相关性。方法:回顾性分析2018年4月~2021年10月就诊于某院过敏反应科并行SLIT的过敏性鼻炎患儿(n=37),根据治疗1年后症状和药物综合评分(CSMS)分为控制稳定组(n=25)和控制不稳定组(n=12),比较两组患儿9种重要尘螨致敏蛋白组分(Derp1/Derf1、Derp2/Derf2、Derp5、Derp7、Derp10、Derp21和Derp23)sIgE和sIgG4的水平变化。结果:Derp1/Derf1和Derp2/Derf2为主要致敏蛋白组分,其治疗前sIgE阳性率为86.5%~94.6%,其中Der p 2的sIgE浓度高于Derp1(P<0.0001)、Derp5(P<0.001)、Derp7(P<0.001)、Derp10(P<0.001)、Derp21(P<0.01);Derf2的sIgE浓度高于Derf1(P<0.0001)、Derp5(P<0.0001)、Derp7(P<0.0001)、Der p 10(P<0.0001)、Der p 21(P<0.0001)和Der p 23(P<0.01)。治疗前Der f 2的sIgG4浓度高于其他组分(P<0.0001),控制不稳定组中Der f 1的sIgG4阳性率(33.3%)高于控制稳定组(4.0%,P<0.05),且控制不稳定组中Derf1和Derp10的sIgG4浓度高于控制稳定组(P<0.05)。治疗1年后,控制稳定组患儿Der f 1和Der p 2的sIgG4水平高于治疗前(P<0.05)。结论:尘螨致敏过敏性鼻炎患儿应用SLIT治疗后,Der f 1和Der p 2的sIgG4浓度升高明显,提示SLIT疗效较好,为临床评估SLIT疗效提供了线索。展开更多
Aiming at starting the ball rolling and contributing humble effort to promote CTM （Chinese traditional medicine）, we performed the present study to assess the therapy response of Chinese herbal decoction compared to...Aiming at starting the ball rolling and contributing humble effort to promote CTM （Chinese traditional medicine）, we performed the present study to assess the therapy response of Chinese herbal decoction compared to conventional therapy on critical ill patients of advanced liver cancer. A total of 6 patients （1 female and 5 males） with histologically confirmed liver cancer were included in this retrospective observational clinical trial. We administered Chinese medicine （Gan Decoction, mixed with a variety of effective herbal components） to help them to recover from poor condition. In the meantime, conventional treatment of surgical resection and artery catheterization chemotherapy was applied in cases compared. In 3 cases of CTM combined treatment, the tumor marker level decreased. Residual intrahepatic metastatic sites reduced according to ultrasonography/CT imaging, and the patients felt free from the complaint of abdominal discomfort. The quality of life has been improved, we managed to have prolonged the PFS （Progression-Free-Survival） and TTP （Time-to-Progression） from the onset to date. While in 3 cases with conventional treatment only of surgical resection and artery catheterization chemotherapy, we were not able to decrease the level of tumor marker, metastatic lesions increased according to ultrasonography/CT imaging, and the patient＇s condition worsen more. We failed in having prolonged the PFS and TTP in the compared cases of conventional treatment only. The retrospective clinical study showed no OS （overall survival） benefit for liver cancer patients treated with Gan Decoction, while the QOL （quality of life） evaluation seemed to predict survival better. Chinese herbs might be an additional choice with its better benefits and tolerability in the treatment of primary liver cancer.展开更多
Although patients with drug-resistant temporal lobe epilepsy(TLE)achieve good seizure control with resective surgery combined with anti-epileptic drugs(AEDs)treatment,it is still not clear when to withdraw AEDs after ...Although patients with drug-resistant temporal lobe epilepsy(TLE)achieve good seizure control with resective surgery combined with anti-epileptic drugs(AEDs)treatment,it is still not clear when to withdraw AEDs after the surgery due to a lack of objective strategy for assessing the surgery outcome.Our aim is to identify objective and efficient biomarkers for assessment of surgery outcome to guide postoperative pharmacotherapy.Here,we detected 469 proteins differentially expressed between pre-and post-operative sera of TLE patients with postoperative seizure freedom,and identified six candidate proteins(HBA1,HBB,PRDX2,CA1,CAT and BLVRB)for validation.These six proteins were significantly reduced within three months after surgery,indicating these proteins may be potential biomarkers to assess the surgery outcome for TLE patients in the early stage.Interestingly,we found HBA1 to HBB ratio was superior to individual proteins to evaluate the postoperative surgery outcome with 90.0%sensitivity,90%specificity and area under the curve of 0.960.We also suppose these proteins were derived from epilepsy foci due to the rat experiment results that HBA1,HBB,PRDX2,CA1 and CAT were highly expressed in cerebrospinal fluid of epilepsy rat models.Additionally,HBA1,HBB,PRDX2,CA1 and BLVRB proteins were highly expressed in the sera of TLE patients with postoperative seizure freedom when compared to TLE patients with postoperative seizure and patients with other types of epilepsy,suggesting surgery outcome or the original sites of seizure might be assessed before the operation through monitoring these protein levels in the sera.Our study provides experimental data for establishing objective indicators for assessing seizure freedom after resective surgery and also proposed potential markers for specific diagnoses of TLE and prediction of TLE surgery outcome.展开更多
