目的评价美国Inverness medical professional diagnostics公司生产的疟原虫检测试剂卡的临床应用效果。方法以镜检方法作为金标准比较BinaxNOW疟原虫检测试剂卡临床应用效果。结果 400份样本用镜检方法和BinaxNOW疟原虫检测试剂卡...目的评价美国Inverness medical professional diagnostics公司生产的疟原虫检测试剂卡的临床应用效果。方法以镜检方法作为金标准比较BinaxNOW疟原虫检测试剂卡临床应用效果。结果 400份样本用镜检方法和BinaxNOW疟原虫检测试剂卡诊断,镜检法检出阳性138份,其中恶性疟77份、间日疟61份;疟原虫检测试剂卡检出阳性139例,其中恶性疟71份,间日疟68份。试剂卡检测疟疾的敏感度为98.55%,特异度为98.85%,检测恶性疟的灵敏度为87.01%、特异度为98.76%,检测间日疟的敏感度为93.44%、特异度为96.76%。存在交叉反应。结论BinaxNOW疟原虫检测试剂卡诊断疟疾敏感性与特异性高,操作简单,结果显示直观、快速,适合边远疟区无镜检能力的个体、乡村医生开展疟疾筛查。展开更多
疟疾是由疟原虫引起的蚊媒传染性疾病,具有分布广泛、传播迅速、潜伏期长等特点,其直接关系到人类的健康、经济的发展以及社会的稳定.疟疾的快速准确检测是降低疟疾的病死率和控制疟疾传播的关键.目前已有研究利用深度学习算法实现对疟...疟疾是由疟原虫引起的蚊媒传染性疾病,具有分布广泛、传播迅速、潜伏期长等特点,其直接关系到人类的健康、经济的发展以及社会的稳定.疟疾的快速准确检测是降低疟疾的病死率和控制疟疾传播的关键.目前已有研究利用深度学习算法实现对疟原虫的检测,但开发疟疾临床诊断的人工智能系统仍然存在挑战.本研究基于深度学习中的多尺度注意力机制,构建了基于人工智能的疟疾诊断目标检测模型(artificial intelligence-based object detection model for malaria diagnosis,AIM).同时,本研究利用智能手机与光学显微镜收集薄血涂片图像,创建了疟原虫薄血涂片图像数据集(Smart Malaria NET),并用于AIM模型的训练与评估.结果表明,AIM模型的Accuracy为94.49%,Precision为94.54%,Recall为94.49%,F1-score为94.50%,AUC(area under curve)为98.11%,各项评价指标均优于现有的VGG和Res Net模型.该人工智能的疟疾诊断目标检测模型有助于提高缺乏镜检人员地区的疟疾诊断能力,为全球疟疾防控提供“中国技术”与“中国方案”.展开更多
The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chai...The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chain reaction （PCR） was performed for further confirmation. A total of 50 blood samples were collected from poultry birds showing the malarial symptoms. The results of blood smear methods showed two samples （4%） were infected with genus Plasmodium, whereas the PCR analysis showed four （8%） positive samples. These results confirm that the PCR is more sensitive method for detecting the Plasmodium when compared with conventional methods, and the microscopy diagnosed 50% false negative results that were confirmed by PCR.展开更多
Objective To detect malaria DNA in mosquitoes.Methods A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used.Results In labora...Objective To detect malaria DNA in mosquitoes.Methods A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used.Results In laboratory-infected mosquitoes, nested PCR could detect as few as 3 sporozoites or 1 infected mosquito mixed in a group of 99 normal ones. Furthermore, no specific 121?bp band was seen with DNA templates from other malaria parasites or negative mosquitoes.Conclusion Sensitivity and specificity obtained indicated an advantage of the nested PCR over DNA probes or direct PCR for the detection of Plasmodium vivax sporozoites in mosquitoes with low-grade parasitic infections.展开更多
文摘目的评价美国Inverness medical professional diagnostics公司生产的疟原虫检测试剂卡的临床应用效果。方法以镜检方法作为金标准比较BinaxNOW疟原虫检测试剂卡临床应用效果。结果 400份样本用镜检方法和BinaxNOW疟原虫检测试剂卡诊断,镜检法检出阳性138份,其中恶性疟77份、间日疟61份;疟原虫检测试剂卡检出阳性139例,其中恶性疟71份,间日疟68份。试剂卡检测疟疾的敏感度为98.55%,特异度为98.85%,检测恶性疟的灵敏度为87.01%、特异度为98.76%,检测间日疟的敏感度为93.44%、特异度为96.76%。存在交叉反应。结论BinaxNOW疟原虫检测试剂卡诊断疟疾敏感性与特异性高,操作简单,结果显示直观、快速,适合边远疟区无镜检能力的个体、乡村医生开展疟疾筛查。
文摘疟疾是由疟原虫引起的蚊媒传染性疾病,具有分布广泛、传播迅速、潜伏期长等特点,其直接关系到人类的健康、经济的发展以及社会的稳定.疟疾的快速准确检测是降低疟疾的病死率和控制疟疾传播的关键.目前已有研究利用深度学习算法实现对疟原虫的检测,但开发疟疾临床诊断的人工智能系统仍然存在挑战.本研究基于深度学习中的多尺度注意力机制,构建了基于人工智能的疟疾诊断目标检测模型(artificial intelligence-based object detection model for malaria diagnosis,AIM).同时,本研究利用智能手机与光学显微镜收集薄血涂片图像,创建了疟原虫薄血涂片图像数据集(Smart Malaria NET),并用于AIM模型的训练与评估.结果表明,AIM模型的Accuracy为94.49%,Precision为94.54%,Recall为94.49%,F1-score为94.50%,AUC(area under curve)为98.11%,各项评价指标均优于现有的VGG和Res Net模型.该人工智能的疟疾诊断目标检测模型有助于提高缺乏镜检人员地区的疟疾诊断能力,为全球疟疾防控提供“中国技术”与“中国方案”.
文摘The present study was conducted to detect the Plasmodium, the causative agent of malaria in domestic poultry birds. Blood smear method was used as the conventional method for the detection, whereas the polymerase chain reaction （PCR） was performed for further confirmation. A total of 50 blood samples were collected from poultry birds showing the malarial symptoms. The results of blood smear methods showed two samples （4%） were infected with genus Plasmodium, whereas the PCR analysis showed four （8%） positive samples. These results confirm that the PCR is more sensitive method for detecting the Plasmodium when compared with conventional methods, and the microscopy diagnosed 50% false negative results that were confirmed by PCR.
文摘Objective To detect malaria DNA in mosquitoes.Methods A nested polymerase chain reaction (nested PCR) procedure which amplifies a 121 bp DNA of a SSUrRNA gene specific to Plasmodium vivax was used.Results In laboratory-infected mosquitoes, nested PCR could detect as few as 3 sporozoites or 1 infected mosquito mixed in a group of 99 normal ones. Furthermore, no specific 121?bp band was seen with DNA templates from other malaria parasites or negative mosquitoes.Conclusion Sensitivity and specificity obtained indicated an advantage of the nested PCR over DNA probes or direct PCR for the detection of Plasmodium vivax sporozoites in mosquitoes with low-grade parasitic infections.