文摘目的:观察舌下免疫治疗(SLIT)疗效与尘螨组分sIgE和sIgG4水平变化的相关性。方法:回顾性分析2018年4月~2021年10月就诊于某院过敏反应科并行SLIT的过敏性鼻炎患儿(n=37),根据治疗1年后症状和药物综合评分(CSMS)分为控制稳定组(n=25)和控制不稳定组(n=12),比较两组患儿9种重要尘螨致敏蛋白组分(Derp1/Derf1、Derp2/Derf2、Derp5、Derp7、Derp10、Derp21和Derp23)sIgE和sIgG4的水平变化。结果:Derp1/Derf1和Derp2/Derf2为主要致敏蛋白组分,其治疗前sIgE阳性率为86.5%~94.6%,其中Der p 2的sIgE浓度高于Derp1(P<0.0001)、Derp5(P<0.001)、Derp7(P<0.001)、Derp10(P<0.001)、Derp21(P<0.01);Derf2的sIgE浓度高于Derf1(P<0.0001)、Derp5(P<0.0001)、Derp7(P<0.0001)、Der p 10(P<0.0001)、Der p 21(P<0.0001)和Der p 23(P<0.01)。治疗前Der f 2的sIgG4浓度高于其他组分(P<0.0001),控制不稳定组中Der f 1的sIgG4阳性率(33.3%)高于控制稳定组(4.0%,P<0.05),且控制不稳定组中Derf1和Derp10的sIgG4浓度高于控制稳定组(P<0.05)。治疗1年后,控制稳定组患儿Der f 1和Der p 2的sIgG4水平高于治疗前(P<0.05)。结论:尘螨致敏过敏性鼻炎患儿应用SLIT治疗后,Der f 1和Der p 2的sIgG4浓度升高明显,提示SLIT疗效较好,为临床评估SLIT疗效提供了线索。
文摘Aiming at starting the ball rolling and contributing humble effort to promote CTM （Chinese traditional medicine）, we performed the present study to assess the therapy response of Chinese herbal decoction compared to conventional therapy on critical ill patients of advanced liver cancer. A total of 6 patients （1 female and 5 males） with histologically confirmed liver cancer were included in this retrospective observational clinical trial. We administered Chinese medicine （Gan Decoction, mixed with a variety of effective herbal components） to help them to recover from poor condition. In the meantime, conventional treatment of surgical resection and artery catheterization chemotherapy was applied in cases compared. In 3 cases of CTM combined treatment, the tumor marker level decreased. Residual intrahepatic metastatic sites reduced according to ultrasonography/CT imaging, and the patients felt free from the complaint of abdominal discomfort. The quality of life has been improved, we managed to have prolonged the PFS （Progression-Free-Survival） and TTP （Time-to-Progression） from the onset to date. While in 3 cases with conventional treatment only of surgical resection and artery catheterization chemotherapy, we were not able to decrease the level of tumor marker, metastatic lesions increased according to ultrasonography/CT imaging, and the patient＇s condition worsen more. We failed in having prolonged the PFS and TTP in the compared cases of conventional treatment only. The retrospective clinical study showed no OS （overall survival） benefit for liver cancer patients treated with Gan Decoction, while the QOL （quality of life） evaluation seemed to predict survival better. Chinese herbs might be an additional choice with its better benefits and tolerability in the treatment of primary liver cancer.
基金grant(973 Program,Grant No.015CB559200 to Z.H.)from the Ministry of Science and Technology of Chinagrant(Grant No.31871083 to Z.H.)from the National Natural Science Foundation of Chinagrant(Grant No.7182087 to Z.H.)from the Beijing Natural Science Foundation.
文摘Although patients with drug-resistant temporal lobe epilepsy(TLE)achieve good seizure control with resective surgery combined with anti-epileptic drugs(AEDs)treatment,it is still not clear when to withdraw AEDs after the surgery due to a lack of objective strategy for assessing the surgery outcome.Our aim is to identify objective and efficient biomarkers for assessment of surgery outcome to guide postoperative pharmacotherapy.Here,we detected 469 proteins differentially expressed between pre-and post-operative sera of TLE patients with postoperative seizure freedom,and identified six candidate proteins(HBA1,HBB,PRDX2,CA1,CAT and BLVRB)for validation.These six proteins were significantly reduced within three months after surgery,indicating these proteins may be potential biomarkers to assess the surgery outcome for TLE patients in the early stage.Interestingly,we found HBA1 to HBB ratio was superior to individual proteins to evaluate the postoperative surgery outcome with 90.0%sensitivity,90%specificity and area under the curve of 0.960.We also suppose these proteins were derived from epilepsy foci due to the rat experiment results that HBA1,HBB,PRDX2,CA1 and CAT were highly expressed in cerebrospinal fluid of epilepsy rat models.Additionally,HBA1,HBB,PRDX2,CA1 and BLVRB proteins were highly expressed in the sera of TLE patients with postoperative seizure freedom when compared to TLE patients with postoperative seizure and patients with other types of epilepsy,suggesting surgery outcome or the original sites of seizure might be assessed before the operation through monitoring these protein levels in the sera.Our study provides experimental data for establishing objective indicators for assessing seizure freedom after resective surgery and also proposed potential markers for specific diagnoses of TLE and prediction of TLE surgery outcome.
